CAJ | 학술논문

目的通过观察微弧氧化-碱热处理后钛铌锆锡合金(Ti-24Nb-4Zr-8Sn)表面成骨细胞的生长情况和种植体-骨界面的结合情况,探讨对钛铌锆锡合金表面应用微弧氧化-碱热处理进行改性的效果.方法用钛铌锆锡合金制备圆片形试件和圆柱形种植体各12个,均分为3个组(空白对照组、微弧氧化组和微弧氧化-碱热处理组)进行相应表面处理.将MC3T3-E1成骨样细胞接种于圆片形试件表面,培养3 d后扫描电镜(SEM)比较各组试件表面的细胞形态.将圆柱形种植体植入犬胫骨内,3个月后SEM观察种植体-骨界面的微观形貌.结果微弧氧化和微弧氧化-碱热处理均可使钛铌锆锡合金表面生成具有生成微孔结构的氧化膜,后者试件表面的细胞与微孔嵌合更紧密,种植体与骨组织形成良好的骨结合.结论碱热处理可进一步完善微弧氧化方法,提高种植体与骨界面的结合.
목적 통과관찰미호양화-감열처리후태니고석합금(Ti-24Nb-4Zr-8Sn)표면성골세포적생장정황화충식체-골계면적결합정황,탐토대태니고석합금표면응용미호양화-감열처리진행개성적효과.방법 용태니고석합금제비원편형시건화원주형충식체각12개,균분위3개조(공백대조조、미호양화조화미호양화-감열처리조)진행상응표면처리.장MC3T3-E1성골양세포접충우원편형시건표면,배양3 d후소묘전경(SEM)비교각조시건표면적세포형태.장원주형충식체식입견경골내,3개월후SEM관찰충식체-골계면적미관형모.결과 미호양화화미호양화-감열처리균가사태니고석합금표면생성구유생성미공결구적양화막,후자시건표면적세포여미공감합경긴밀,충식체여골조직형성량호적골결합.결론 감열처리가진일보완선미호양화방법,제고충식체여골계면적결합.
Objective To observe the efficacy of micro-arc oxidation and alkali-heat treatment (MAH) on Ti-24Nb-4Zr-8Sn(Ti2448). Methods Disks( diameter of 14. 5 mm, thickness of 1 mm) and cylinders (diameter of 3 mm, height of 10 mm) were fabricated from Ti2448 alloy. Samples were divided into three groups: polished( Ti2448 ), micro-arc oxidation( MAO-Ti2448 ), micro-arc oxidation and alkali-heat treatment(MAH-Ti2448). MC3T3-E1 osteoblastic cells were cultured on the disks and cell morphology was observed with scanning electron microscopy(SEM) aftre 3 days. The cylinder samples were implanted in the tibia of dogs and implant-bone interface was observed with SEM after 3 months. Results A rough and porous structure was shown in both MAO and MAH group. The MC3T3-E1 cells on the MAH-Ti2448 discs spread fully in intimate contact with the underlying coarse surface through active cytoskeletal extentions.Osseointegration was formed in the implant-bone interface in MAH samples. Conclusions MAH treatment can provide a more advantageous Ti2448 surface to osteoblastic cells than MAO treatment does, and the former can improve the implant-bone integration.