CAJ | 학술논문

目的观察小分子干扰RNA(siRNA)对人肾癌细胞Survivin基因表达及其增殖、凋亡的影响.方法设计、合成1对Survivin编码基因序列特异的siRNA,用脂质体包裹转染人肾癌786-O细胞,分不同浓度组(10、50、100 nmol/L),采用逆转录.聚合酶链反应(RT-PCR)、Western blot技术检测Survivin mRNA及蛋白表达,噻唑蓝(MTT)比色法检测细胞增殖,免疫组织化学TUNEL法榆测细胞凋亡.结果 Survivin siRNA能有效下调Survivin基因表达水平,抑制了细胞生长,促进其凋亡.并呈剂量依赖性(3组的mRNA 88.3%、62.4%、43.8%,蛋白87.7%、62.4%、46.5%,增殖抑制率11.6%、41.2%、57.5%,凋亡率14.2%、29.4%、38.1%),差异有统计学意义(P<0.05).结论 Survivin基因siRNA能抑制人肾癌786-O细胞Survivin基因表达,进而抑制其增殖,促进其凋亡.
목적 관찰소분자간우RNA(siRNA)대인신암세포Survivin기인표체급기증식、조망적영향.방법 설계、합성1대Survivin편마기인서렬특이적siRNA,용지질체포과전염인신암786-O세포,분불동농도조(10、50、100 nmol/L),채용역전록.취합매련반응(RT-PCR)、Western blot기술검측Survivin mRNA급단백표체,새서람(MTT)비색법검측세포증식,면역조직화학TUNEL법유측세포조망.결과 Survivin siRNA능유효하조Survivin기인표체수평,억제료세포생장,촉진기조망.병정제량의뢰성(3조적mRNA 88.3%、62.4%、43.8%,단백87.7%、62.4%、46.5%,증식억제솔11.6%、41.2%、57.5%,조망솔14.2%、29.4%、38.1%),차이유통계학의의(P<0.05).결론 Survivin기인siRNA능억제인신암786-O세포Survivin기인표체,진이억제기증식,촉진기조망.
Objective To evaluate the effects of small interfering RNA (siRNA) against Survivin gene on the proliferation and apoptosis of human renal carcinoma cell line 786-O cells. Methods One pair of Survivin target sequence-specific siRNA was designed and synthesized, then siRNA/liposome complex was used to transfect the human renal carcinoma 786-O cells with increasing concentrations (10, 50, 100 nmol/L). The mRNA and protein expression of Survivin was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively. The proliferation of 786-O cells was measured by MTT assay. The apoptosis of 786-O cells was examined by TUNEL assay. Results SiRNA-Survivin efficiently down-regulated the Survivin expression, inhibited the proliferation and induced the apoptosis in a dose dependent manner (for mRNA 88. 3% , 62.4% , 43. 8% , for protein 87. 7% , 62.4% , 46.5%, proliferation-inhibiting rate 11.6%, 41.2%, 57.5%, and apoptosis rate 14.2%, 29.4%, 38. 1% , respectively) (P<0.05). Conclusion siRNA against Survivin gene can inhibit the proliferation, respectively) (P<0.05). Conclusion siRNA against Survivin gene can inhibit the proliferation