CAJ | 학술논문

目的研究不同浓度的葡萄糖对体外培养条件下的健康人外周血内皮祖细胞(endothelial progenitor cells,EPCs)凋亡的影响.方法经密度梯度离心法分离出健康成年人外周血中的单个核细胞(mononuclear cells,MNCs),在体外诱导分化后,用FITC标记的荆豆凝集素Ⅰ(Dil-acLDL)和Dil标记的乙酰化低密度脂蛋白(FITC-UEA-1)双染色鉴定为内皮祖细胞.观察不同浓度的葡萄糖对所获取的内皮祖细胞凋亡的影响.结果 11.1 mmol/L葡萄糖干预组较5.6 mmol/L葡萄糖干预组的凋亡率差异无统计学意义(P＞0.05);25.5 mmol/L葡萄糖干预组的凋亡率较5.6 mmol/L葡萄糖干预组的差异有统计学意义(P＜0.05),且随干预时间的延长凋亡率增加(P＜0.05).结论高浓度葡萄糖能增加体外培养条件下人外周血内皮祖细胞的凋亡,且这种作用呈时间依赖性.
목적 연구불동농도적포도당대체외배양조건하적건강인외주혈내피조세포(endothelial progenitor cells,EPCs)조망적영향.방법 경밀도제도리심법분리출건강성년인외주혈중적단개핵세포(mononuclear cells,MNCs),재체외유도분화후,용FITC표기적형두응집소Ⅰ(Dil-acLDL)화Dil표기적을선화저밀도지단백(FITC-UEA-1)쌍염색감정위내피조세포.관찰불동농도적포도당대소획취적내피조세포조망적영향.결과 11.1 mmol/L포도당간예조교5.6 mmol/L포도당간예조적조망솔차이무통계학의의(P＞0.05);25.5 mmol/L포도당간예조적조망솔교5.6 mmol/L포도당간예조적차이유통계학의의(P＜0.05),차수간예시간적연장조망솔증가(P＜0.05).결론 고농도포도당능증가체외배양조건하인외주혈내피조세포적조망,차저충작용정시간의뢰성.
Objectives To investigate the effects of glucose on apoptosis rate of cultured endothelial progenitor cells (cEPCs). Methods The peripheral blood of healthy adults was isolated by density gradient centrifugation, and mononuclear cells (MNCs) were inducted to differentiate at cultured conditions.EPCs were identified by Dil-acLDL and FITC-UEA-1 as double fluorescent-positive cells. The effectsof glucose at different concentrations on apoptosis rate of the harvested EPCs were measured by fluorescent microscope and fluorescence-activated cell sorting (FACS) after staining with Annexin V-FITC and PI. Results No significant differences were observed in apoptosis rate between samples treatedwith 5.6mmol/l glucose and 11. 1 mmol/l. P ＞0. 05). 25.5 mmol/L glucose enhanced the EPCsapoptosis rate in a time-dependent manner( P ＜0. 05). Conclusion High concentration glucose can accelerate apoptosis rate of EPCs in a time-dependent manner.