CAJ | 학술논문

目的了解浙江部分地区蚊媒及猪携带流行性乙型脑炎(乙脑)病毒情况以及浙江省存在的乙脑病毒基因型别.方法于2007年5月至10月在浙江省仙居、龙泉和慈溪3个县人房及猪舍采集蚊虫标本和猪血清,共采集10 662只蚊虫和204份猪血清,蚊虫标本中以淡色库蚊和中华按蚊为主.利用病毒分离和荧光定量逆转录聚合酶链反应(RT-PCR)方法检测蚊虫携带乙脑病毒,应用酶联接免疫吸附剂测定(ELISA)方法对猪血清进行抗体检测.应用荧光定量RT-PCR方法对细胞分离株进行病毒鉴定;利用RT-PCR方法扩增新分离乙脑病毒PrM基因,并对细胞分离到的病毒进行基因分型.结果在蚊虫标本中检出7批乙脑病毒核酸阳性样本,并分离出3株病毒,经鉴定3株病毒均为乙脑病毒,基因分析表明3株病毒均属于基因Ⅰ型乙脑病毒.猪血清有121份阳性,总阳性率为59.3%.6月份有50%以上的猪血清中乙脑病毒抗体呈阳性.结论浙江省部分地区存在着乙脑的传播媒介,在蚊媒和猪中携带有乙脑病毒;采集的蚊虫标本中分离到3株病毒,经鉴定为基因Ⅰ型乙脑病毒,是近年来在浙江省首次分离到的基因Ⅰ型乙脑病毒.
목적 료해절강부분지구문매급저휴대류행성을형뇌염(을뇌)병독정황이급절강성존재적을뇌병독기인형별.방법 우2007년5월지10월재절강성선거、룡천화자계3개현인방급저사채집문충표본화저혈청,공채집10 662지문충화204빈저혈청,문충표본중이담색고문화중화안문위주.이용병독분리화형광정량역전록취합매련반응(RT-PCR)방법검측문충휴대을뇌병독,응용매련접면역흡부제측정(ELISA)방법대저혈청진행항체검측.응용형광정량RT-PCR방법대세포분리주진행병독감정;이용RT-PCR방법확증신분리을뇌병독PrM기인,병대세포분리도적병독진행기인분형.결과 재문충표본중검출7비을뇌병독핵산양성양본,병분리출3주병독,경감정3주병독균위을뇌병독,기인분석표명3주병독균속우기인Ⅰ형을뇌병독.저혈청유121빈양성,총양성솔위59.3%.6월빈유50%이상적저혈청중을뇌병독항체정양성.결론 절강성부분지구존재착을뇌적전파매개,재문매화저중휴대유을뇌병독;채집적문충표본중분리도3주병독,경감정위기인Ⅰ형을뇌병독,시근년래재절강성수차분리도적기인Ⅰ형을뇌병독.
Objective To investigate the natural infection and genotype of Japanese encephalitis virus in mosquitoes and swine in some areas of Zhejiang province. Methods Samples of mosquitoes and sera of swine were collected in three counties (Xianju, Longquan and Cixi)of Zhejiang province from May to October in 2007.10 662 mosquitoes were collected during 2007, of which, C. pipiens pallens and C. quinquefasciatus were the most dominant species and 204 pig serum samples were detected. Japanese encephalitis virus in mosquitoes were detected by virus isolation and real time RT-PCR. The antibody against Japanese encephalitis virus in swine was detected by ELISA. The isolated strain were identified by real time RT-PCR. The PrM gene of the isolated strain was amplified by RT-PCR. Three strains were typed by the gene of PrM. Results Seven positive mosquitoe samples were identified by real time RT-PCR. Three strains were isolated and identified by real time RT-PCR. The PrM gene was cloned and sequenced. The phylogenetic analysis showed that three isolates belong to genotype Ⅰ of Japanese encephalitis virus. Of 204 swine serum samples, 121 positive samples were identified positives. Above 50% sera samples from swine were positive in June. Conclusion The vector of Japanese encephalitis virus existed and carried the Japanese encephalitis virus in these areas of Zhejiang province. Three strains of Japanese encephalitis virus were isolated from mosquito pools collected in Zhejiang province. It should be the first isolation of genotype Ⅰ Japanese encephalitis virus in Zhejiang province in recent years.