国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2009年
10期
969-970,973
,共3页
王岐洁%熊亚星%段乃荣%肖萍%杨彦麟%魏喜生%陈青锋
王岐潔%熊亞星%段迺榮%肖萍%楊彥麟%魏喜生%陳青鋒
왕기길%웅아성%단내영%초평%양언린%위희생%진청봉
肝炎%乙型%聚合酶链反应%DNA
肝炎%乙型%聚閤酶鏈反應%DNA
간염%을형%취합매련반응%DNA
Hepatitis B%Polymerase Chain Reaction%DNA
目的 探讨慢性乙型肝炎患者血清HBV cccDNA与血清AST、ALT及血清HBV-DNA之间关系.方法 应用实时荧光定量聚合酶链反应(FQ-PCR)技术检测慢性乙型肝炎患者血清HBV cccDNA及HBV-DNA水平.结果 91例乙肝患者中血清ALT、AST异常的分别有79例、59例,检出HBV cccDNA阳性的分别有53例、41例,检出率分别为67.09%、69.49%,检出率与正常组相比有统计学意义(P=0.001＜0.05,P=0.015＜0.05);血清HBV cccDNA在HBV-DNA复制水平＜104 copies/mL时检出率为11.11%,HBV-DNA复制水平104～6 copies/mL时检出率为53.33%,HBV-DNA复制水平＞106 copies/mL时检出率为86.05%,以上不同的HBV-DNA复制水平血清HBV cccDNA检出率相比差异有统计学意义(P=0.000＜0.05).结论 血清HBV cccDNA检出率与血清HBV-DNA水平及血清ALT、AST水平有明显正相关性,血请HBV cccDNA的动态检测可以作为临床治疗及监测的一个重要参考指标.
目的 探討慢性乙型肝炎患者血清HBV cccDNA與血清AST、ALT及血清HBV-DNA之間關繫.方法 應用實時熒光定量聚閤酶鏈反應(FQ-PCR)技術檢測慢性乙型肝炎患者血清HBV cccDNA及HBV-DNA水平.結果 91例乙肝患者中血清ALT、AST異常的分彆有79例、59例,檢齣HBV cccDNA暘性的分彆有53例、41例,檢齣率分彆為67.09%、69.49%,檢齣率與正常組相比有統計學意義(P=0.001＜0.05,P=0.015＜0.05);血清HBV cccDNA在HBV-DNA複製水平＜104 copies/mL時檢齣率為11.11%,HBV-DNA複製水平104～6 copies/mL時檢齣率為53.33%,HBV-DNA複製水平＞106 copies/mL時檢齣率為86.05%,以上不同的HBV-DNA複製水平血清HBV cccDNA檢齣率相比差異有統計學意義(P=0.000＜0.05).結論 血清HBV cccDNA檢齣率與血清HBV-DNA水平及血清ALT、AST水平有明顯正相關性,血請HBV cccDNA的動態檢測可以作為臨床治療及鑑測的一箇重要參攷指標.
목적 탐토만성을형간염환자혈청HBV cccDNA여혈청AST、ALT급혈청HBV-DNA지간관계.방법 응용실시형광정량취합매련반응(FQ-PCR)기술검측만성을형간염환자혈청HBV cccDNA급HBV-DNA수평.결과 91례을간환자중혈청ALT、AST이상적분별유79례、59례,검출HBV cccDNA양성적분별유53례、41례,검출솔분별위67.09%、69.49%,검출솔여정상조상비유통계학의의(P=0.001＜0.05,P=0.015＜0.05);혈청HBV cccDNA재HBV-DNA복제수평＜104 copies/mL시검출솔위11.11%,HBV-DNA복제수평104～6 copies/mL시검출솔위53.33%,HBV-DNA복제수평＞106 copies/mL시검출솔위86.05%,이상불동적HBV-DNA복제수평혈청HBV cccDNA검출솔상비차이유통계학의의(P=0.000＜0.05).결론 혈청HBV cccDNA검출솔여혈청HBV-DNA수평급혈청ALT、AST수평유명현정상관성,혈청HBV cccDNA적동태검측가이작위림상치료급감측적일개중요삼고지표.
Objective To study the relationship between serum HBV covalently closed-circular DNA(cccDNA)and other related serum indicators(AST,ALT and HBV-DNA)in patients with chronic hepatitis B.Methods Real-time fluorescence quantitative polymerase chain reaction(FQ-PCR)was applied to measuring the serum levels of HBV cccDNA and DNA in 91 patients with chronic hepatitis B.Results In 91 cases of chronic hepatitis B,79 cases were ALT abnormal,in which 53 cases were HBV cccDNA positive(53/71,67.09%);59 cases were AST abnormal,in which 41 cases were HBV cccDNA positive(41/59,69.49%).There was statistical difference in detection rate of HBV cccDNA between hepatitis group and healthy control group(both P＜0.05).The detection rate of serum HBV cccDNA was closely associated with the replication level of HBV DNA(P＜0.05).When HBV-DNA level was less than 104 copies/mL,the detection rate of serum HBV cccDNA was 11.11%;104～106 copies/mL,53.33%;higher than 106 copies/mL,86.05%.Conclusion The detection rate of serum HBV cccDNA is positively correlated to serum levels of AST,ALT and HBV-DNA.Serum HBV cccDNA can be used as an important indicator of clinical treatment and therapeutic effect monitoring of chronic hepatitis B.
