国际遗传学杂志
國際遺傳學雜誌
국제유전학잡지
INTERNATIONAL JOURNAL OF GENETICS
2011年
4期
202-212
,共11页
龚莎莎%郑静%张婷%郑斌娇%方芳%吕建新%管敏鑫
龔莎莎%鄭靜%張婷%鄭斌嬌%方芳%呂建新%管敏鑫
공사사%정정%장정%정빈교%방방%려건신%관민흠
氨基糖甙类抗生素%耳毒性%耳聋%线粒体DNA突变%修饰因子
氨基糖甙類抗生素%耳毒性%耳聾%線粒體DNA突變%脩飾因子
안기당대류항생소%이독성%이롱%선립체DNA돌변%수식인자
Aminoglycosides%Ototoxicity%Hearing loss%Mitochondrial DNA mutations%Modifier factors
线粒体12S rRNA基因是引起氨基糖甙类抗生素耳毒性和非综合征型耳聋的突变热点区域。其中,位于高度保守的12S rRNA基因解码区的同质性1555A>G和1494C>T突变,可导致部分患者对氨基糖甙类抗生素超敏感。当发生1555A>G或1494C>T突变时,12S rRNA高度保守的A位就会形成新的1494C-G1555或1494U-A1555碱基配对,使得人类线粒体核糖体的结构与细菌核糖体更加相似,以致氨基糖甙类抗生素与12S rRNA的结合更加容易,从而解释了为何携带这些突变的个体在使用了氨基糖甙类抗生素后会出现或加重耳聋表型。相关功能研究表明,无论是否存在氨基糖甙类抗生素的作用,携带1555A>G或1494C>T突变的细胞均会出现线粒体蛋白合成缺陷,并随之引发细胞呼吸功能障碍。此外,携带这些突变的家系,其母系成员听力损失程度、发病年龄和外显率均存在较大差异,提示核修饰基因、线粒体单体型以及氨基糖甙类抗生素等对12S rRNA 1555A>G和1494C>T突变的表型表达起着修饰作用。这些研究成果为以下三个方面提供了科学依据:①预测个体耳毒性风险;②提高氨基糖甙类抗生素治疗的安全性;③降低耳聋发生率。
線粒體12S rRNA基因是引起氨基糖甙類抗生素耳毒性和非綜閤徵型耳聾的突變熱點區域。其中,位于高度保守的12S rRNA基因解碼區的同質性1555A>G和1494C>T突變,可導緻部分患者對氨基糖甙類抗生素超敏感。噹髮生1555A>G或1494C>T突變時,12S rRNA高度保守的A位就會形成新的1494C-G1555或1494U-A1555堿基配對,使得人類線粒體覈糖體的結構與細菌覈糖體更加相似,以緻氨基糖甙類抗生素與12S rRNA的結閤更加容易,從而解釋瞭為何攜帶這些突變的箇體在使用瞭氨基糖甙類抗生素後會齣現或加重耳聾錶型。相關功能研究錶明,無論是否存在氨基糖甙類抗生素的作用,攜帶1555A>G或1494C>T突變的細胞均會齣現線粒體蛋白閤成缺陷,併隨之引髮細胞呼吸功能障礙。此外,攜帶這些突變的傢繫,其母繫成員聽力損失程度、髮病年齡和外顯率均存在較大差異,提示覈脩飾基因、線粒體單體型以及氨基糖甙類抗生素等對12S rRNA 1555A>G和1494C>T突變的錶型錶達起著脩飾作用。這些研究成果為以下三箇方麵提供瞭科學依據:①預測箇體耳毒性風險;②提高氨基糖甙類抗生素治療的安全性;③降低耳聾髮生率。
선립체12S rRNA기인시인기안기당대류항생소이독성화비종합정형이롱적돌변열점구역。기중,위우고도보수적12S rRNA기인해마구적동질성1555A>G화1494C>T돌변,가도치부분환자대안기당대류항생소초민감。당발생1555A>G혹1494C>T돌변시,12S rRNA고도보수적A위취회형성신적1494C-G1555혹1494U-A1555감기배대,사득인류선립체핵당체적결구여세균핵당체경가상사,이치안기당대류항생소여12S rRNA적결합경가용역,종이해석료위하휴대저사돌변적개체재사용료안기당대류항생소후회출현혹가중이롱표형。상관공능연구표명,무론시부존재안기당대류항생소적작용,휴대1555A>G혹1494C>T돌변적세포균회출현선립체단백합성결함,병수지인발세포호흡공능장애。차외,휴대저사돌변적가계,기모계성원은력손실정도、발병년령화외현솔균존재교대차이,제시핵수식기인、선립체단체형이급안기당대류항생소등대12S rRNA 1555A>G화1494C>T돌변적표형표체기착수식작용。저사연구성과위이하삼개방면제공료과학의거:①예측개체이독성풍험;②제고안기당대류항생소치료적안전성;③강저이롱발생솔。
The mitochondrial 12 S rRNA is a hot spot for mutations associated with both aminoglycoside-induced and nonsyndromic hearing loss. Of those, the homoplasmic 1555A > G and 1494C > T mutations at a highly conserved decoding region of the 12 S rRNA have been associated with hearing loss.These two mutations account for a significant number of cases of aminoglycoside ototoxicity. The 15 5 5 A >G or 1494C > T mutation is expected to form novel 1494C-G1555 or 1494U-A1555 base-pair at the highly conserved A-site of 12S rRNA. These transitions make the human mitochondrial ribosome more bacteria-like, alter binding sites for aminoglycosides, thereby accounting for the fact that the exposure to aminoglycosides can induce or worsen hearing loss in individuals carrying one of these mutations.Biochemical characterization demonstrated an impairment of mitochondrial protein synthesis and subsequent defects in respiration in cells carrying the C1494T or A1555G mutation, in the presence or absence of aminoglycosides. Furthermore, a wide range of severity, age-at-onset and penetrance of hearing loss was observed within and among families carrying these mutations. Nuclear modifier genes, mitochondrial haplotypes and aminoglycosides should modulate the phenotypic manifestation of the 12 S rRNA 1555 A > G and 1494C > T mutations. Therefore, these data provide valuable information and technology to predict: ①which individuals are at risk for ototoxicity ; ② to improve the safety of aminoglycoside antibiotic therapy ;③ eventually to decrease the incidence of deafness..