CAJ | 학술논문

目的观察茶氨酸在体内外对肺癌A549细胞株生长的抑制、凋亡效应、抗肿瘤生成及抗血管生成作用.方法观察茶氨酸对肺癌A549细胞生长的影响,通过FCM检测茶氨酸对肺癌细胞凋亡的作用,以及茶氨酸在裸鼠移植瘤模型上对瘤体生长的抑制作用,免疫组化法检测肿瘤组织中CD34和血管内皮生长因子(VEGF)表达,观察茶氨酸对肺癌生长的抑制作用.结果茶氨酸显著抑制A549肺癌细胞生长,具有时间依赖性和浓度依赖性.荼氨酸使A549细胞出现明显凋亡,凋亡率17.8%.茶氨酸组治疗2周后,移植瘤瘤体体积变化和瘤体重量变化差异均有统计学意义,抑瘤率为47.2%.茶氨酸组瘤体组织中VEGF的表达与PBS组比较差异有统计学意义,瘤体组织中CD34标记的微血管密度(MVD)降低,因此,茶氨酸显著抑制肿瘤血管形成.结论茶氨酸通过促进肿瘤细胞凋亡、抑制肿瘤生长及抑制肿瘤血管生成的作用.
목적 관찰다안산재체내외대폐암A549세포주생장적억제、조망효응、항종류생성급항혈관생성작용.방법 관찰다안산대폐암A549세포생장적영향,통과FCM검측다안산대폐암세포조망적작용,이급다안산재라서이식류모형상대류체생장적억제작용,면역조화법검측종류조직중CD34화혈관내피생장인자(VEGF)표체,관찰다안산대폐암생장적억제작용.결과 다안산현저억제A549폐암세포생장,구유시간의뢰성화농도의뢰성.도안산사A549세포출현명현조망,조망솔17.8%.다안산조치료2주후,이식류류체체적변화화류체중량변화차이균유통계학의의,억류솔위47.2%.다안산조류체조직중VEGF적표체여PBS조비교차이유통계학의의,류체조직중CD34표기적미혈관밀도(MVD)강저,인차,다안산현저억제종류혈관형성.결론 다안산통과촉진종류세포조망、억제종류생장급억제종류혈관생성적작용.
Objective To observe the effect of theanine in vitro and in vivo, including suppression of lung tumor growth, tumor an-giogenesis and promotion of apoptosis. Methods The inhibitory effects of theanie on lung cancer A549 cells were analyzed by MTT assays. The cell cycle and the apeptotic percentage of A549 cells were detected by FCM. The angiogenesis effect of theanine was observed with CAM model. The inhibitory effects of theanine were observed with lung carcinoma nude mice model, and the immunohistochemic technique was used to investigate the expression of CD34 and VEGF (vascular endothelial growth factor). Results Treatment of A549 cells with VEGF re-sulted in significant dose-dependent and time-dependent inhibition. FCM detection indicated that administration of EGCG resulted in an in-crease in cells in the S phase of the cell cycle and a typical apoptosis peak before the GI phase with an apoptosis rate of 15.9%. There was a significant difference in tumor volume and weight in theanine group compared with control group after two-week treatment, and the tumor in-hibition rate was 43.6%. There was no significant difference in expression of VEGF in tumor tissue according to tumor MVD marked by CD34 between the theanie group and control group. Thus, theanine could obviously inhibit tumor angiogenesis. Conclusion VEGF can ar-rest lung tumor growth via the promotion of tumor cell apoptosis and the inhibition of tumor angiogenesis.