CAJ | 학술논문

目的构建细胞程序死亡因子1配体1(PD-L1)的重组表达质粒,在原核系统中表达并分析其生物学活性.方法经密码子优化后合成PD-L1全基因序列,构建硫氧还原蛋白-pET43b/(PD-L1)重组表达质粒并在大肠埃希菌中表达;用ELISA验证表达产物与其受体结合的生物学活性.结果正确构建了PD-L1重组表达质粒及其大肠埃菌基因工程菌,能稳定、高效地表达目的蛋白,相对分子质量为47×103,目的蛋白能与其受体特异性结合.结论成功获得有生物学活性的PD-L1重组蛋白,为研制单克隆抗体及其与病毒感染慢性化的关系提供基础条件.
목적 구건세포정서사망인자1배체1(PD-L1)적중조표체질립,재원핵계통중표체병분석기생물학활성.방법 경밀마자우화후합성PD-L1전기인서렬,구건류양환원단백-pET43b/(PD-L1)중조표체질립병재대장애희균중표체;용ELISA험증표체산물여기수체결합적생물학활성.결과 정학구건료PD-L1중조표체질립급기대장애균기인공정균,능은정、고효지표체목적 단백,상대분자질량위47×103,목적 단백능여기수체특이성결합.결론 성공획득유생물학활성적PD-L1중조단백,위연제단극륭항체급기여병독감염만성화적관계제공기출조건.
Objective To construct the programmed cell death 1 ligant 1 (PD-L1)recombination expression vector,express the fusion protein in prokaryotic and analyze the biological action of express product. Methods The whole PD-L1 gene sequence was synthesized after codon optimized. Construct the thioredoxin-(PD-L1) recombination expression vector and express the fusion protein in E. coli. Purified the target protein and analyze the conjugated ability of protein by ELISA. Results The PD-L1 recombinant expression vector has been constructed correctly. The target protein has been obtained with which expressed in high efficiency and production. The target protein can conjugate specifically with the PD-1, its specific receptor. Conclusion We have obtained the PD-L1 recombinant protein success with high biological activity. The result provide the basic condition for further study on antibody and mutually action between PD-L1 and chronic virus infectious.