CAJ | 학술논문

报道了用基因枪转化法将雪花莲凝集素基因(gna)转移到优良籼型杂交稻恢复系蜀恢527中.PCR、PCR-Southern blotting和Southern blotting等分子检测证明,外源基因以多拷贝单位点方式整合到受体基因组中并稳定遗传到转基因第三代(T2).转基因第一代植株(T0)在株高和结实率上与相应的组培、种子实生苗植株相比,发生明显的不可遗传的变异,随着繁殖代数的增加,转基因植株恢复到与对照植株一致.Western blotting表明,目的基因在转基因植株中正确表达.蛋白活性分析显示,外源基因在转基因植株中合成的蛋白具有凝血生物活性,含量占可溶性总蛋白的0.1%左右.本文还对转基因技术在杂交稻育种中的应用进行了讨论. 图4 表2 参18
보도료용기인창전화법장설화련응집소기인(gna)전이도우량선형잡교도회복계촉회527중.PCR、PCR-Southern blotting화Southern blotting등분자검측증명,외원기인이다고패단위점방식정합도수체기인조중병은정유전도전기인제삼대(T2).전기인제일대식주(T0)재주고화결실솔상여상응적조배、충자실생묘식주상비,발생명현적불가유전적변이,수착번식대수적증가,전기인식주회복도여대조식주일치.Western blotting표명,목적기인재전기인식주중정학표체.단백활성분석현시,외원기인재전기인식주중합성적단백구유응혈생물활성,함량점가용성총단백적0.1%좌우.본문환대전기인기술재잡교도육충중적응용진행료토론. 도4 표2 삼18
This paper reports that the transgenic elite indica restorer line containing snowdrop lectin (Galanthus nivalis agglutinin, GNA) gene gna was obtained by biolistics and identified by PCR, PCR-Southern blotting and Southern blotting that the transgenes were integrated with estimated multi-copy at the same location of the genome of SH527 and transmitted stably from the first generation (T0) of transgenic plants to the third generation (T2). The plant height and seed-setting percentage of the first generation (T0) of transgenic plants were reduced apparently as compared with the corresponding plants derived from seeds and untransformed calli. However, this kind of variation could not be inherited stably. The protein analysis showed that the insect-resistant gene expressed in T0, T1 and T2 plants. The expressed protein had the biological activity and the ratio of GNA from transgenic plants to their total soluble protein was about 0.1%. The usage of genetic engineering in the breeding programs of hybrid rice was also discussed. Fig 4, Tab 2, Ref 18