中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2012年
1期
24-28
,共5页
杨艳%龚云辉%倪姗姗%周斌%宋雅平%张林%周容
楊豔%龔雲輝%倪姍姍%週斌%宋雅平%張林%週容
양염%공운휘%예산산%주빈%송아평%장림%주용
先兆子痫%骨保护素%多态现象,遗传
先兆子癇%骨保護素%多態現象,遺傳
선조자간%골보호소%다태현상,유전
Pre-eclampsia%Osteoprotegerin%Polymorphism,genetic
目的 探讨骨保护素基因启动子区163A/G和950T/C位点的多态性与重度子痫前期发病的关系.方法 选择2007年7月-2009年3月在四川大学华西第二医院就诊的成都市汉族孕妇共166例.其中重度子痫前期孕妇85例(重度子痫前期组),健康足月孕妇81例(对照组).应用PCR限制性片段长度多态性(RFLP)技术测定两组孕妇骨保护素基因启动子区163A/G和950T/C位点的基因型及等位基因频率,对两组中不同等位基因孕妇的血压、血肌酐、24 h尿蛋白定量、新生儿出生体质量等临床指标进行比较.结果(1)骨保护素基因启动子区163A/G、950T/C位点的基因型及等位基因频率在两组孕妇中的分布均符合Hardy-Weinberg遗传平衡定律.163A/G位点的基因型为AA、AG、GG,等位基因为A、G；950T/C位点的基因型为TT、TC、CC,等位基因为T、C.(2)重度子痫前期组孕妇163A/G、950T/C位点的基因型、等位基因频率与对照组比较,差异均无统计学意义(P＞0.05).(3)重度子痫前期组中163A/G位点AG+ GG基因型孕妇血肌酐水平[(76±24)μmol/L]明显高于AA基因型孕妇[(56±18)μmol/L],而新生儿出生体质量[(2040±721)g]显著低于AA基因型孕妇[(2520±810)g],两者比较,差异均有统计学意义(P＜0.05).对照组中163A/G位点的AG+ GG基因型孕妇血尿素、血肌酐、新生儿出生体质量、新生儿身长等临床指标与AA基因型孕妇比较,差异均无统计学意义(P＞0.05).(4)重度子痫前期组中950T/C位点的TT基因型孕妇收缩压[(153±16)mm Hg(1 mm Hg =0.133 kPa)]、24 h尿蛋白定量[(4.0±2.5)g]均显著高于TC+ CC基因型孕妇[分别为(145±17)mmHg及(2.9±1.8)g],两者比较,差异均有统计学意义(P＜0.05)；而对照组中950T/C位点的不同基因型孕妇各临床指标之间比较,差异均无统计学意义(P＞0.05).结论 携带163A/G位点的G等位基因孕妇比携带A等位基因者更具有重度子痫前期遗传易感性；携带950T/C位点的T等位基因孕妇比携带C等位基因者也更具有重度子痫前期遗传易感性.提示骨保护素基因多态性可能与重度子痫前期的发病有关.
目的 探討骨保護素基因啟動子區163A/G和950T/C位點的多態性與重度子癇前期髮病的關繫.方法 選擇2007年7月-2009年3月在四川大學華西第二醫院就診的成都市漢族孕婦共166例.其中重度子癇前期孕婦85例(重度子癇前期組),健康足月孕婦81例(對照組).應用PCR限製性片段長度多態性(RFLP)技術測定兩組孕婦骨保護素基因啟動子區163A/G和950T/C位點的基因型及等位基因頻率,對兩組中不同等位基因孕婦的血壓、血肌酐、24 h尿蛋白定量、新生兒齣生體質量等臨床指標進行比較.結果(1)骨保護素基因啟動子區163A/G、950T/C位點的基因型及等位基因頻率在兩組孕婦中的分佈均符閤Hardy-Weinberg遺傳平衡定律.163A/G位點的基因型為AA、AG、GG,等位基因為A、G；950T/C位點的基因型為TT、TC、CC,等位基因為T、C.(2)重度子癇前期組孕婦163A/G、950T/C位點的基因型、等位基因頻率與對照組比較,差異均無統計學意義(P＞0.05).(3)重度子癇前期組中163A/G位點AG+ GG基因型孕婦血肌酐水平[(76±24)μmol/L]明顯高于AA基因型孕婦[(56±18)μmol/L],而新生兒齣生體質量[(2040±721)g]顯著低于AA基因型孕婦[(2520±810)g],兩者比較,差異均有統計學意義(P＜0.05).對照組中163A/G位點的AG+ GG基因型孕婦血尿素、血肌酐、新生兒齣生體質量、新生兒身長等臨床指標與AA基因型孕婦比較,差異均無統計學意義(P＞0.05).(4)重度子癇前期組中950T/C位點的TT基因型孕婦收縮壓[(153±16)mm Hg(1 mm Hg =0.133 kPa)]、24 h尿蛋白定量[(4.0±2.5)g]均顯著高于TC+ CC基因型孕婦[分彆為(145±17)mmHg及(2.9±1.8)g],兩者比較,差異均有統計學意義(P＜0.05)；而對照組中950T/C位點的不同基因型孕婦各臨床指標之間比較,差異均無統計學意義(P＞0.05).結論 攜帶163A/G位點的G等位基因孕婦比攜帶A等位基因者更具有重度子癇前期遺傳易感性；攜帶950T/C位點的T等位基因孕婦比攜帶C等位基因者也更具有重度子癇前期遺傳易感性.提示骨保護素基因多態性可能與重度子癇前期的髮病有關.
