CAJ | 학술논문

目的通过活体动物实验,探究低频振动对骨髓基质干细胞(BMSC)骨向分化调控能力及其OPG、RANKL基因表达的影响.方法 BMSC结合骨基质明胶(BMG)移植联合低频振动刺激治疗骨缺损,随机分为非振动对照组和不同频率振动组,振动组于第7天开始接受振动干预5周,振动结束后对不同频率振动组别BMSC OPG、RANKL mRNA进行检测.结果振动组BMSC OPG、RANKL基因表达明显上调(P＜0.05),以25 Hz显著(P＜0.01);100 Hz振动组BMSC OPG、RANKL基因表达下调(P＜0.05).结论低频振动可调控BMSC的骨向分化,可能通过调节OPG/RANKLmRNA的表达直接促进骨形成,抑制骨吸收.
목적 통과활체동물실험,탐구저빈진동대골수기질간세포(BMSC)골향분화조공능력급기OPG、RANKL기인표체적영향.방법 BMSC결합골기질명효(BMG)이식연합저빈진동자격치료골결손,수궤분위비진동대조조화불동빈솔진동조,진동조우제7천개시접수진동간예5주,진동결속후대불동빈솔진동조별BMSC OPG、RANKL mRNA진행검측.결과 진동조BMSC OPG、RANKL기인표체명현상조(P＜0.05),이25 Hz현저(P＜0.01);100 Hz진동조BMSC OPG、RANKL기인표체하조(P＜0.05).결론 저빈진동가조공BMSC적골향분화,가능통과조절OPG/RANKLmRNA적표체직접촉진골형성,억제골흡수.
Objective To explore the effect of low frequency vibration (LFV)on the osteogenic differentiation regulating capability of bone marrow stromal cell (BMSC) and the expressions of OPG (osteoprotegerin) mRNA and RANKL (nuclear factor kappa B ligand) mRNA through living animal experiment. Methods Both BMSC transplantation and low-frequency vibration were employed to treat bone defects. The groups were randomized into non-vibration and vibration of different frequencies. The vibration group received vibrating interventions at Day 7 for 5 weeks. After vibrations, the BMSC OPG and RANKL mRNA of different frequency groups were detected. Results The BMSC OPG and RANKL gene expressions significantly increased ( P ＜ 0. 05 ), especially at 25 Hz (P ＜ 0. 01 ). And for the vibration group at 100 Hz,the BMSC OPG and RANKL gene expressions decreased ( P ＜ 0. 05). Conclusion Low-frequency vibration may promote the osteogenic differentiation capability of BMSC probably through regulating the OPG/RANKL mRNA expression, directly promoting bone formation and inhibiting bone resorption.