中华神经科杂志
中華神經科雜誌
중화신경과잡지
Chinese Journal of Neurology
2008年
11期
731-733
,共3页
脑缺血%脑血管意外%高半胱氨酸%胱硫醚β合酶%多态现象,遗传
腦缺血%腦血管意外%高半胱氨痠%胱硫醚β閤酶%多態現象,遺傳
뇌결혈%뇌혈관의외%고반광안산%광류미β합매%다태현상,유전
Brain ischemia%Cerebrovaseular accident%Homocysteine%Cystathionine beto-synthase%Polymorphism,genetic
目的 探讨血浆同型半胱氨酸(Hcy)水平、胱硫醚β合酶(CBS)基因T833C多态与缺血性脑血管病(ICVD)的相关性.方法 应用高效液相色谱仪-FD法和扩增阻滞突变体系法(ARMS)检测360例ICVD患者和210名健康体检者的血浆Hcy水平和CBS T833C基因型,并进行相关分析.结果 ICVD组和对照组空腹血浆Hcy浓度分别为(17.6±4.8)μmol/L和(13.3±4.3)μmol/L,差异有统计学意义(t=10.716,P<0.05).ICVD组CBS基因第833位点T/T、T/C、C/C和基因型频率与对照组比较差异无统计学意义(χ2=o.785,P>0.05),C等位基因频率与对照组差异也无统计学意义(χ2=0.941,P>0.05).ICVD组各亚组CBS基因第833位点T/T和T/C+C/C基因型频率比较差异无统计学意义(χ2=1.691,P>0.05),C等位基因频率比较差异也无统计学意义(χ2=1.617,P>0.05).CBS T833C不同基因型之间血浆Hcy水平不同(F=6.56,P<0.05),TC、CC基因型组血浆Hcy水平明显高于TT基因型组,TC、CC基因型组间血浆Hcy水平差异无统计学意义.结论 血浆Hcy水平升高可能为ICVD的危险因素,CBS T833C基因多态可能不是ICVD的独立危险因子.
目的 探討血漿同型半胱氨痠(Hcy)水平、胱硫醚β閤酶(CBS)基因T833C多態與缺血性腦血管病(ICVD)的相關性.方法 應用高效液相色譜儀-FD法和擴增阻滯突變體繫法(ARMS)檢測360例ICVD患者和210名健康體檢者的血漿Hcy水平和CBS T833C基因型,併進行相關分析.結果 ICVD組和對照組空腹血漿Hcy濃度分彆為(17.6±4.8)μmol/L和(13.3±4.3)μmol/L,差異有統計學意義(t=10.716,P<0.05).ICVD組CBS基因第833位點T/T、T/C、C/C和基因型頻率與對照組比較差異無統計學意義(χ2=o.785,P>0.05),C等位基因頻率與對照組差異也無統計學意義(χ2=0.941,P>0.05).ICVD組各亞組CBS基因第833位點T/T和T/C+C/C基因型頻率比較差異無統計學意義(χ2=1.691,P>0.05),C等位基因頻率比較差異也無統計學意義(χ2=1.617,P>0.05).CBS T833C不同基因型之間血漿Hcy水平不同(F=6.56,P<0.05),TC、CC基因型組血漿Hcy水平明顯高于TT基因型組,TC、CC基因型組間血漿Hcy水平差異無統計學意義.結論 血漿Hcy水平升高可能為ICVD的危險因素,CBS T833C基因多態可能不是ICVD的獨立危險因子.
목적 탐토혈장동형반광안산(Hcy)수평、광류미β합매(CBS)기인T833C다태여결혈성뇌혈관병(ICVD)적상관성.방법 응용고효액상색보의-FD법화확증조체돌변체계법(ARMS)검측360례ICVD환자화210명건강체검자적혈장Hcy수평화CBS T833C기인형,병진행상관분석.결과 ICVD조화대조조공복혈장Hcy농도분별위(17.6±4.8)μmol/L화(13.3±4.3)μmol/L,차이유통계학의의(t=10.716,P<0.05).ICVD조CBS기인제833위점T/T、T/C、C/C화기인형빈솔여대조조비교차이무통계학의의(χ2=o.785,P>0.05),C등위기인빈솔여대조조차이야무통계학의의(χ2=0.941,P>0.05).ICVD조각아조CBS기인제833위점T/T화T/C+C/C기인형빈솔비교차이무통계학의의(χ2=1.691,P>0.05),C등위기인빈솔비교차이야무통계학의의(χ2=1.617,P>0.05).CBS T833C불동기인형지간혈장Hcy수평불동(F=6.56,P<0.05),TC、CC기인형조혈장Hcy수평명현고우TT기인형조,TC、CC기인형조간혈장Hcy수평차이무통계학의의.결론 혈장Hcy수평승고가능위ICVD적위험인소,CBS T833C기인다태가능불시ICVD적독립위험인자.
Objective To investigate the correlation between cystathionine β-synthase (CBS) T833C gene polymorphism and the plasma homocysteine (Hey) levels in patients with ischemic cerebral vascular disease(ICVD). Methods Three hundred and sixty patients with ICVD and 210 control subjects were enrolled. The T833C polymorphism of CBS gene was analyzed by the amplification refractory mutation system (ARMS). Plasma Hey levels were measured by high performance liquid chromatography-fluorescence detection(HPLC-FD). Results Plasma Hey levels in the ICVD group ((17.6±4.8) μmol/L) were higher than those in control group ((13.3±4.3) μmol/L, t = 10.716, P< 0.05) . There were no differences in genotype frequencies and allele frequencies between the ICVD group and contral group (χ2 = 0.785, 0.941 ,P>0.05). Plasma Hey levels in CC genotype or TC genotype were higher than those in TT genotype (F = 6.56, P< 0.05). Condusions High plasma Hey level is an independent risk factor of ICVD,but CBS T833C polymorphism may not associated with ICVD.
