CAJ | 학술논문

目的探讨裸鼠Bel7402肝癌经腺病毒介导的胞嘧啶脱氨酶-脱氧胸苷激酶(Ad.CD-TK)双自杀基因治疗后的DWI特点,进而判定DWI是否可以用于肿瘤术后疗效评估.方法采用细胞悬液法成功制作Bel7402肝癌皮下移植瘤裸鼠模型30只,采用数宁表法随机抽取20只裸鼠作为治疗组,前3d每天瘤内注射Ad.CD-TK重组腺病毒,然后给予前体药5-氟胞嘧啶(5-Fc)和丙氧鸟苷(GCV)腹腔注射治疗；另10只裸鼠作为对照组不做治疗.两组鼠分别在治疗前、治疗后第10天行MR扫描,测量肿瘤ADC值,计算肿瘤体积,并采用t检验比较.MR扫描结束后立即取肿瘤标本,分别行HE染色和DNA缺口末端标记(TUNEL)检测,观察肿瘤细胞的坏死和凋亡情况,计算肿瘤细胞凋亡指数并采用t检验比较.结果治疗后10 d治疗组和对照组的肿瘤体积分别为(724.16±57.45)和(754.57±66.84) mm3,差异无统计学意义(t =0.488,P＞0.05).治疗后10d治疗组和对照组的肿瘤ADC值分别为(0.98±0.11) ×10-3和(0.68 ±0.04)×10-3mm2/s,肿瘤细胞凋亡指数分别是(23.25±6.57)％和(2.57±0.58)％,差异均有统计学意义(t值分别为4.473和5.874,P值均＜0.01).结论 DWI能有效反映Ad CD-TK双自杀基因治疗裸鼠Be17402肝癌的早期组织微观情况,为临床提供实验依据.
목적 탐토라서Bel7402간암경선병독개도적포밀정탈안매-탈양흉감격매(Ad.CD-TK)쌍자살기인치료후적DWI특점,진이판정DWI시부가이용우종류술후료효평고.방법 채용세포현액법성공제작Bel7402간암피하이식류라서모형30지,채용수저표법수궤추취20지라서작위치료조,전3d매천류내주사Ad.CD-TK중조선병독,연후급여전체약5-불포밀정(5-Fc)화병양조감(GCV)복강주사치료；령10지라서작위대조조불주치료.량조서분별재치료전、치료후제10천행MR소묘,측량종류ADC치,계산종류체적,병채용t검험비교.MR소묘결속후립즉취종류표본,분별행HE염색화DNA결구말단표기(TUNEL)검측,관찰종류세포적배사화조망정황,계산종류세포조망지수병채용t검험비교.결과 치료후10 d치료조화대조조적종류체적분별위(724.16±57.45)화(754.57±66.84) mm3,차이무통계학의의(t =0.488,P＞0.05).치료후10d치료조화대조조적종류ADC치분별위(0.98±0.11) ×10-3화(0.68 ±0.04)×10-3mm2/s,종류세포조망지수분별시(23.25±6.57)％화(2.57±0.58)％,차이균유통계학의의(t치분별위4.473화5.874,P치균＜0.01).결론 DWI능유효반영Ad CD-TK쌍자살기인치료라서Be17402간암적조기조직미관정황,위림상제공실험의거.
Objective To study the characteristics of DWI in nude mice models of hepatic Bel7402 tumors after treatment with adenovirus-mediated cytosine diaminase-thymidine kinase ( Ad.CD-TK) double suicide gene therapy, and then to identify whether DWI can be used for assessing curative effect of postoperative tumors.Methods Thirty nude mice models of hepatic Be17402 tumors were successfully created using cell suspension method,after the tumor grew to more than 1 cm in diameter,20 tumor models were treated by intratumoral administration of Ad.CD-TK for 3 days plus intraperitonea( i.p.) treatment with 5-Fc and GCV for the duration of the study.Then they were randomly divided into three groups during 5-Fc and GCV treatment.The remaining 10 tumor models were used as controls.MR scanning were performed in 10th day before and after tumor implantation in all models by using EPI-SE series and SENSE technology for treatment group. Tumor volumes and ADC values were calculated pretreatment and posttreatment. Cell apoptosis were determined by using TUNEL method.Analyze the change of ADC and apoptosis index (AI) in different times,t test was used for comparison the difference of AI and ADC values respectively. Results After 10 days,the tumor volumes of the treatment groups and controls were respectively (724.16 ±57.45 ) mm3,( 754.57 ± 66.84 ) mm3,with no significant difference ( t =0.488,P ＞ 0.05 ).The ADC values of the treatment groups were (0.98 ±0.11 ) × 10-3 mm2/s,the ones of the control groups were (0.68 ±0.04) × 10 -3mm2/s;AI of the treatment groups were(23.25 ±6.57)％,the ones of the control groups were (2.57 ± 0.58) ％.There were difference in both groups ( t =4.473,5.874 ; P ＜ 0.01 ).Conclusion DWI can be effectively to monitor the early pathological changes of hepatic Bel7402 tumors after Ad.CD-TK double suicide gene therapy,and provide experimental evidences for clinical application.