CAJ | 학술논문

目的观察过氧化物酶Ⅰ (PrxⅠ)在正常和骨关节炎(OA)膝关节软骨组织中的表达和分布特点,探讨PrxⅠ与活性氧歧化物和凋亡等在OA软骨表层聚集现象之间的关联.方法分别从正常膝关节提取正常软骨( NC,n=21)和接受膝关节表面置换术的OA患者提取OA软骨(OA,n=21),应用Western blot技术检测PrxⅠ在正常软骨细胞和OA软骨细胞中表达水平的总体差异,并用免疫组织化学技术观察PrxⅠ蛋白在正常与OA软骨表/中/深各层组织表达和分布的特点.结果 Western blot证实PrxⅠ在OA软骨组织中的表达水平较正常软骨组织显著提高2.89倍(t=18.34,P＜0.01).免疫组织化学显示Prx Ⅰ在正常软骨组织的表层、中层和深层呈现较均一的表达,但是,在OA软骨组织中,Prx Ⅰ的表达水平存在显著层间差异.Prx Ⅰ在软骨组织深层表达水平显著升高,但在浅层细胞中,PrxⅠ的表达水平反而显著减低甚至缺如.结论虽然总体表达水平升高,但PrxⅠ在OA软骨组织表层的表达缺如,可能与OA软骨组织表层活性氧歧化物蓄积和细胞凋亡聚集现象相关.
목적 관찰과양화물매Ⅰ (PrxⅠ)재정상화골관절염(OA)슬관절연골조직중적표체화분포특점,탐토PrxⅠ여활성양기화물화조망등재OA연골표층취집현상지간적관련.방법 분별종정상슬관절제취정상연골( NC,n=21)화접수슬관절표면치환술적OA환자제취OA연골(OA,n=21),응용Western blot기술검측PrxⅠ재정상연골세포화OA연골세포중표체수평적총체차이,병용면역조직화학기술관찰PrxⅠ단백재정상여OA연골표/중/심각층조직표체화분포적특점.결과 Western blot증실PrxⅠ재OA연골조직중적표체수평교정상연골조직현저제고2.89배(t=18.34,P＜0.01).면역조직화학현시Prx Ⅰ재정상연골조직적표층、중층화심층정현교균일적표체,단시,재OA연골조직중,Prx Ⅰ적표체수평존재현저층간차이.Prx Ⅰ재연골조직심층표체수평현저승고,단재천층세포중,PrxⅠ적표체수평반이현저감저심지결여.결론 수연총체표체수평승고,단PrxⅠ재OA연골조직표층적표체결여,가능여OA연골조직표층활성양기화물축적화세포조망취집현상상관.
Objective To investigate the differential expression and distribution of peroxiredoxin Ⅰ (Prx Ⅰ) in normal and osteoarthritic knee cartilage,thus to understand the possible correlation between Prx Ⅰ and the superficial accumulating phenomena of reactive oxygen species ( ROS ) and chondrocyte apoptosis in osteoarthritis (OA) cartilage.Methods Normal cartilage ( NC ) was obtained from the normal donors ( n =21 ) and osteoarthritic cartilage was harvested from patients who had undergone total knee arthroplasty (n =21 ).The overall expression difference of Prx Ⅰ was compared using Western blot;zonal expression of Prx Ⅰ inside the normal and osteoarthritic cartilage was detected by immunohistochemistry.Results Overall expression of Prx Ⅰ in osteoarthritic chondrocytes was upregulated 2.89 fold compared with that of normal cartilage (t =18.34,P ＜0.01 ).Immumohistochemistry showed that the expression of Prx Ⅰ was uniformly in the superficial,middle and deep layes of normal cartilage.But in OA cartilage,the expression of Prx Ⅰ showed significant differences in the three layers.Prx Ⅰ expression was up-regulated dominantly in chondrocytes of the deep layer,while the expression was slight,even absent in the superficial layer.Conclusions Although the overall expression of Prx Ⅰ was up regulated,its expression was down- regulated dramatically in the superficial layer,which might be aetiologically correlated with the accumulation of ROS and chondrocyte apoptosis in the superficial layer of osteoarthritic cartilage.