中山大学学报（医学科学版）
中山大學學報（醫學科學版）
중산대학학보（의학과학판）
JOURNAL OF SUN YAT-SEN UNIVERSITY(MEDICAL SCIENCES)
2010年
1期
59-63
,共5页
黄俊%汪建平%王磊%刘焕亮%魏宜胜%黄美近%陈典克%傅新晖%陈静
黃俊%汪建平%王磊%劉煥亮%魏宜勝%黃美近%陳典剋%傅新暉%陳靜
황준%왕건평%왕뢰%류환량%위의성%황미근%진전극%부신휘%진정
结直肠癌%Survivin基因%单核苷酸多态性
結直腸癌%Survivin基因%單覈苷痠多態性
결직장암%Survivin기인%단핵감산다태성
colorectal cancer%survivin gene%single nucleotide polymorphism
[目的]探讨生存素Survivin基因启动子区-31C/G单核苷酸多态性与中国华南地区散发性结直肠癌(CRC)遗传易感性的关系. [方法]采用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)检测华南地区711例健康人和702例CRC的Survivin基因-31C/G位点单核苷酸多态性.[结果] 结直肠癌患者CC基因型的频率明显高于对照人群(36.35%vs.26.2%,X~2=17.89,P<0.001),与CC基因型相比,CG、GG基因型和等位基因G携带者的CRC发病风险分别显著下降至0.61倍(95%CI=0.46～0.80,P<0.001)、0.52倍(95%CI=0.38～0.71,P<0.001)和0.58倍(95%CI=0.45～0.74,P<0.001).[结论] Survivin基因-31C/G多态性与CRC发病风险有关,-31G变异基因型是中国南方人群散发性结直肠癌独立保护因素.
[目的]探討生存素Survivin基因啟動子區-31C/G單覈苷痠多態性與中國華南地區散髮性結直腸癌(CRC)遺傳易感性的關繫. [方法]採用聚閤酶鏈反應-限製性片段長度多態性法(PCR-RFLP)檢測華南地區711例健康人和702例CRC的Survivin基因-31C/G位點單覈苷痠多態性.[結果] 結直腸癌患者CC基因型的頻率明顯高于對照人群(36.35%vs.26.2%,X~2=17.89,P<0.001),與CC基因型相比,CG、GG基因型和等位基因G攜帶者的CRC髮病風險分彆顯著下降至0.61倍(95%CI=0.46～0.80,P<0.001)、0.52倍(95%CI=0.38～0.71,P<0.001)和0.58倍(95%CI=0.45～0.74,P<0.001).[結論] Survivin基因-31C/G多態性與CRC髮病風險有關,-31G變異基因型是中國南方人群散髮性結直腸癌獨立保護因素.
[목적]탐토생존소Survivin기인계동자구-31C/G단핵감산다태성여중국화남지구산발성결직장암(CRC)유전역감성적관계. [방법]채용취합매련반응-한제성편단장도다태성법(PCR-RFLP)검측화남지구711례건강인화702례CRC적Survivin기인-31C/G위점단핵감산다태성.[결과] 결직장암환자CC기인형적빈솔명현고우대조인군(36.35%vs.26.2%,X~2=17.89,P<0.001),여CC기인형상비,CG、GG기인형화등위기인G휴대자적CRC발병풍험분별현저하강지0.61배(95%CI=0.46～0.80,P<0.001)、0.52배(95%CI=0.38～0.71,P<0.001)화0.58배(95%CI=0.45～0.74,P<0.001).[결론] Survivin기인-31C/G다태성여CRC발병풍험유관,-31G변이기인형시중국남방인군산발성결직장암독립보호인소.
[Objective] To investigate the association between -31C/G polymorphism in the promoter of survivin gene and the susceptibility to sporadic colorectal cancer in southern Chinese population. [Methods] Survivin-31C/G genotypes were determined by polymerase chain reaction-restriction fragment length polymorpbism (PCR-RFLP) in 711 healthy controls and 702 CRC cases. [Results] The number of CRC patients carrying with CC genotype was much higher than those of controls (36.5 % vs. 26.12%, X~2=17.89, P<0.001). Compared with CC genotypes, CG, GG genotypes and G allele carriers had a significantly decreased risk of CRC, with the decrease being 0.61-fold (95% CI=0.46-0.81, P<0.001), 0.52-fold (95% CI=0.38-0.71, P<0.001) and 0.58-fold (95% CI=0.45-0.74, P<0.001), respectively. [Conclusion] Survivin gene -31C/G polymorphism is associated with sporadic CRC risk, the G variant genotypo is the independent protective factors against sporadic CRC in soutbem Chinese population.
