中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2010年
8期
726-730
,共5页
徐同江%叶飞%田春雷%谢蕊繁%舒凯%郭东生%雷霆
徐同江%葉飛%田春雷%謝蕊繁%舒凱%郭東生%雷霆
서동강%협비%전춘뢰%사예번%서개%곽동생%뢰정
Gsp癌基因%GHSR-1a%Ghrelin
Gsp癌基因%GHSR-1a%Ghrelin
Gsp암기인%GHSR-1a%Ghrelin
Gsp oncogene%GHSR - 1a%Ghrelin
目的 研究垂体生长激素(GH)腺瘤组织中Ghrelin、生长激素释放剂受体-1a亚型(GHSR-1a)mRNA的表达,并探讨其表达与gsp癌基因的关系.方法 采用PCR-DNA直接序列分析方法,观察43例垂体GH腺瘤组织中gsp癌基因的表达;应用实时荧光定量PCR检测Ghrelin和GHSR-1a mRNA的表达水平;统计学分析Ghrelin和GHSR-1a mRNA表达与gsp癌基因的关系.结果 Ghrelin mRNA表达在gsp癌基因阳性、阴性组织间差异无统计学意义(P＞0.05);gsp阳性组织中GHSR-1a mRNA表达明显高于gsp阴性组织(P＜0.05);gsp阳性、阴性腺瘤组织中GHSR-1amRNA与Ghrelin mRNA的表达均呈明显正相关(R=0.592或0.544,P＜0.05).结论 垂体GH腺瘤中gsp癌基因上调GHSR-1a mRNA的表达;而对Ghrelin mRNA表达无明显影响.gsp阳性、阴性腺瘤中Ghrelin均可正向调控GHSR-1a mRNA表达.
目的 研究垂體生長激素(GH)腺瘤組織中Ghrelin、生長激素釋放劑受體-1a亞型(GHSR-1a)mRNA的錶達,併探討其錶達與gsp癌基因的關繫.方法 採用PCR-DNA直接序列分析方法,觀察43例垂體GH腺瘤組織中gsp癌基因的錶達;應用實時熒光定量PCR檢測Ghrelin和GHSR-1a mRNA的錶達水平;統計學分析Ghrelin和GHSR-1a mRNA錶達與gsp癌基因的關繫.結果 Ghrelin mRNA錶達在gsp癌基因暘性、陰性組織間差異無統計學意義(P＞0.05);gsp暘性組織中GHSR-1a mRNA錶達明顯高于gsp陰性組織(P＜0.05);gsp暘性、陰性腺瘤組織中GHSR-1amRNA與Ghrelin mRNA的錶達均呈明顯正相關(R=0.592或0.544,P＜0.05).結論 垂體GH腺瘤中gsp癌基因上調GHSR-1a mRNA的錶達;而對Ghrelin mRNA錶達無明顯影響.gsp暘性、陰性腺瘤中Ghrelin均可正嚮調控GHSR-1a mRNA錶達.
목적 연구수체생장격소(GH)선류조직중Ghrelin、생장격소석방제수체-1a아형(GHSR-1a)mRNA적표체,병탐토기표체여gsp암기인적관계.방법 채용PCR-DNA직접서렬분석방법,관찰43례수체GH선류조직중gsp암기인적표체;응용실시형광정량PCR검측Ghrelin화GHSR-1a mRNA적표체수평;통계학분석Ghrelin화GHSR-1a mRNA표체여gsp암기인적관계.결과 Ghrelin mRNA표체재gsp암기인양성、음성조직간차이무통계학의의(P＞0.05);gsp양성조직중GHSR-1a mRNA표체명현고우gsp음성조직(P＜0.05);gsp양성、음성선류조직중GHSR-1amRNA여Ghrelin mRNA적표체균정명현정상관(R=0.592혹0.544,P＜0.05).결론 수체GH선류중gsp암기인상조GHSR-1a mRNA적표체;이대Ghrelin mRNA표체무명현영향.gsp양성、음성선류중Ghrelin균가정향조공GHSR-1a mRNA표체.
Objective To investigate the relationship between the expression of growth hormone secretagogue receptor type 1a (GHSR- 1a) 、Ghrelin mRNA and gsp oncogene in human GH- secreting pituitary adenomas. Methods The gsp oncogene mutation in 43 cases of GH -secreting pituitary adenomas was detected by PCR - DNA direct sequencing analysis. The expression of GHSR - 1a、Ghrelin mRNA was determined by SYBR green real -time fluorescent quantitative PCR. Then the relationships between the expression of GHSR -1a、Ghrelin mRNA and gsp oncegene were studied with statistical methods. Results There was no significant difference in the expression of ghrelin mRNA between mutation - positive and mutation - negative adenomas. The expression of GHSR - 1a mRNA was significantly higher in gsp - positive adenomas than that in gsp - negative adenomas. There was significant direct correlation between the levels of ghrelin mRNA and GHSR mRNA expression in gsp - positive and gsp - negative adenomas. Conclusions Gsp oncogene may up - regulate the expression of GHSR - 1a mRNA and has no effect on the expression of ghrelin mRNA in human GH - secreting pituitary adenomas. In addition, ghrelin could elevate the expression of GHSR - 1a mRNA in GH - secreting pituitary adenomas with or without gsp mutations.