목적 탐토골보호소기인계동자구163A/G화950T/C위점적다태성여중도자간전기발병적관계.방법 선택2007년7월-2009년3월재사천대학화서제이의원취진적성도시한족잉부공166례.기중중도자간전기잉부85례(중도자간전기조),건강족월잉부81례(대조조).응용PCR한제성편단장도다태성(RFLP)기술측정량조잉부골보호소기인계동자구163A/G화950T/C위점적기인형급등위기인빈솔,대량조중불동등위기인잉부적혈압、혈기항、24 h뇨단백정량、신생인출생체질량등림상지표진행비교.결과(1)골보호소기인계동자구163A/G、950T/C위점적기인형급등위기인빈솔재량조잉부중적분포균부합Hardy-Weinberg유전평형정률.163A/G위점적기인형위AA、AG、GG,등위기인위A、G；950T/C위점적기인형위TT、TC、CC,등위기인위T、C.(2)중도자간전기조잉부163A/G、950T/C위점적기인형、등위기인빈솔여대조조비교,차이균무통계학의의(P＞0.05).(3)중도자간전기조중163A/G위점AG+ GG기인형잉부혈기항수평[(76±24)μmol/L]명현고우AA기인형잉부[(56±18)μmol/L],이신생인출생체질량[(2040±721)g]현저저우AA기인형잉부[(2520±810)g],량자비교,차이균유통계학의의(P＜0.05).대조조중163A/G위점적AG+ GG기인형잉부혈뇨소、혈기항、신생인출생체질량、신생인신장등림상지표여AA기인형잉부비교,차이균무통계학의의(P＞0.05).(4)중도자간전기조중950T/C위점적TT기인형잉부수축압[(153±16)mm Hg(1 mm Hg =0.133 kPa)]、24 h뇨단백정량[(4.0±2.5)g]균현저고우TC+ CC기인형잉부[분별위(145±17)mmHg급(2.9±1.8)g],량자비교,차이균유통계학의의(P＜0.05)；이대조조중950T/C위점적불동기인형잉부각림상지표지간비교,차이균무통계학의의(P＞0.05).결론 휴대163A/G위점적G등위기인잉부비휴대A등위기인자경구유중도자간전기유전역감성；휴대950T/C위점적T등위기인잉부비휴대C등위기인자야경구유중도자간전기유전역감성.제시골보호소기인다태성가능여중도자간전기적발병유관.
Objective To investigate the potential association between 163A/G and 950T/C polymorphisms of osteoprotegerin(OPG)gene and severe pre-eclampsia.Methods Eighty-five severe preeclamptic patients and 81 normal term pregnant women(as control group)were recruited from the Department of Obstetrics and Gynecology,West China Second University Hospital,Sichuan University during the period from July 2007 to March 2009,and they were all Han population living in Chengdu,China.Genotype and allele frequencies of 163A/G and 950T/C were determined by the PCR-restriction fragment length polymorphism(RFLP)assay.Clinical and biochemical parameters for different alleles between the patients and controls were compared for statistical significance respectively,such as blood pressure,serum creatinine and 24-hour urine protein.Results The observed and expected genotype counts were consistent with Hardy-Weinberg equilibrium.No significant differences were found in the genotype and allele frequencies of 163A/G and 950T/C polymorphisms between the two groups(P ＞ 0.05).However,in the preeclamptic group,serum creatinine was significantly higher in women with the AG + GG genotypes [(76 ±24)μmol/L]compared with AA genotype[(56 ± 18)μmol/L].Reversely,birth weight was lower in the AG + GG genotypes[(2040 ± 721)g]than those in the AA genotype[(2520 ± 810)g],and the P ＜0.05,respectively.In the severe pre-eclampsia,950T/C TT genotype carriers exhibited significantly higher systolic blood pressure[(153 ± 16)mm Hg(1 mm Hg =0.133 kPa)]and 24-hour urine protein [(4.0±2.5)g]compared with TT + TC carriers[(145 ±17)mm Hg,(2.9±1.8)g],respectively,furthermore the P ＜ 0.05.Conclusions In severe pre-eclampsia,carriers with G allele at position 163A/G has more genetic predisposition than A allele carriers,as well as 950T/C T allele carriers compared with C carriers.Taken together,this study suggested that OPG gene polymorphisms might be associated with some clinical parameters of severe pre-eclampsia.
