中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2012年
5期
313-316
,共4页
张晶%赵晨燕%刘清华%余党会%陈颖%史敏%倪灿荣%朱明华
張晶%趙晨燕%劉清華%餘黨會%陳穎%史敏%倪燦榮%硃明華
장정%조신연%류청화%여당회%진영%사민%예찬영%주명화
胰腺肿瘤%微RNAs%微阵列分析%基因表达谱
胰腺腫瘤%微RNAs%微陣列分析%基因錶達譜
이선종류%미RNAs%미진렬분석%기인표체보
Pancreatic neoplasms%MicroRNA%Microarray analysis%Gene expression profiling
目的 应用microRNA( miRNA)高通量生物芯片筛选胰腺导管腺癌及癌旁组织差异表达的miRNA,分析其相关的靶基因.方法 收集9例新鲜的胰腺导管腺癌和3例癌旁组织,运用标记713个miRNA的Agilent miRNA生物芯片筛选胰腺导管腺癌差异表达的miRNA,应用荧光实时定量PCR方法验证表达上调的miRNA.采用TargetScan 5.1和miRandaV5分析软件分析差异表达miRNAs的靶基因.结果 miRNA芯片筛选出11个胰腺导管腺癌相关的差异表达的miRNA,其中miR-194*、miR-192*、miR-602、miR-194表达上调,miR-139-3p、miR-513a-5p、miR-630、miR-30c-1*、miR-887、miR-508-5p、miR-516a-5p表达下调.miR-192、miR-194及其同源体的表达在31例胰腺癌组织中得到验证.经软件分析,miR.192靶基因有ZEB2、CXCL-2、EEF1A1、ERCC3,miR-192*靶基因有DCC、SMAD4、FAS,miR-194靶基因有DACH1、IGSF11、PTPN2、RBBP4,miR-194*靶基因有CD40LG、CIDEB、FHL1.结论胰腺导管腺癌存在11个表达差异的miRNA,这些miRNA可能与胰腺导管腺癌的发生、发展有关.
目的 應用microRNA( miRNA)高通量生物芯片篩選胰腺導管腺癌及癌徬組織差異錶達的miRNA,分析其相關的靶基因.方法 收集9例新鮮的胰腺導管腺癌和3例癌徬組織,運用標記713箇miRNA的Agilent miRNA生物芯片篩選胰腺導管腺癌差異錶達的miRNA,應用熒光實時定量PCR方法驗證錶達上調的miRNA.採用TargetScan 5.1和miRandaV5分析軟件分析差異錶達miRNAs的靶基因.結果 miRNA芯片篩選齣11箇胰腺導管腺癌相關的差異錶達的miRNA,其中miR-194*、miR-192*、miR-602、miR-194錶達上調,miR-139-3p、miR-513a-5p、miR-630、miR-30c-1*、miR-887、miR-508-5p、miR-516a-5p錶達下調.miR-192、miR-194及其同源體的錶達在31例胰腺癌組織中得到驗證.經軟件分析,miR.192靶基因有ZEB2、CXCL-2、EEF1A1、ERCC3,miR-192*靶基因有DCC、SMAD4、FAS,miR-194靶基因有DACH1、IGSF11、PTPN2、RBBP4,miR-194*靶基因有CD40LG、CIDEB、FHL1.結論胰腺導管腺癌存在11箇錶達差異的miRNA,這些miRNA可能與胰腺導管腺癌的髮生、髮展有關.
목적 응용microRNA( miRNA)고통량생물심편사선이선도관선암급암방조직차이표체적miRNA,분석기상관적파기인.방법 수집9례신선적이선도관선암화3례암방조직,운용표기713개miRNA적Agilent miRNA생물심편사선이선도관선암차이표체적miRNA,응용형광실시정량PCR방법험증표체상조적miRNA.채용TargetScan 5.1화miRandaV5분석연건분석차이표체miRNAs적파기인.결과 miRNA심편사선출11개이선도관선암상관적차이표체적miRNA,기중miR-194*、miR-192*、miR-602、miR-194표체상조,miR-139-3p、miR-513a-5p、miR-630、miR-30c-1*、miR-887、miR-508-5p、miR-516a-5p표체하조.miR-192、miR-194급기동원체적표체재31례이선암조직중득도험증.경연건분석,miR.192파기인유ZEB2、CXCL-2、EEF1A1、ERCC3,miR-192*파기인유DCC、SMAD4、FAS,miR-194파기인유DACH1、IGSF11、PTPN2、RBBP4,miR-194*파기인유CD40LG、CIDEB、FHL1.결론이선도관선암존재11개표체차이적miRNA,저사miRNA가능여이선도관선암적발생、발전유관.
Objective To study the differentially expressed microRNA (miRNA) between pancreatic ductal adenocarcinoma (PDAC) and para-cancerous tissues,and determine related target genes.Methods Nine fresh PDAC tumor tissues and 3 adjacent normal pancreatic tissues were collected,then Agilent miRNAmicroarray with 713 miRNA loci was used to identify the differentially expressed miRNA.Real-time PCR method was applied to verify the up-regulated miRNA.TargetScan 5.1 and miRandaV5 software were used to analyze the related target genes.Results miRNA microarray identified 11 PDAC related miRNAs,among them,the expressions of miR-194*,miR-192*,miR-602,miR-194 were up-regulated,while the expressions of miR-139-3p,miR-513a-5p,miR-630,miR-30c-1 *,miR-887,miR-508-5p,miR-516a-5p were down-regulated.The expressions of miR-192,miR-194 and their homolog were verified in 31 PDAC tumor tissues.After software analysis,it was found that target genes of miR-192 were ZEB2,CXCL-2,EEF1A1,ERCC3,and target genes of miR-192 * were DCC,SMAD4,FAS,and target genes of miR-194 included DACHI,IGSF11,PTPN2,RBBP4,while target genes of miR-194 * included CD40LG,CIDEB,FHL1.Conclusions Eleven differentially expressed miRNAs are present in PDC,and they may be involved in the occurrence and development of PDC.
