中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2006年
18期
184-186
,共3页
赵兰峰%李菁锦%安仰原%王毅珍
趙蘭峰%李菁錦%安仰原%王毅珍
조란봉%리정금%안앙원%왕의진
癫痫%迷走神经%电刺激%大脑皮质
癲癇%迷走神經%電刺激%大腦皮質
전간%미주신경%전자격%대뇌피질
背景:迷走神经刺激术是一种治疗难治性癫痫的神经生理学疗法,通过刺激颈部迷走神经干可达到控制癫痫发作的目的.目的:观察间断性左侧迷走神经刺激术对致痫动物痫性发作的影响,为躯体内脏信息相互作用提供理论根据.设计:观察性实验.单位:首都医科大学神经生物学系.材料:实验于2000-03/2002-09在首都医科大学神经生物学系电生理学实验室完成.实验选用健康成年SD大鼠34只,体质量220～250 g;健康成年家兔8只,体质量2.2～2.5 kg.方法:①10只大鼠经肌肉注射150～160万单位青霉素致痫,通过观察迷走神经刺激术前后大鼠大脑皮质电图、行为学的变化,研究迷走神经刺激术对致痫大鼠癫痫活动的影响.②另外8只大鼠向海马内注入青霉素0.24～0.48 mg致痫,观察迷走神经刺激术对致痫大鼠痫性大脑皮质电图的影响.③16只大鼠皮下注射海人藻酸致痫.经迷走神经刺激术观察致痫大鼠海马神经元放电活动、大脑皮质电图及行为学变化.④8只家兔用微量注射器向皮层滴注士的宁致痫,观察迷走神经刺激术对急性皮层损伤致痫家兔的大脑皮质电图的影响.主要观察指标:①迷走神经刺激术对青霉素致痫大鼠痫性发作的影响.②迷走神经刺激术对海人藻酸致痫的大鼠痫性发作的影响.③迷走神经刺激对士的宁致痫家兔痫性大脑皮质电图的影响.结果:34只大鼠,8只家兔均进入结果分析.迷走神经刺激可以阻抑各组致痫动物的痫性发作,痫性皮质电图、海马神经元电活动及行为学表现均呈现显著的变化,总有效率达50%以上.如在癫痫发作前先行迷走神经刺激,有效率可达80%以上.肌肉注射青霉素致痫组,行为学及大脑皮质电图明显改善分别为40%和50%.海马内注射青霉素致痫组,50%的大鼠大脑皮质电图明显改善.迷走神经刺激对海人藻酸致痫大鼠的痫性发作的有效控制率为80%.家兔大脑皮质局部滴注士的宁组,经迷走神经刺激,50%的大脑皮质电图的痫性波得到控制.结论:迷走神经刺激可有效地阻抑致痫动物的痫性发作,脑的皮质、海马神经元参与了迷走神经刺激的抗痫作用.内脏传入信息可能通过在脑的皮质、海马部位的整合作用达到有效地阻抑躯体痫性活动.
揹景:迷走神經刺激術是一種治療難治性癲癇的神經生理學療法,通過刺激頸部迷走神經榦可達到控製癲癇髮作的目的.目的:觀察間斷性左側迷走神經刺激術對緻癇動物癇性髮作的影響,為軀體內髒信息相互作用提供理論根據.設計:觀察性實驗.單位:首都醫科大學神經生物學繫.材料:實驗于2000-03/2002-09在首都醫科大學神經生物學繫電生理學實驗室完成.實驗選用健康成年SD大鼠34隻,體質量220～250 g;健康成年傢兔8隻,體質量2.2～2.5 kg.方法:①10隻大鼠經肌肉註射150～160萬單位青黴素緻癇,通過觀察迷走神經刺激術前後大鼠大腦皮質電圖、行為學的變化,研究迷走神經刺激術對緻癇大鼠癲癇活動的影響.②另外8隻大鼠嚮海馬內註入青黴素0.24～0.48 mg緻癇,觀察迷走神經刺激術對緻癇大鼠癇性大腦皮質電圖的影響.③16隻大鼠皮下註射海人藻痠緻癇.經迷走神經刺激術觀察緻癇大鼠海馬神經元放電活動、大腦皮質電圖及行為學變化.④8隻傢兔用微量註射器嚮皮層滴註士的寧緻癇,觀察迷走神經刺激術對急性皮層損傷緻癇傢兔的大腦皮質電圖的影響.主要觀察指標:①迷走神經刺激術對青黴素緻癇大鼠癇性髮作的影響.②迷走神經刺激術對海人藻痠緻癇的大鼠癇性髮作的影響.③迷走神經刺激對士的寧緻癇傢兔癇性大腦皮質電圖的影響.結果:34隻大鼠,8隻傢兔均進入結果分析.迷走神經刺激可以阻抑各組緻癇動物的癇性髮作,癇性皮質電圖、海馬神經元電活動及行為學錶現均呈現顯著的變化,總有效率達50%以上.如在癲癇髮作前先行迷走神經刺激,有效率可達80%以上.肌肉註射青黴素緻癇組,行為學及大腦皮質電圖明顯改善分彆為40%和50%.海馬內註射青黴素緻癇組,50%的大鼠大腦皮質電圖明顯改善.迷走神經刺激對海人藻痠緻癇大鼠的癇性髮作的有效控製率為80%.傢兔大腦皮質跼部滴註士的寧組,經迷走神經刺激,50%的大腦皮質電圖的癇性波得到控製.結論:迷走神經刺激可有效地阻抑緻癇動物的癇性髮作,腦的皮質、海馬神經元參與瞭迷走神經刺激的抗癇作用.內髒傳入信息可能通過在腦的皮質、海馬部位的整閤作用達到有效地阻抑軀體癇性活動.
