CAJ | 학술논문

目的探讨血栓素A2(TXA2)合成酶抑制剂奥扎格雷钠对低氧肺动脉平滑肌细胞(PASMC)增殖及凋亡的影响.方法采用四甲基偶氮唑盐微量比色法、免疫组化法及原位末端脱氧核苷酸转移酶标记法观察TXA2激动剂U46619及TXA2合成酶抑制剂奥扎格雷钠对PASMC增殖及凋亡和对反映细胞增殖状态的重要标记物Ki67表达的影响.结果 U46619对常氧和低氧PASMC的增殖和低氧PASMC中Ki67的表达均有明显促进作用,并可明显抑制PASMC的凋亡;奥扎格雷钠对常氧PASMC的增殖无明显影响,但对低氧PASMC的增殖和Ki67的表达均有明显抑制作用,可促进低氧PASMC的凋亡;奥扎格雷钠可明显拮抗U46619在常氧和低氧条件下对PASMC的促增殖和Ki67表达的作用,以及U46619抑制低氧PASMC的凋亡的作用.结论奥扎格雷钠有可能通过抑制低氧PASMC的增殖及血管重塑、促进凋亡,从而对低氧性肺动脉高压起治疗作用.
목적 탐토혈전소A2(TXA2)합성매억제제오찰격뢰납대저양폐동맥평활기세포(PASMC)증식급조망적영향.방법 채용사갑기우담서염미량비색법、면역조화법급원위말단탈양핵감산전이매표기법관찰TXA2격동제U46619급TXA2합성매억제제오찰격뢰납대PASMC증식급조망화대반영세포증식상태적중요표기물Ki67표체적영향.결과 U46619대상양화저양PASMC적증식화저양PASMC중Ki67적표체균유명현촉진작용,병가명현억제PASMC적조망;오찰격뢰납대상양PASMC적증식무명현영향,단대저양PASMC적증식화Ki67적표체균유명현억제작용,가촉진저양PASMC적조망;오찰격뢰납가명현길항U46619재상양화저양조건하대PASMC적촉증식화Ki67표체적작용,이급U46619억제저양PASMC적조망적작용.결론 오찰격뢰납유가능통과억제저양PASMC적증식급혈관중소、촉진조망,종이대저양성폐동맥고압기치료작용.
Objective To study the effect of Thromboxane A2 (TXA2) inhibitor on the proliferation of hypoxic pulmonary artery smooth muscle cells (PASMC) of porcines. Methods The methods of 3(4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide ( MTT), immunohistochemistry of Ki67 and TdTmediated dUTP nick end labeling(TUNEL) were employed to measure the proliferation, inhibition rate and apoptosis of PASMC. Results U46619, a thromboxane A2 agonist, could promote the proliferation and expression of Ki67 in PASMC under hypoxic and normal conditions. It could also inhibit the apoptosis of PASMC. Ozagrel, a thromboxane A2 inhibiter, inhibited the proliferation and the expression of Ki67 in PASMC under hypoxia. The inhibition rate was 71.4%, but it had no effect on the proliferation and expression of Ki67 in PASMC under normal conditions. It promoted the apoptosis of PASMC. Ozagrel could inhibit the action of U46619 in promoting the proliferation and expression of Ki67 in PASMC and inhibit the apoptosis of PASMC. Conclusion Ozagrel may be used in treating the pulmonary hypertension by inhibiting the proliferation of PASMC, pulmonary vascular remodeling, and promote the apoptosis of PASMC.