CAJ | 학술논문

目的研发基于油镜的全自动显微图像扫描采集系统,用以替代传统的人工镜检读片,提高疟疾镜检效率和疟原虫检出率.方法基于油镜的全自动显微图像扫描采集系统由光学显微镜、数码摄像机/模拟摄像机、控制软件和普通计算机组成.采用盲法,用基于油镜的全自动显微图像扫描采集系统和传统镜下读片,各检读10张血片样本,每张血片读100个视野,并将读得的阳性血片计数.记录每个镜检员检读每张血片样本的时间,并计算读片准确率、读片速度、阳性血片和阴性血片的符合率.结果该系统目前实现的基本功能为自动位移和对焦,自动扫描和储存,自动回位和复位功能,标注和自动计数,报告单打印等.系统在疟疾诊断中初步应用效果:该系统较传统读片速度提高30.58%,读片的总符合率提高13.33个百分点.结论该系统在疟疾血片读片速度和准确度方面均优于传统镜下读片,且该系统操作简单、价格便宜、易于推广.
목적 연발기우유경적전자동현미도상소묘채집계통,용이체대전통적인공경검독편,제고학질경검효솔화학원충검출솔.방법 기우유경적전자동현미도상소묘채집계통유광학현미경、수마섭상궤/모의섭상궤、공제연건화보통계산궤조성.채용맹법,용기우유경적전자동현미도상소묘채집계통화전통경하독편,각검독10장혈편양본,매장혈편독100개시야,병장독득적양성혈편계수.기록매개경검원검독매장혈편양본적시간,병계산독편준학솔、독편속도、양성혈편화음성혈편적부합솔.결과 해계통목전실현적기본공능위자동위이화대초,자동소묘화저존,자동회위화복위공능,표주화자동계수,보고단타인등.계통재학질진단중초보응용효과:해계통교전통독편속도제고30.58%,독편적총부합솔제고13.33개백분점.결론 해계통재학질혈편독편속도화준학도방면균우우전통경하독편,차해계통조작간단、개격편의、역우추엄.
Objective To establish an automatic microscope scanning system based on the oil-lens for replacing the traditional manual microscopy reading of blood films to improve the efficiency and the detection rate of Plasmodium.Methods The system consisted of an optical microscope,digital camera,control software and general computer-based component.The system and professional persons read the blood films with single-blind method,everyone read 10 blood samples(100 fields of vision per blood film),and the time and results of reading were recorded.Results The system had the function of automatic displacement and focus,automatic scanning and storage,automatic back-bit and reset,annotation,and automatic counting,reporting and printing.The system can increased the speed of reading films by 30.58%,and improved the accuracy by 13.33%.Conclusion The automatic microscope scanning system can improve the speed and accuracy of reading films and the operation is simple.