CAJ | 학술논문

目的观察平板跑步运动对大鼠关节软骨蛋白多糖的影响.方法将Wistar大鼠10只随机分为对照组和跑步组.对照组笼养,跑步组每天以20m/min速度,连续跑步1000m,每天1次.连续训练45d后,处死大鼠,切取双膝关节,固定,脱钙,包埋,沿矢状面整体切片,苏木素-伊红(HE)及番红O染色.观察股骨内侧髁负重区和胫骨内侧平台负重区软骨形态结构和基质染色差异,利用图像分析系统,测量胫骨平台软骨厚度,并对软骨各层染色深浅进行吸光度定量.结果对照组软骨结构正常,跑步组软骨表面完整,胫骨内侧平台负重区非钙化层厚度较对照组显著下降(P<0.05);番红O染色,负重区软骨非钙化层平均吸光度较对照组明显下降(P<0.05),其中表层下降66%(P<0.01),中层下降56%(P<0.01),深层与对照组差异无统计学意义(P>0.05),而钙化层吸光度较对照组上升38%(P<0.05).结论大鼠连续平板运动45d后,膝关节负重区软骨虽然结构完整,但已经出现退行性改变.
목적 관찰평판포보운동대대서관절연골단백다당적영향.방법 장Wistar대서10지수궤분위대조조화포보조.대조조롱양,포보조매천이20m/min속도,련속포보1000m,매천1차.련속훈련45d후,처사대서,절취쌍슬관절,고정,탈개,포매,연시상면정체절편,소목소-이홍(HE)급번홍O염색.관찰고골내측과부중구화경골내측평태부중구연골형태결구화기질염색차이,이용도상분석계통,측량경골평태연골후도,병대연골각층염색심천진행흡광도정량.결과 대조조연골결구정상,포보조연골표면완정,경골내측평태부중구비개화층후도교대조조현저하강(P<0.05);번홍O염색,부중구연골비개화층평균흡광도교대조조명현하강(P<0.05),기중표층하강66%(P<0.01),중층하강56%(P<0.01),심층여대조조차이무통계학의의(P>0.05),이개화층흡광도교대조조상승38%(P<0.05).결론 대서련속평판운동45d후,슬관절부중구연골수연결구완정,단이경출현퇴행성개변.
Objective To study the influences of the strenuous running training program on the knee joint articular cartilage. Methods At the age of 16 weeks, 5 male Wistar rats started running on a horizontal treadmill. Thereafter, the rats were trained for 45 days continuously. The rats ran 1 km/day at the speed of 20 m/min. Five matched male rats served as controls. Knees of the rats were studied by whole-mounted section, Hematoxylin and Eosin (HE) stain, safranin O stain. Results The cartilage surfaces were intact after the running exercise. The glycosaminoglycan concentration was decreased by an average of 66% in the superficial zone, 56% on the middle zone, but no difference was found in the deep zone. The glycosaminoglycan concentration was increased by 38% in the calcified cartilage. Conclusion Strenuous running induced marked decrease of proteoglycan in the uncalcified cartilage, but induced no osteoarthritis in this rat strenuous running model.