CAJ | 학술논문

建立了用神经递质多巴胺作电活性配体示差脉冲伏安法间接测定生物体液中的铝的方法.多巴胺在玻碳电极上的氧化峰电流随铝浓度的增加而线性下降.在最佳测定条件(pH 7.0,2.4×10-4 mol/L多巴胺,0.08 mol/L NH4Ac缓冲溶液)下,铝的检测下限、相对标准偏差(4×10-6 mol/L Al(Ⅲ),n=8)、线性范围分别为1.7×10-7mol/L、3.4%、4.0×10-7～42×10-7mol/L Al(Ⅲ).对许多生物体液中可能存在的物质进行了干扰试验.采用标准加入法将所建立的方法应用于实际样品如人全血、脑病患者的脑脊液、糖尿病患者的尿、肺癌患者的腹水、合成肾透析液及合成脑脊液等样品中铝测定,结果表明回收率在95%～108%之间,所测得的样品中铝含量与文献值和ICP法值吻合.通过紫外光谱、拉曼光谱及13C核磁共振谱探讨了测定方法的原理.加入铝后多巴胺氧化峰电流的下降原因是铝与多巴胺的邻羟基配位,从而使电活性的多巴胺浓度下降.
건립료용신경체질다파알작전활성배체시차맥충복안법간접측정생물체액중적려적방법.다파알재파탄전겁상적양화봉전류수려농도적증가이선성하강.재최가측정조건(pH 7.0,2.4×10-4 mol/L다파알,0.08 mol/L NH4Ac완충용액)하,려적검측하한、상대표준편차(4×10-6 mol/L Al(Ⅲ),n=8)、선성범위분별위1.7×10-7mol/L、3.4%、4.0×10-7～42×10-7mol/L Al(Ⅲ).대허다생물체액중가능존재적물질진행료간우시험.채용표준가입법장소건립적방법응용우실제양품여인전혈、뇌병환자적뇌척액、당뇨병환자적뇨、폐암환자적복수、합성신투석액급합성뇌척액등양품중려측정,결과표명회수솔재95%～108%지간,소측득적양품중려함량여문헌치화ICP법치문합.통과자외광보、랍만광보급13C핵자공진보탐토료측정방법적원리.가입려후다파알양화봉전류적하강원인시려여다파알적린간기배위,종이사전활성적다파알농도하강.
Neurotransmitter dopamine (3, 4-dihydroxyphenylethylamine, DA) has been adopted as an electroactive sequestering agent of aluminum (Al) for indirect determination of Al in biological fluids. It was observed that the decrease of the differential pulse voltammetric (DPV) anodic peak current of DA was linear with the increase of Al in the pH range from 6.0 to 7.6. Under optimum experimental conditions (pH 7.0, 2.4 × 10-4 mol/L DA, and 0.08 mol/L NH4Ac buffer solution),the detection limit of Al in the linear range from 4.0 × 10-7 to 42 × 10-7 mol/L is 1.7 × 10-7 mol/L and the relative standard deviation for 4.0 × 10-6 mol/L AlⅢ is 3.4% ( n = 8). Many foreign species possibly existing in biological fluids have been tested for interference. Recoveries between 95 %～108 % have been achieved in the application of the method to measurements of Al in biological samples such as synthetic renal dialysate, Ringer's solution, human blood, cerebrospinal fluid of patients, and urine of diabetic patients. The methodological principle that Al is complexed with DA on the electroactive site resulting in the depression of electroactive DA has been verified from the electrochemical evidences and the responses of UV-vis, Raman, and 13C NMR of DA both in the presence and absence of Al. This principle is applicable to both the chelants with the same electroactive functional groups as DA and similar cases such as monitoring electrochemical inactive species by electroactive ligands through complexation reactions.