CAJ | 학술논문

实验对青岛沿海10月的石莼以及5月的石莼和裙带菜的叶面附着细菌群落进行了Biolog碳源利用实验,结果表明不同海藻表面的细菌群落碳源利用有较大的差异,且同一种海藻不同的采集时间其细菌群落也有较大的差异.荧光原位杂交方法表明采用EUB-fluo探针比SYBR-Green染色的效果要好; γ-Proteobacteria在不同海藻表面占据了较高的比例;裙带表面观察到了一些特异性链珠状细菌;石莼表面的细菌形态展示了较高的多样性.从海藻表面分离的优势菌落中分离到1株细菌,16SrDNA测序表明该序列与已知的Pseudoalteromonas16S rDNA序列的相似性为99.9%.本研究为海洋无环境公害防污材料的研究提供了一些新的思路.
실험대청도연해10월적석순이급5월적석순화군대채적협면부착세균군락진행료Biolog탄원이용실험,결과표명불동해조표면적세균군락탄원이용유교대적차이,차동일충해조불동적채집시간기세균군락야유교대적차이.형광원위잡교방법표명채용EUB-fluo탐침비SYBR-Green염색적효과요호; γ-Proteobacteria재불동해조표면점거료교고적비례;군대표면관찰도료일사특이성련주상세균;석순표면적세균형태전시료교고적다양성.종해조표면분리적우세균락중분리도1주세균,16SrDNA측서표명해서렬여이지적Pseudoalteromonas16S rDNA서렬적상사성위99.9%.본연구위해양무배경공해방오재료적연구제공료일사신적사로.
AMicrobial communities were obtained from the surface of Ulva lactuca and Undaria pinnatifida in May 2008 and Ulva lactuca in October 2007. Biolog ecophysiological characteristic experiment revealed that the ecophysiology differences existed between both different algae species and different collection times. Fluorescence in situ hybridization observations suggested that the hybridization effect of probe EUB-fluo was much better than of Sybr Green; γ-Proteobacteria appeared to be dominant in the algae samples. Streptococcal bacteria were only detected from the surface of Undaria pinnatifida, while bacteria configurations showed more diversity on the surface of Ulva lactuca. One dominant bacteria strain was isolated from the algae based on the culture method, whose 16S rDNA sequence showed 99.9% similarity to the known Pseudoalteromonas species. The data gained supply a new idea and opinion for non-environmental harmful material in the future.