CAJ | 학술논문

目的探讨影响冻融胚胎复苏率和移植成功率的相关因素.方法回顾性分析573个冷冻胚胎移植(FET)周期的临床资料,按冷冻前胚胎质量、胚胎发育时间、解冻后胚胎复苏程度和冷冻方法分别进行分组,分析不同分类各组间的胚胎复苏情况和(或)临床妊娠率差异.结果冷冻当天胚胎中有优质胚胎者的临床妊娠率显著高于无优质胚胎者(31.8% vs 20.0%)(P<0.05).受精第2天冷冻者与受精第3天冷冻者的胚胎复苏率和临床妊娠率差异无统计学意义(79.1% vs 82.9%和25.5% vs31.2%)(P>0.05).解冻后完全移植100%卵裂球完整者和混合移植者的临床妊娠率显著高于完全移植部分卵裂球损伤者(36.7% vs 24.1%和29.2% vs 24.1%)(P<0.05).冷冻方法改进后的胚胎复苏率和全胚复苏率显著高于冷冻方法改进前(82.0% vs 66.3%和50.0% vs 27.5%)(P<0.05).结论胚胎质量与胚胎复苏率和临床妊娠率密切相关,冷冻胚胎解冻后全胚复苏率与临床妊娠率正相关,冷冻过程中的操作细节是胚胎实验室保证冻融胚胎复苏、移植成功的重要因素.
목적 탐토영향동융배태복소솔화이식성공솔적상관인소.방법 회고성분석573개냉동배태이식(FET)주기적림상자료,안냉동전배태질량、배태발육시간、해동후배태복소정도화냉동방법분별진행분조,분석불동분류각조간적배태복소정황화(혹)림상임신솔차이.결과 냉동당천배태중유우질배태자적림상임신솔현저고우무우질배태자(31.8% vs 20.0%)(P<0.05).수정제2천냉동자여수정제3천냉동자적배태복소솔화림상임신솔차이무통계학의의(79.1% vs 82.9%화25.5% vs31.2%)(P>0.05).해동후완전이식100%란렬구완정자화혼합이식자적림상임신솔현저고우완전이식부분란렬구손상자(36.7% vs 24.1%화29.2% vs 24.1%)(P<0.05).냉동방법개진후적배태복소솔화전배복소솔현저고우냉동방법개진전(82.0% vs 66.3%화50.0% vs 27.5%)(P<0.05).결론 배태질량여배태복소솔화림상임신솔밀절상관,냉동배태해동후전배복소솔여림상임신솔정상관,냉동과정중적조작세절시배태실험실보증동융배태복소、이식성공적중요인소.
Objective To explore the impact factors for post-thaw embryo survival rate and clinical pregnancy rate in frozen-thawed embryo transfer program. Methods The clinical data of 573 cycles of frozen-thawed embryo transfers were retrospectively analysed. Groups were divided according to the pre-freeze embryo quality, pre-freeze embryonic developmental stage, frozen-thawed embryo quality and cryopreservation technique, respectively, and post-thaw embryo survival rates and/or clinical pregnancy rates were compared among groups. Results The clinical pregnancy rate of high quality pre-freeze embryo was significantly higher than that of low quality pre-freeze embryo (31.8% vs 20.0%) (P< 0.05). There was no significant difference in the post-thaw survival rates and clinical pregnancy rates between embryos frozen at day 2 of ferrtilization and those frozen at day 3 of ferrtilization(79. 1% vs 82.9% and 25.5% vs 31.2%, respectively) (P>0.05). The clinical pregnancy rates of the transfer cycles only with fully intact embryos and with mixed embryos were significantly higher than that only with partially damaged embryos(36.7% vs 24.1% and 29.2% vs 24.1%, respectively)(P<0.05). The post-thaw survival rate and post-thaw high-quality embryo rate were significantly higher in those processed with modified cryopreservation technique than in those processed with original cryopreservation technique (82.0% vs 66.3% and 50.0% vs 27.5%, respectively)(P<0.05). Conclusion Pre-freeze embryo quality, post-thaw embryo survival rate and post-thaw embryo quality have a positive correlation to subsequent clinical pregnancy rate. Favorable cryopreservation technique may ensure the success of post-thaw embryo recovery and transfer.