中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2010年
1期
41-45,50
,共6页
赵叶琳%赵晓寅%孙凌云%侯亚义
趙葉琳%趙曉寅%孫凌雲%侯亞義
조협림%조효인%손릉운%후아의
雌激素%间充质干细胞%树突状细胞%系统性红斑狼疮
雌激素%間充質榦細胞%樹突狀細胞%繫統性紅斑狼瘡
자격소%간충질간세포%수돌상세포%계통성홍반랑창
17-βEstradiol (E2)%Mesenchymal stem cells (MSC)%Dendritic cells (DC)%Systemic lupus erythematosus (SLE)
目的:本研究试图检测不同浓度17β雌二醇(F2)存在的情况下人胎肺间充质干细胞(MSC)增殖和表型的变化,并分析这种状态的MSC对树突状细胞(DC)成熟和功能影响及其可能的机制.方法:分离培养人胎肺MSC,加入不同浓度E2,在24小时后对MSC进行细胞计数、测定其增殖和贴壁能力,并用流式细胞仪分析MSC表面标志,RT-PCR测定MSC中细胞因子(IL-6、TGF-β和VEGF)mRNA的表达情况.进一步探讨了E2处理24小时后的MSC对DC成熟和功能的影响.结果:分离得到的MSC纯度达到95%以上;E2影响MSC的增殖和贴壁能力,但不影响MSC表面标记的表达;MSC与DC共培养后DC表面CD86、MHCⅡ和CD80的表达均有所降低,而当DC与经E2预处理过的MSC共培后,DC表面MHCⅡ、CD80和CD86的表达回升;高浓度E2作用MSC 24小时后,MSC表达的TGF-β与对照组相比减少,而IL-6和VEGF与对照组相比增加.结论:E2可能通过调节MSC分泌细胞因子的水平,改变MSC对DC的免疫抑制作用.
目的:本研究試圖檢測不同濃度17β雌二醇(F2)存在的情況下人胎肺間充質榦細胞(MSC)增殖和錶型的變化,併分析這種狀態的MSC對樹突狀細胞(DC)成熟和功能影響及其可能的機製.方法:分離培養人胎肺MSC,加入不同濃度E2,在24小時後對MSC進行細胞計數、測定其增殖和貼壁能力,併用流式細胞儀分析MSC錶麵標誌,RT-PCR測定MSC中細胞因子(IL-6、TGF-β和VEGF)mRNA的錶達情況.進一步探討瞭E2處理24小時後的MSC對DC成熟和功能的影響.結果:分離得到的MSC純度達到95%以上;E2影響MSC的增殖和貼壁能力,但不影響MSC錶麵標記的錶達;MSC與DC共培養後DC錶麵CD86、MHCⅡ和CD80的錶達均有所降低,而噹DC與經E2預處理過的MSC共培後,DC錶麵MHCⅡ、CD80和CD86的錶達迴升;高濃度E2作用MSC 24小時後,MSC錶達的TGF-β與對照組相比減少,而IL-6和VEGF與對照組相比增加.結論:E2可能通過調節MSC分泌細胞因子的水平,改變MSC對DC的免疫抑製作用.
목적:본연구시도검측불동농도17β자이순(F2)존재적정황하인태폐간충질간세포(MSC)증식화표형적변화,병분석저충상태적MSC대수돌상세포(DC)성숙화공능영향급기가능적궤제.방법:분리배양인태폐MSC,가입불동농도E2,재24소시후대MSC진행세포계수、측정기증식화첩벽능력,병용류식세포의분석MSC표면표지,RT-PCR측정MSC중세포인자(IL-6、TGF-β화VEGF)mRNA적표체정황.진일보탐토료E2처리24소시후적MSC대DC성숙화공능적영향.결과:분리득도적MSC순도체도95%이상;E2영향MSC적증식화첩벽능력,단불영향MSC표면표기적표체;MSC여DC공배양후DC표면CD86、MHCⅡ화CD80적표체균유소강저,이당DC여경E2예처리과적MSC공배후,DC표면MHCⅡ、CD80화CD86적표체회승;고농도E2작용MSC 24소시후,MSC표체적TGF-β여대조조상비감소,이IL-6화VEGF여대조조상비증가.결론:E2가능통과조절MSC분비세포인자적수평,개변MSC대DC적면역억제작용.
Objective:To investigate the effects of 17β-Estradiol (E2) on mesenchymal stem cells (MSC) and to evaluate the effects of MSC treated with E2 on the maturation and function of dendritic cells (DC).Methods: We first isolated and cultured MSC from the human fetal lung.The MSC were treated with E2 for 24 hours at various concentrations ( 10~(-9),10~(-8) and 10~(-7) mol/L).After cell counting,proliferation,adherent ability and immunophenotypes of MSC were detected by flowcytometry.The gene expressions of cytokine (IL-6,TGF-βand VEGF) were measured by RT-PCR.The effects of MSC treated with E2 on the maturation and function of DC were determined.Results:After treated with E2,the proliferation and adherent ability of MSC were increased,while the immunophenotypes of MSC were not affected.When MSCs co-cultured with DC,MSC could inhibit the immuophenotypes and function of DC.However,when DC co-cultured with E2-pretreated MSC,the immunophenotypes (MHC Ⅱ,CD80 and CD86) of DC had been reconstructed.After treated with the high concentration of E2 for 24 hours,MSC secreted lower level of TGF-β than that in the control group,while IL-6 and VEGF expressions were increased compared with those in the control group.Conclusion: Estrogen may alter the immuno-suppressive effects of MSC on DC via modulating the cytokine secretion of MSC.