CAJ | 학술논문

目的探讨c-fos与c-jun两种原癌基因产物在溃疡与增生性瘢痕创面表达的特征与规律以及与不同组织修复结局发生的关系。方法 16例标本均取自于外科手术患者，其中增生性瘢痕8例，溃疡创面8例，另有正常对照皮肤5例取自增生性瘢痕患者作为对照。用免疫组化法（ABC法）检测c-fos与c-jun两种癌蛋白以及bFGF在三种组织切片的分布特征。结果在正常皮肤c-fos与c-jun的阳性表达主要见于表皮基底细胞和少量皮下成纤维细胞，但在相应部位c-jun的表达较c-fos为弱。在增生性瘢痕，c-fos与c-jun均出现强阳性表达，主要见于成纤维细胞。在溃疡组织，c-fos与c-jun的联合表达多见于毛细血管内皮细胞、部分炎症细胞以及成纤维细胞胞浆。结论增生性瘢痕与溃疡创面c-fos与c-jun表达量与部位同正常皮肤存在显著差异，提示这两种原癌基因产物在影响和调控组织修复中有重要作用。
목적탐토c-fos여c-jun량충원암기인산물재궤양여증생성반흔창면표체적특정여규률이급여불동조직수복결국발생적관계。 방법 16례표본균취자우외과수술환자，기중증생성반흔8례，궤양창면8례，령유정상대조피부5례취자증생성반흔환자작위대조。용면역조화법（ABC법）검측c-fos여c-jun량충암단백이급bFGF재삼충조직절편적분포특정。 결과재정상피부c-fos여c-jun적양성표체주요견우표피기저세포화소량피하성섬유세포，단재상응부위c-jun적표체교c-fos위약。재증생성반흔，c-fos여c-jun균출현강양성표체，주요견우성섬유세포。재궤양조직，c-fos여c-jun적연합표체다견우모세혈관내피세포、부분염증세포이급성섬유세포포장。 결론증생성반흔여궤양창면c-fos여c-jun표체량여부위동정상피부존재현저차이，제시저량충원암기인산물재영향화조공조직수복중유중요작용。
Objective　To explore the characteristics of oncoprotein expression of c-fos and c-jun in hypertrophic scars and chronic dermal ulcers and their regulation of basic fibroblast growth factor (bFGF). Methods　Tissues of hypertrophic scars (n=8), chronic dermal ulcers (n=8) and normal skin (n=5) were taken from 21 patients with burns and chronic dermal ulcers in operation. The ABC immunohistochemical method was used to characterize the gene product expression of c-fos, c-jun and bFGF in the above tissues. Results　In normal skin, both c-fos and c-jun protein expression and bFGF protein expression were observed. The signals of both oncoproteins were localized mainly in subcutaneous fibroblasts, but, positive expression of the bFGF protein was mainly in keratinocytes. In hypertrophic scars, positive expression of both oncoproteins could be found mainly in fibroblasts, but bFGF was mainly in fibroblasts and endothelial cells. In chronic dermal ulcers, endothelial cells, some of inflammatory cells and fibroblasts were positive for both of oncoproteins, but the expression of bFGF was only seen in fibroblasts and endothelial cells. Conclusions　The results indicate that the interaction between both oncoproteins and bFGF exists, and the regulating action between protooncogenes and bFGF is a major course in wound healing. The different expressions of c-fos and c-jun gene products play an important role in regulate bFGF action, thus affecting wound healing.