CAJ | 학술논문

目的探讨单核细胞趋化蛋白-1(MCP-1)及其受体(CCR2)在结核性胸膜炎患者外周血和胸水中的表达情况.方法采用流式细胞术检测结核性胸膜炎患者外周血单个核细胞及胸水细胞中的CCR2;用ELISA法检测结核性胸膜炎患者血清及胸水中的MCP-1.结果结核性胸膜炎患者外周血中MCP-1和CCR2表达明显高于正常对照组[MCP-1:(340.8±220.8)与(9.0±3.8)ng/L,P＜0.01;CCR2:(18.2±10.1)%与(6.9±3.5)%,P＜0.05];外周血中MCP-1与CCR2两者呈正相关(r=0.227,P＜0.05),胸水中检测到MCP-1和CCR2表达.结论 MCP-1和CCR2在结核性胸膜炎患者外周血和胸水中的表达明显增加,是参与结核性胸膜炎发生发展的重要免疫分子.
목적 탐토단핵세포추화단백-1(MCP-1)급기수체(CCR2)재결핵성흉막염환자외주혈화흉수중적표체정황.방법 채용류식세포술검측결핵성흉막염환자외주혈단개핵세포급흉수세포중적CCR2;용ELISA법검측결핵성흉막염환자혈청급흉수중적MCP-1.결과 결핵성흉막염환자외주혈중MCP-1화CCR2표체명현고우정상대조조[MCP-1:(340.8±220.8)여(9.0±3.8)ng/L,P＜0.01;CCR2:(18.2±10.1)%여(6.9±3.5)%,P＜0.05];외주혈중MCP-1여CCR2량자정정상관(r=0.227,P＜0.05),흉수중검측도MCP-1화CCR2표체.결론 MCP-1화CCR2재결핵성흉막염환자외주혈화흉수중적표체명현증가,시삼여결핵성흉막염발생발전적중요면역분자.
Objective To investigate the expression of monocyte chemoattractant protein-1(MCP-1)and CCR2 in peripheral blood and pleural fluid of tuberculous pleurisy patients.Methods Flow cytometry was used to detect the expression of CCR2 in the peripheral blood mononuclear cell and pleural fluid cell of tuberculous pleurisy patients;ELISA was used to detect the content of MCP-1 in serum and pleural fluid.Results MCP-1 in surem and pleural fluid and CCR2 in the peripheral blood mononuclear cell and pleural fluid cell of tuberculous pleurisy patients was significantly higher than that of the healthy normal controls[MCP-1:(340.8±220.8)and(9.0±3.8)ng/L,P＜0.01;CCR2(18.2±10.1)%and(6.9±3.5)%,P＜0.05];Both MCP-1 and CCR2 were detected in pleural fluid and both of them were positivley correlated(r=0.227,P＜0.05).Conclusion The expression of MCP-1 and CCR2 in peripheral blood of tuberculous pleurisy patient are significantly elevated which are significance molecule participating in the pathogenesis of tuberculous pleurisy.