CAJ | 학술논문

通过醋酸棉酚YPG培养基多级逐步驯化筛选,结合棉粕固态生物发酵的方法,筛选出1株高效降解棉酚的热带假丝酵母JD-9.通过单因素试验初步确定了棉粕发酵脱毒的工艺条件,进一步利用分式析因试验设计法,对影响棉粕脱毒率的因素进行了筛选,方差分析表明,影响棉粕发酵脱毒的主要因素为热带假丝酵母JD-9接种量和卡氏酵母JD-13接种量.通过响应面分析法和典型性分析得出棉粕发酵脱毒的最优条件为:热带假丝酵母JD-9接种量4.42%,卡氏酵母JD-13接种量0.56%,发酵时间48 h,发酵温度30 ℃,料水比1: 0.8,棉粕中的棉酚从987.5 mg/kg降解至85.9 mg/kg,脱毒率达到91.3%.
통과작산면분YPG배양기다급축보순화사선,결합면박고태생물발효적방법,사선출1주고효강해면분적열대가사효모JD-9.통과단인소시험초보학정료면박발효탈독적공예조건,진일보이용분식석인시험설계법,대영향면박탈독솔적인소진행료사선,방차분석표명,영향면박발효탈독적주요인소위열대가사효모JD-9접충량화잡씨효모JD-13접충량.통과향응면분석법화전형성분석득출면박발효탈독적최우조건위:열대가사효모JD-9접충량4.42%,잡씨효모JD-13접충량0.56%,발효시간48 h,발효온도30 ℃,료수비1: 0.8,면박중적면분종987.5 mg/kg강해지85.9 mg/kg,탈독솔체도91.3%.
The YPG medium containing acetic gossypol was used for the gossypol-degrading strain screening.A strain of Candida tropicalis JD-9 with high efficiency of gossypol-degrading was screened out.The methods of single factor experiments,fractional factorial experimental design and central composite experimental design were used to optimize the process parameters for detoxification of gossypol in cottonseed meal by Candida tropicalis JD-9 during solid substrate fermentation.The optimum fermentation conditions were as follows:inoculums level of JD-9(Candida tropicalis)4.42%,inoculums level of JD-13(S.carlsbergensis)0.56%,incubation time 48 h,incubation temperature 30 ℃,ratio of material to water 1:0.8.Under the optimum conditions,the content of gossypol reduced from 987.5 mg/kg to 85.9 mg/kg,and the detoxification efficiency was up to 91.3%.