中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2012年
4期
346-348
,共3页
角膜%生物力学特性%应力-应变%松弛%蠕变
角膜%生物力學特性%應力-應變%鬆弛%蠕變
각막%생물역학특성%응력-응변%송이%연변
Cornea%Biomechanical property%Stress and Strain%Uniaxial tension%Creep
背景 研究证实软体生物组织在受力状态下具有稳定的生物力学特性.对于人的眼球来说,全面研究其生物力学特性较为困难,常常受到仪器设备、跨学科知识及材料获得等多因素的影响.兔是眼科学研究的主要动物模型之一,有关正常兔中央角膜的生物力学特性尚不清楚. 目的 了解兔角膜生物力学特性,获得正常兔角膜中央区的生物力学参数.方法 10只清洁级家兔处死后沿角膜缘分离完整的角膜,按照7 mm×5 mm的模具制备20个长方形角膜组织试件,在常温和空气加湿器加湿以模拟活体组织环境下,用BOSE electroforce3220-AT型生物力学试验机进行拉伸破坏试验、应力-应变试验、应力松弛试验和蠕变试验,得到角膜组织的拉伸破坏曲线、应力-应变关系曲线、应力松弛曲线和蠕变曲线,评估兔角膜组织的生物力学参数.结果 兔角膜的最大强度为(7.7432±0.6099) MPa,经过3次循环加载后,应力-应变曲线逐渐平稳.应力随着时间的延长逐渐衰减,前期衰减较快,随着时间的延长曲线趋于平缓,松弛率为30.33％；而角膜试件的形变随时间的延长而逐渐增大,早期形变较快,后期逐渐变缓,蠕变率为24.33％. 结论 揭示了兔角膜生物力学特性,为以后能否用兔眼模型研究人类眼部疾病提供力学指导.
揹景 研究證實軟體生物組織在受力狀態下具有穩定的生物力學特性.對于人的眼毬來說,全麵研究其生物力學特性較為睏難,常常受到儀器設備、跨學科知識及材料穫得等多因素的影響.兔是眼科學研究的主要動物模型之一,有關正常兔中央角膜的生物力學特性尚不清楚. 目的 瞭解兔角膜生物力學特性,穫得正常兔角膜中央區的生物力學參數.方法 10隻清潔級傢兔處死後沿角膜緣分離完整的角膜,按照7 mm×5 mm的模具製備20箇長方形角膜組織試件,在常溫和空氣加濕器加濕以模擬活體組織環境下,用BOSE electroforce3220-AT型生物力學試驗機進行拉伸破壞試驗、應力-應變試驗、應力鬆弛試驗和蠕變試驗,得到角膜組織的拉伸破壞麯線、應力-應變關繫麯線、應力鬆弛麯線和蠕變麯線,評估兔角膜組織的生物力學參數.結果 兔角膜的最大彊度為(7.7432±0.6099) MPa,經過3次循環加載後,應力-應變麯線逐漸平穩.應力隨著時間的延長逐漸衰減,前期衰減較快,隨著時間的延長麯線趨于平緩,鬆弛率為30.33％；而角膜試件的形變隨時間的延長而逐漸增大,早期形變較快,後期逐漸變緩,蠕變率為24.33％. 結論 揭示瞭兔角膜生物力學特性,為以後能否用兔眼模型研究人類眼部疾病提供力學指導.
배경 연구증실연체생물조직재수력상태하구유은정적생물역학특성.대우인적안구래설,전면연구기생물역학특성교위곤난,상상수도의기설비、과학과지식급재료획득등다인소적영향.토시안과학연구적주요동물모형지일,유관정상토중앙각막적생물역학특성상불청초. 목적 료해토각막생물역학특성,획득정상토각막중앙구적생물역학삼수.방법 10지청길급가토처사후연각막연분리완정적각막,안조7 mm×5 mm적모구제비20개장방형각막조직시건,재상온화공기가습기가습이모의활체조직배경하,용BOSE electroforce3220-AT형생물역학시험궤진행랍신파배시험、응력-응변시험、응력송이시험화연변시험,득도각막조직적랍신파배곡선、응력-응변관계곡선、응력송이곡선화연변곡선,평고토각막조직적생물역학삼수.결과 토각막적최대강도위(7.7432±0.6099) MPa,경과3차순배가재후,응력-응변곡선축점평은.응력수착시간적연장축점쇠감,전기쇠감교쾌,수착시간적연장곡선추우평완,송이솔위30.33％；이각막시건적형변수시간적연장이축점증대,조기형변교쾌,후기축점변완,연변솔위24.33％. 결론 게시료토각막생물역학특성,위이후능부용토안모형연구인류안부질병제공역학지도.
Background It has been proved that,after being forced,the biological soft tissue has stable biomechanical characteristics.However,there is rare study on corneal biomechanics.Rabbit is a main animal for experimental study in ophthalmology.But the biomechanical study of cornea in normal rabbit has not been reported.Objective This study was to investigate the biomechanical properties of normal rabbit central cornea and acquire the parameter. Methods Ten rabbits were sacrificed and the whole corneas were obtained and 20 central cornea specimens with 7 mm×5 mm of rabbit were prepared and tested on BOSE electroforce 3220-AT biomechanics machine under the room temperature and suitable humidity environment.Uniaxial tension,stress between strain,relaxation and creep were performed and the curves were drawn.The data was collected by wintest system to evaluate the biomechanical parameters of rabbit corneal tissue. Results The maximum distortion intension of rabbit cornea was (7.7432±0.6099)MPa.After three cyclic loading,the stress gradually attenuated and the stress and strain flattened as the time change with the relaxation rate 30.33％.The deformation of the specimens enhanced with time decrease with the creep rate 24.33％. Conclusions The biomechanical characteristics of normal rabbit cornea are revealed in this study,which offer the basis for the experimental research of rabbit model aimed at corneal disease.
