中国给水排水
中國給水排水
중국급수배수
CHINA WATER & WASTEWATER
2009年
23期
104-107
,共4页
丁西明%傅以钢%夏四清%赵建夫%库兹涅佐夫
丁西明%傅以鋼%夏四清%趙建伕%庫玆涅佐伕
정서명%부이강%하사청%조건부%고자열좌부
溶藻细菌%PCR-DGGE指纹分析技术%微生物群落结构%聚类分析
溶藻細菌%PCR-DGGE指紋分析技術%微生物群落結構%聚類分析
용조세균%PCR-DGGE지문분석기술%미생물군락결구%취류분석
algae-lysing bacteria%PCR-DGGE fingerprinting%microbial community structure%cluster analysis
从藻类消退期的河流中取水样,分别加入斜生栅藻、铜绿微囊藻、小球藻、人工湖泊中的两种混合野生藻及氧化塘中的野生藻,平行培养1周,考察水样中的溶藻细菌对这些藻类的去除效果.结果表明,对上述水样中的叶绿素a的去除率分别为94.69%、95.41%、94.50%、89.19%、92.67%和95.63%.采用PCR-DGGE指纹分析技术初步研究了加入不同藻类水样的微生物群落结构,结果表明加入藻类的水样与原始水样的群落结构存在较大差异,但加入不同藻类的水样之间的相似度较高.溶藻系统中的微生物种类包括未培养的细菌类、Bacillus sp.、Bacillus cere-us、Stenotrophomonas sp.等.
從藻類消退期的河流中取水樣,分彆加入斜生柵藻、銅綠微囊藻、小毬藻、人工湖泊中的兩種混閤野生藻及氧化塘中的野生藻,平行培養1週,攷察水樣中的溶藻細菌對這些藻類的去除效果.結果錶明,對上述水樣中的葉綠素a的去除率分彆為94.69%、95.41%、94.50%、89.19%、92.67%和95.63%.採用PCR-DGGE指紋分析技術初步研究瞭加入不同藻類水樣的微生物群落結構,結果錶明加入藻類的水樣與原始水樣的群落結構存在較大差異,但加入不同藻類的水樣之間的相似度較高.溶藻繫統中的微生物種類包括未培養的細菌類、Bacillus sp.、Bacillus cere-us、Stenotrophomonas sp.等.
종조류소퇴기적하류중취수양,분별가입사생책조、동록미낭조、소구조、인공호박중적량충혼합야생조급양화당중적야생조,평행배양1주,고찰수양중적용조세균대저사조류적거제효과.결과표명,대상술수양중적협록소a적거제솔분별위94.69%、95.41%、94.50%、89.19%、92.67%화95.63%.채용PCR-DGGE지문분석기술초보연구료가입불동조류수양적미생물군락결구,결과표명가입조류적수양여원시수양적군락결구존재교대차이,단가입불동조류적수양지간적상사도교고.용조계통중적미생물충류포괄미배양적세균류、Bacillus sp.、Bacillus cere-us、Stenotrophomonas sp.등.
The water samples were taken from the river in the decline period of algae. Scenedes-mus, Microcystis aeruginosa, Chlorella, two mixed wild algae from artificial lake and wild algae from eco-pond were mixed respectively with the water samples. The removal efficiencies of those algae by the al-gae-lysing bacteria in the water samples were investigated by parallel culture for a week. The results show that the removal rates of chlorophyll-a are 94. 69%, 95.41%, 94.50%, 89. 19%, 92.67% and 95.63% respectively. The microbial community structure in the water samples with different algae was preliminarily studied by using PCR-DGGE fingerprinting. The results show that there is a significant difference in the microbial community structure between the algae-added water and the original water. The microbial community structure among the water samples with different algae has higher similarity. The microbes in the algae-lysing system include the uncultured bacteria, Bacillus sp., Bacillus cereus and Stenotrophomonas sp.