CAJ | 학술논문

鹅掌楸(Liriodendron chinense(Hemsl.) Sarg.)是中国特有珍稀树种.鉴于目前还鲜有野生鹅掌楸组培过程生理生化变化的相关报道.本研究以峨嵋山野生鹅掌楸茎尖芽为外植体进行组织培养,并对其继代培养再生过程中超氧阴离子自由基(O_2~-)、过氧化氢(H_2O_2)和丙二醛(MDA)含量的变化进行了测定.研究结果表明,随着培养时间的延长,植物体中O_2~-和MDA含量呈现逐渐增加的变化趋势.这表明在连续继代培养条件下,植物体内活性氧会积累,引起膜脂过氧化.H_2O_2含量"S"形曲线变化,并随着再生芽的分化而上升,因此,H_2O_2不仅参与了膜脂过氧化过程,还可能作为一种细胞信号物质参与诱导细胞分化和再生芽的形成.该研究也为进一步用组织培养法保存峨眉山珍稀植物鹅掌楸种质资源提供了参考.
아장추(Liriodendron chinense(Hemsl.) Sarg.)시중국특유진희수충.감우목전환선유야생아장추조배과정생리생화변화적상관보도.본연구이아미산야생아장추경첨아위외식체진행조직배양,병대기계대배양재생과정중초양음리자자유기(O_2~-)、과양화경(H_2O_2)화병이철(MDA)함량적변화진행료측정.연구결과표명,수착배양시간적연장,식물체중O_2~-화MDA함량정현축점증가적변화추세.저표명재련속계대배양조건하,식물체내활성양회적루,인기막지과양화.H_2O_2함량"S"형곡선변화,병수착재생아적분화이상승,인차,H_2O_2불부삼여료막지과양화과정,환가능작위일충세포신호물질삼여유도세포분화화재생아적형성.해연구야위진일보용조직배양법보존아미산진희식물아장추충질자원제공료삼고.
Chinese tulip tree (Liriodendron chinense (Hemsl.) Sarg) is an endemic rare tree species in China.Whereas the physiological and biochemical changes of it during the tissue culture was quite few reported yet.In this research,we employed shoot-tips of wild Chinese tulip tree in Mount Emei as explants for tissue culture,and mes ured the content changes of super oxygen-anion flee radicals (O_2~-),hydrogen peroxide (H_2O_2) and malondialdehyde (MDA) during the secondary culture.The results demonstrated that the content of O_2~- and MDA increased gradually along with the continuation of the culture time,which illustrated that the active oxygen accumulated in continuous secondary culture,causing membrane lipid peroxidation.Furthermore,the content of H_2O_2 changed in S-shaped curve,and increased with the differentiation of the regenerated bud.As a result,we inferred that H_2O_2 might be not only involved in lipid peroxidation,but also in cell differentiation induction and regenerated bud formation as a signal molecule.This research would also facilitate the germplasm conservation of rare plant Chinese tulip tree in Mount Emei by employing tissue culture approach.