国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
7期
594-596
,共3页
赵见文%田军彪%李佃贵%张颜伟%杨丽静
趙見文%田軍彪%李佃貴%張顏偉%楊麗靜
조견문%전군표%리전귀%장안위%양려정
化浊解毒活血通络方%脑缺血再灌注损伤%炎症反应%IL-6%TNF-α
化濁解毒活血通絡方%腦缺血再灌註損傷%炎癥反應%IL-6%TNF-α
화탁해독활혈통락방%뇌결혈재관주손상%염증반응%IL-6%TNF-α
Huazhuo Jiedu Huoxue Tongluo recipe%Cerebral ischemia Reperfusion injury%Inflammatory reaction%IL-6%TNF-α
目的 观察化浊解毒活血通络方对小鼠脑缺血再灌注损伤的局部炎症反应的影响.方法 将50只昆明小鼠按随机数字表法分为脑缺血再灌注损伤模型组、中药高剂量组、中药低剂量组、尼莫地平组、假手术组,每组各10只,前四组采用线栓法制备小鼠右侧大脑中动脉脑缺血再灌注损伤模型.手术造模成功后脑缺血再灌注损伤模型组、假手术组灌服生理盐水,尼莫地平组灌服尼莫地平液(19.6 g/kg·d-1),中药高剂量组、中药低剂量组分别给予高(19.6 g/kg·d-1)、低剂量(9.8 g/kg·d-1)的化浊解毒活血通络方中药灌服.药物干预后用放免法检测各组小鼠血清IL-6、TNF-α的表达水平.结果 与模型组[(219.29±39.28)pg/ml、(1.325±0.236)ng/ml]比较,中药高剂量组、中药低剂量组、尼莫地平组小鼠血清中IL-6[分别为(120.69±49.23)pg/ml、(173.97±49.03)pg/ml、(170.88±47.06)pg/m1]和TNF-α[分别为(1.086±0.178)ng/ml、(0.937±0.105)ng/ml、(1.092±0.184)ng/ml]的表达水平均明显降低(P均<0.05).中药高剂量组与假手术组比较,差异无统计学意义(P>0.05).结论 化浊解毒活血通络方可抑制小鼠脑缺血再灌注损伤时炎症细胞因子的表达,减少白细胞的浸润.
目的 觀察化濁解毒活血通絡方對小鼠腦缺血再灌註損傷的跼部炎癥反應的影響.方法 將50隻昆明小鼠按隨機數字錶法分為腦缺血再灌註損傷模型組、中藥高劑量組、中藥低劑量組、尼莫地平組、假手術組,每組各10隻,前四組採用線栓法製備小鼠右側大腦中動脈腦缺血再灌註損傷模型.手術造模成功後腦缺血再灌註損傷模型組、假手術組灌服生理鹽水,尼莫地平組灌服尼莫地平液(19.6 g/kg·d-1),中藥高劑量組、中藥低劑量組分彆給予高(19.6 g/kg·d-1)、低劑量(9.8 g/kg·d-1)的化濁解毒活血通絡方中藥灌服.藥物榦預後用放免法檢測各組小鼠血清IL-6、TNF-α的錶達水平.結果 與模型組[(219.29±39.28)pg/ml、(1.325±0.236)ng/ml]比較,中藥高劑量組、中藥低劑量組、尼莫地平組小鼠血清中IL-6[分彆為(120.69±49.23)pg/ml、(173.97±49.03)pg/ml、(170.88±47.06)pg/m1]和TNF-α[分彆為(1.086±0.178)ng/ml、(0.937±0.105)ng/ml、(1.092±0.184)ng/ml]的錶達水平均明顯降低(P均<0.05).中藥高劑量組與假手術組比較,差異無統計學意義(P>0.05).結論 化濁解毒活血通絡方可抑製小鼠腦缺血再灌註損傷時炎癥細胞因子的錶達,減少白細胞的浸潤.
목적 관찰화탁해독활혈통락방대소서뇌결혈재관주손상적국부염증반응적영향.방법 장50지곤명소서안수궤수자표법분위뇌결혈재관주손상모형조、중약고제량조、중약저제량조、니막지평조、가수술조,매조각10지,전사조채용선전법제비소서우측대뇌중동맥뇌결혈재관주손상모형.수술조모성공후뇌결혈재관주손상모형조、가수술조관복생리염수,니막지평조관복니막지평액(19.6 g/kg·d-1),중약고제량조、중약저제량조분별급여고(19.6 g/kg·d-1)、저제량(9.8 g/kg·d-1)적화탁해독활혈통락방중약관복.약물간예후용방면법검측각조소서혈청IL-6、TNF-α적표체수평.결과 여모형조[(219.29±39.28)pg/ml、(1.325±0.236)ng/ml]비교,중약고제량조、중약저제량조、니막지평조소서혈청중IL-6[분별위(120.69±49.23)pg/ml、(173.97±49.03)pg/ml、(170.88±47.06)pg/m1]화TNF-α[분별위(1.086±0.178)ng/ml、(0.937±0.105)ng/ml、(1.092±0.184)ng/ml]적표체수평균명현강저(P균<0.05).중약고제량조여가수술조비교,차이무통계학의의(P>0.05).결론 화탁해독활혈통락방가억제소서뇌결혈재관주손상시염증세포인자적표체,감소백세포적침윤.
Objective To observe the effect of Huazhuo-Jiedu-Huoxue Tongluo recipe on local inflammation of cerebral ischemia reperfusion injury in mice. Method Fifty Kunming Mice were randomly divided into five groups: model group, high-dose group of TCM, low-dose group of TCM, nimodiping group and sham operation group. Each group had 10 mice. Mice models of the right middle cerebral artery CIRI in the first four groups were established through thread embolism method. Physiological saline was given to the mice in the model group and sham operation group. Nimodipine solution was given to the mice in the Nimodipine group. High and low dose of Huazhuo-Jiedu-Huoxue-Tongluo Chinese medicine decoction were given to the mice in the high dose of TCM group and low dose of TCM group respectively after the models were successfully established. To assess the expression level of IL-6 and TNF-α by radioimmunoassay after the treatment. Results Compared with model group [(219.29±39.28) pg/ml, (1.325 ± 0.236) ng/ml] , the level of IL-6 and TNF-α of high dose of TCM group[(120.69±49.23)pg/ml, (1.086±0.178)ng/ml], low dose of TCM group[(173.97±49.03)pg/ml, (0.937 ± 0.105)ng/ml]and nimodipine group[(170.88± 47.06)pg/ml, (1.092±0.184) ng/ml]decrease (P<0.05). There is no significant difference between the high dose of TCM group and the sham operation group (P>0.05). Conclusion Huazhuo-Jiedu-Huoxue-Tongluo recipe can restrain the expression of inflammatory cytokines and reduce the infiltrating of leukocytes in mice with cerebral ischemia reperfusion injury.
