CAJ | 학술논문

为了研究分析新疆罗布泊周边地区pH值5～6的盐湖嗜盐古菌资源.从湖中分离筛选出一批嗜盐古菌,对其进行了生理生化特性研究,发现其中6株菌的生理特性和产酶特性比较特殊,并采用PCR方法扩增出其16SrRNA基因(16S rDNA),并测定了基因的核苷酸序列.基于16S rDNA序列的同源性比较以及16S rDNA序列的系统发育学研究表明,菌株B20-RDX是盐盒菌属Haloarchaeon属中新种成员,GenBank登录号为FJ561285,该菌株为革兰氏阴性菌,最适盐浓度25%,最适pH 8.0,能产过氧化氢酶、淀粉酶,对四环素有抗性,能利用精氨酸和丁二酸盐.迄今为止,国内极少有关罗布泊周边地区极端环境微生物研究的报道,该研究可为今后研究同类极端环境中新的物种资源开发应用以及微生物多样性研究提供素材和参考.
위료연구분석신강라포박주변지구pH치5～6적염호기염고균자원.종호중분리사선출일비기염고균,대기진행료생리생화특성연구,발현기중6주균적생리특성화산매특성비교특수,병채용PCR방법확증출기16SrRNA기인(16S rDNA),병측정료기인적핵감산서렬.기우16S rDNA서렬적동원성비교이급16S rDNA서렬적계통발육학연구표명,균주B20-RDX시염합균속Haloarchaeon속중신충성원,GenBank등록호위FJ561285,해균주위혁란씨음성균,최괄염농도25%,최괄pH 8.0,능산과양화경매、정분매,대사배소유항성,능이용정안산화정이산염.흘금위지,국내겁소유관라포박주변지구겁단배경미생물연구적보도,해연구가위금후연구동류겁단배경중신적물충자원개발응용이급미생물다양성연구제공소재화삼고.
Objective:Study and analyze the species resource from a Salt Lake pH5-6 in the LopNur surrounding region,Xinjiang.A group of halophilic archaea strains isolated from a Salt Lake pH5-6 in the LopNur surrounding region,Xinjiang.And their physiological and biochemical characteristics were determined.Based on its morphology and physiological and biochemical features,16S rRNA encoding gene(16S rDNA) from six strains were amplified byPCR,and the nucleotide sequences were determined.The results indicated that the strain B20-RDX was was a novol species of the genus Haloarchaeon,The GenBank accession numbers of the 16S rDNA sequences about strain is FJ561285.The strain B20-RDX is Gram-negative balls,The optimum salt concentration for its growth is 25%,and the optimum pH is at 8.0.The strain B20-RDX produce catalases and amylases,which resistant to tetracycline.B20-RDX make use of arginine and succinate salts.Now,there are few report about Extreme halophiles from Lop Nur surrounding region,in China.The study has offered enlarge the boundary of halophilic archaea diversity and provide halophilic archaea resources for further theoretical and applied researches.