CAJ | 학술논문

目的探讨钙超负荷与高血压肾小动脉重建的关系及氯沙坦(Losartan)的治疗作用.方法正常血压Wistar-Kyoto(WKY)大鼠和同种的16周龄自发高血压大鼠(spontaneously hypertensive rat,SHR)大鼠随机分为WKY对照组、SHR对照组、高剂量losartan组(SHR+HL)、低剂量losartan组(SHR+LL).每组6只动物.给予正常饮食,饲养至24周.losartan溶于10 ml生理盐水中灌胃,余组等量生理盐水灌胃.测量尾动脉血压、肾组织钙离子浓度、肾小动脉内羟脯氨酸含量并观察肾脏病理测量肾入球小动脉中膜厚度、血管内径及中膜厚度与内径比值(MT/LR).结果 SHR组肾组织钙离子浓度[(18.42±2.34)μmol/g]、肾小动脉内羟脯氨酸含量[(8.26±2.02)mg/g]均大于WKY组[(11.83±1.98)μmol/g,(5.16±0.98)mg/g](t＝3.116,3.258,P＜0.05),但两治疗组均有所降低,小于SHR组(t＝2.946,P＜0.05).SHR组肾内小动脉的壁厚[(5.25±1.13)μm]、壁厚内径比[(9.57±1.78)%]均大于WKY组相应比值[(4.03±0.16)μm,(7.12±1.35)%](t＝2.836,3.425,P＜0.05),但两治疗组壁厚内径比[(7.64±1.29)%,(7.85±1.32)%]小于SHR组(t＝3.512,3.648,P＜0.05).结论 losartan可以通过抑制细胞内钙超负荷、降低纤维化程度来改善肾小动脉的血管阻力,对高血压大鼠的肾小动脉重建有逆转作用.
목적 탐토개초부하여고혈압신소동맥중건적관계급록사탄(Losartan)적치료작용.방법 정상혈압Wistar-Kyoto(WKY)대서화동충적16주령자발고혈압대서(spontaneously hypertensive rat,SHR)대서수궤분위WKY대조조、SHR대조조、고제량losartan조(SHR+HL)、저제량losartan조(SHR+LL).매조6지동물.급여정상음식,사양지24주.losartan용우10 ml생리염수중관위,여조등량생리염수관위.측량미동맥혈압、신조직개리자농도、신소동맥내간포안산함량병관찰신장병리측량신입구소동맥중막후도、혈관내경급중막후도여내경비치(MT/LR).결과 SHR조신조직개리자농도[(18.42±2.34)μmol/g]、신소동맥내간포안산함량[(8.26±2.02)mg/g]균대우WKY조[(11.83±1.98)μmol/g,(5.16±0.98)mg/g](t＝3.116,3.258,P＜0.05),단량치료조균유소강저,소우SHR조(t＝2.946,P＜0.05).SHR조신내소동맥적벽후[(5.25±1.13)μm]、벽후내경비[(9.57±1.78)%]균대우WKY조상응비치[(4.03±0.16)μm,(7.12±1.35)%](t＝2.836,3.425,P＜0.05),단량치료조벽후내경비[(7.64±1.29)%,(7.85±1.32)%]소우SHR조(t＝3.512,3.648,P＜0.05).결론 losartan가이통과억제세포내개초부하、강저섬유화정도래개선신소동맥적혈관조력,대고혈압대서적신소동맥중건유역전작용.
Objective To investigate the effects of losartan on aortic elasticity and remodeling in spontaneously hypertensive rats (spontaneously hypertensive rats SHR). Methods WKY (Wistar - Kyoto ) rats with normal blood pressure and 16 weeks spontaneously hypertensive rats were randomly divided into WKY control group, SHR control group, high dose losartan group (SHR + HL), low doses losartan group (SHR + LL). Each group has six animals which were given normal diet for 24 weeks. Losartan which was dissolved in 10 ml physiological saline was filling in stomach, other groups were filling with physiological saline. Tail arterial blood pressure, kidney tissues calcium concentration, renal small artery hydroxyproline content was measured and small arteries wall thickness of Glomerularwas detected, and the ratio of thickness and inner diameter (MT/LR) in kidney pathological were observed. Results The calcium concentration of SHR group in kidney tissues was [(18.42±2.34)μmol/g], kidney small artery hydroxyproline content was [(8.26±2.02)mg/g], which were greater than WKY group [(11.83±1.98)μmol/g,(5.16±0.98)mg/g] (t＝3.116,3.258,P＜0.05), but the two treatment groups were less than SHR group (t＝2.946,P＜0.05), the difference was significant. Small arteries wall thickness of Glomerular was [(5.25±1.13)μm], the ratio of thickness and inner diameter (MT/LR) was [(9.57±1.78)%], which were greater than WKY group[(4.03±0.16)μm ,(7.12±1.35)%](t＝2.836,3.425,P＜0.05), but the wall thickness of two treatment groups were [(7.64±1.29)%,(7.85±1.32)%], the two treatment groups were less than SHR group (t＝3.512,3.648,P＜0.05). Conclusions Losartanmay inhibit intracellular calcium overload, reduce fibrosis degree and improve renal arteriole resistance and reverse the renal arteriole reconstruction of SHR rats.