CAJ | 학술논문

该文围绕目前工业化生产11S和7S组分的分离技术成果,结合实验室的分离技术(Guo法),比较了各种分离方法的差异.结果表明:分离提取技术利用的原理几乎均建立在"碱溶酸提"和"冷沉"作用基础之上.Wu首次成功地进行了7S和11S的工业化分离,之后的研究多是对此方法的改进和修饰.而Guo法是唯一采用中性条件抽提的工业化分离方法,在节省了原料碱的同时,避免了蛋白的变性.同时,该文提出了目前工业化生产大豆组分蛋白中存在的问题,虽然不断在加工方式上进行改进,但仍存在步骤复杂、产率低、生产成本高等方面的问题,因此作者进一步对实现工业化分离大豆组分蛋白提出了需要改进和努力的方向.
해문위요목전공업화생산11S화7S조분적분리기술성과,결합실험실적분리기술(Guo법),비교료각충분리방법적차이.결과표명:분리제취기술이용적원리궤호균건립재"감용산제"화"랭침"작용기출지상.Wu수차성공지진행료7S화11S적공업화분리,지후적연구다시대차방법적개진화수식.이Guo법시유일채용중성조건추제적공업화분리방법,재절성료원료감적동시,피면료단백적변성.동시,해문제출료목전공업화생산대두조분단백중존재적문제,수연불단재가공방식상진행개진,단잉존재보취복잡、산솔저、생산성본고등방면적문제,인차작자진일보대실현공업화분리대두조분단백제출료수요개진화노력적방향.
Combining with our isolation techniques (method of Guo), this article compared different separation methods of 11S and 7S fractions with industrial methods. The principles of most separation techniques are "alkali extraction and acid precipitation" and "cold precipitation" effects. Wu's method succeeded in isolation 11S and 7S fraction for the first time, the later researches mostly focus on its improvement. The method of Guo is the only method to extract protein in a neutral condition, thus saved the usage of alkali and avoided the denaturation of protein. Meanwhile, this article pointed out the problems in the current industrial production. Although lots of improvements were made on the separation process, problem still exist, such as complicated procedures, low yields, high costs of production and so on. Therefore, the writer further proposed the directions of efforts and improvement for the realization of industrial production.