배경:미주신경자격술시일충치료난치성전간적신경생이학요법,통과자격경부미주신경간가체도공제전간발작적목적.목적:관찰간단성좌측미주신경자격술대치간동물간성발작적영향,위구체내장신식상호작용제공이론근거.설계:관찰성실험.단위:수도의과대학신경생물학계.재료:실험우2000-03/2002-09재수도의과대학신경생물학계전생이학실험실완성.실험선용건강성년SD대서34지,체질량220～250 g;건강성년가토8지,체질량2.2～2.5 kg.방법:①10지대서경기육주사150～160만단위청매소치간,통과관찰미주신경자격술전후대서대뇌피질전도、행위학적변화,연구미주신경자격술대치간대서전간활동적영향.②령외8지대서향해마내주입청매소0.24～0.48 mg치간,관찰미주신경자격술대치간대서간성대뇌피질전도적영향.③16지대서피하주사해인조산치간.경미주신경자격술관찰치간대서해마신경원방전활동、대뇌피질전도급행위학변화.④8지가토용미량주사기향피층적주사적저치간,관찰미주신경자격술대급성피층손상치간가토적대뇌피질전도적영향.주요관찰지표:①미주신경자격술대청매소치간대서간성발작적영향.②미주신경자격술대해인조산치간적대서간성발작적영향.③미주신경자격대사적저치간가토간성대뇌피질전도적영향.결과:34지대서,8지가토균진입결과분석.미주신경자격가이조억각조치간동물적간성발작,간성피질전도、해마신경원전활동급행위학표현균정현현저적변화,총유효솔체50%이상.여재전간발작전선행미주신경자격,유효솔가체80%이상.기육주사청매소치간조,행위학급대뇌피질전도명현개선분별위40%화50%.해마내주사청매소치간조,50%적대서대뇌피질전도명현개선.미주신경자격대해인조산치간대서적간성발작적유효공제솔위80%.가토대뇌피질국부적주사적저조,경미주신경자격,50%적대뇌피질전도적간성파득도공제.결론:미주신경자격가유효지조억치간동물적간성발작,뇌적피질、해마신경원삼여료미주신경자격적항간작용.내장전입신식가능통과재뇌적피질、해마부위적정합작용체도유효지조억구체간성활동.
BACKGROUND: Vagus nerve stimulation (VNS) is a neurophysiological therapy (NPT) of refractory epilepsy, which can control the seizure by stimulating the vagus nerve stem in cervical part.OBJECTIVE: To study the effect of intermittent left-side VNS on seizure of epileptic animals, and provide theoretic basis for the interaction of somatic information and that of internal organs.DESIGN: Observation study.SETTING: Department of Neurobiology, Capital University of Medical Science.MATERIALS: The experiment was performed in the Laboratory of Electrophysiology in Department of Neurobiology, Capital University of Medical Science from March 2000 to September 2002. Thirty-four healthy adult SD rats and 8 rabbits, weighting (220-250) g and (2.2-2.5) kg respectively were selected.METHODS: ①Ten rats were intramuscularly injected with (150 000-160 000) U of penicillin (PCN). VNS effects on epileptiform activities of rats were studied by observing the changes in electrocorticogram (ECoG)and behavior of rats before and after VNS.②(0.24-0.48) mg of PCN was injected into the hippocampus of another 8 rabbits to induce epilepsy, and VNS effects on ECoG of epileptic rats were observed. ③Seizures of 16 rats were induced by Kainic acid, and changes in discharge activity of hippocampal neuron, ECoG and behavior of epileptic rats were observed after VNS. ④Seizures of 8 rabbits were induced by cortical injection of strychnine with microinjector, and VNS effects on ECoG of rabbits with epilepsy induced by acute cortical injury were observed.MAIN OUTCOME MEASURES: ①VNS effects on seizure of rats with epilepsy induced by PCN. ②VNS effects on seizure of rats with epilepsy induced by Kainic acid. ③VNS effects on epileptiform ECoG of rabbits with epilepsy induced by strychnine.RESULTS: A total of 34 rats and 8 rabbits were involved in the analysis of results. VNS could remarkably suppress the seizure of epileptic animals,and epileptiform ECoG, epileptiform discharges of hippocampal neuron and behavior significantly changed with the total effective rate greater than 50%. The total effective rate of VNS before seizure was greater than 80%.In epilepsy group indoeed by intramuscular injection of PCN, ECoG and behavior were markedlly aneliorated respectively for 40% and 50% of rats.In epilepsy group induced by injection of PCN in hippocampus, the ECoG was siguificantly ameliorated in 50% rats. In epileptic rabbit group induced by partial injection of strychnine via cerebral cortex, the epilepti form wave iu ECoG was controlled by VNS in 50 % of animals.CONCLUSION: VNS can effectively suppress seizure of epileptic animals. The antiepileptic effect of VNS is associated with cerebral cortical aud hippcampal neurons. Somatic epileptiform activity could be effectively inhibited by the integration of visceral afferent information in cortical and hippocampal parts.
