CAJ | 학술논문

目的研究蝶酸(pterin acid, PTA)对天然蓖麻毒素(ricin)及重组蓖麻毒素A链蛋白(rRTA)的拮抗作用.方法用无细胞体系中荧光素酶合成抑制实验和体外细胞毒性实验评价PTA对全分子ricin和rRTA的拮抗效果.结果 PTA在两种体系中均显示出了对ricin和rRTA的拮抗作用,且呈剂量依赖性.结论利用PTA作为小分子探针,建立了针对ricin/RTA小分子抑制剂的体外生物学评价系统,为以后用于拮抗ricin/RTA的全新小分子化合物筛选奠定了基础.
목적 연구접산(pterin acid, PTA)대천연비마독소(ricin)급중조비마독소A련단백(rRTA)적길항작용.방법 용무세포체계중형광소매합성억제실험화체외세포독성실험평개PTA대전분자ricin화rRTA적길항효과.결과 PTA재량충체계중균현시출료대ricin화rRTA적길항작용,차정제량의뢰성.결론 이용PTA작위소분자탐침,건립료침대ricin/RTA소분자억제제적체외생물학평개계통,위이후용우길항ricin/RTA적전신소분자화합물사선전정료기출.
Objective To study the inhibitory effect of pterin acid (PTA) against ricin and recombinant ricin A chain protein. Methods Luciferase protein synthesis inhibition assay in a cell-free system and in vitro cytotoxicity experiments were performed to assess the biological activity of ricin and rRTA treated with PTA.Results The result showed that PTA could significantly inhibit the activity of ricin and rRTA in a dose-dependent manner.Conclusion PTA might be used as a small molecular probe to develop an evaluating system for ricin/RTA small molecular inhibitor in vitro. The cell-free system adopted in the current study could also serve as a necessary basis for screening some novel small molecular compounds against ricin and RTA in the future.