解剖学报
解剖學報
해부학보
ACTA ANATOMICA SINICA
2009年
6期
881-885
,共5页
王海涛%刘昊%徐爱军%阚泉%高俊玲
王海濤%劉昊%徐愛軍%闞泉%高俊玲
왕해도%류호%서애군%감천%고준령
慢性强迫游泳应激%海马%钙离子%钙调蛋白依赖性激酶Ⅱ%免疫电镜%免疫印迹%反转录-聚合酶链式反应%大鼠
慢性彊迫遊泳應激%海馬%鈣離子%鈣調蛋白依賴性激酶Ⅱ%免疫電鏡%免疫印跡%反轉錄-聚閤酶鏈式反應%大鼠
만성강박유영응격%해마%개리자%개조단백의뢰성격매Ⅱ%면역전경%면역인적%반전록-취합매련식반응%대서
Chronic forced swimming stress%Hippocampus%Calcium%Calmodulin dependent protein kinase Ⅱ%Immunoelectron microscopy%Westen blotting%RT-PCR%Rat
目的 探讨慢性强迫游泳应激(CFSS)模型大鼠行为学的改变和海马神经元Ca~(2+)/钙调蛋白依赖性激酶Ⅱ(CaMKⅡ)的表达变化. 方法 成年健康雄性Wistar大鼠60只,随机分为对照组(30只)和慢性强迫游泳应激组(30只).慢性强迫游泳组强迫游泳4周,制备慢性强迫游泳应激模型;糖水偏好实验、开场实验和Morris水迷宫检测大鼠行为学改变;荧光探针标记法测定海马神经元内Ca~(2+)浓度;胶体金免疫电镜、免疫印迹和RT-PCR检测CaMKⅡ的表达变化. 结果 慢性强迫游泳应激组糖水消耗量和糖水偏好百分比分别为4.114±0.644和86.610±4.450,对照组为8.157±1.105和94.930±2.893,差异有统计学意义(P<0.01);开场实验中慢性强迫游泳应激组和对照组的直立次数分别为1.75±0.96和6.00±0.82,差异有统计学意义(P<0.05);水迷宫实验逃避潜伏期分别为(20.762±3.236)s和(5.632±1.065)s,差异有统计学意义(P<0.01);海马神经元内游离Ca~(2+)浓度分别为(498.94±40.45)nmol/L和(288.91±32.42)nmol/L,差异有统计学意义(P<0.01);CaMKⅡ蛋白和mRNA相对表达水平均高于对照组(P<0.01). 结论 海马Ca~(2+)及CaMKⅡ的表达上调,可能是抑郁模型大鼠情感行为异常的病理生理基础之一.
目的 探討慢性彊迫遊泳應激(CFSS)模型大鼠行為學的改變和海馬神經元Ca~(2+)/鈣調蛋白依賴性激酶Ⅱ(CaMKⅡ)的錶達變化. 方法 成年健康雄性Wistar大鼠60隻,隨機分為對照組(30隻)和慢性彊迫遊泳應激組(30隻).慢性彊迫遊泳組彊迫遊泳4週,製備慢性彊迫遊泳應激模型;糖水偏好實驗、開場實驗和Morris水迷宮檢測大鼠行為學改變;熒光探針標記法測定海馬神經元內Ca~(2+)濃度;膠體金免疫電鏡、免疫印跡和RT-PCR檢測CaMKⅡ的錶達變化. 結果 慢性彊迫遊泳應激組糖水消耗量和糖水偏好百分比分彆為4.114±0.644和86.610±4.450,對照組為8.157±1.105和94.930±2.893,差異有統計學意義(P<0.01);開場實驗中慢性彊迫遊泳應激組和對照組的直立次數分彆為1.75±0.96和6.00±0.82,差異有統計學意義(P<0.05);水迷宮實驗逃避潛伏期分彆為(20.762±3.236)s和(5.632±1.065)s,差異有統計學意義(P<0.01);海馬神經元內遊離Ca~(2+)濃度分彆為(498.94±40.45)nmol/L和(288.91±32.42)nmol/L,差異有統計學意義(P<0.01);CaMKⅡ蛋白和mRNA相對錶達水平均高于對照組(P<0.01). 結論 海馬Ca~(2+)及CaMKⅡ的錶達上調,可能是抑鬱模型大鼠情感行為異常的病理生理基礎之一.
목적 탐토만성강박유영응격(CFSS)모형대서행위학적개변화해마신경원Ca~(2+)/개조단백의뢰성격매Ⅱ(CaMKⅡ)적표체변화. 방법 성년건강웅성Wistar대서60지,수궤분위대조조(30지)화만성강박유영응격조(30지).만성강박유영조강박유영4주,제비만성강박유영응격모형;당수편호실험、개장실험화Morris수미궁검측대서행위학개변;형광탐침표기법측정해마신경원내Ca~(2+)농도;효체금면역전경、면역인적화RT-PCR검측CaMKⅡ적표체변화. 결과 만성강박유영응격조당수소모량화당수편호백분비분별위4.114±0.644화86.610±4.450,대조조위8.157±1.105화94.930±2.893,차이유통계학의의(P<0.01);개장실험중만성강박유영응격조화대조조적직립차수분별위1.75±0.96화6.00±0.82,차이유통계학의의(P<0.05);수미궁실험도피잠복기분별위(20.762±3.236)s화(5.632±1.065)s,차이유통계학의의(P<0.01);해마신경원내유리Ca~(2+)농도분별위(498.94±40.45)nmol/L화(288.91±32.42)nmol/L,차이유통계학의의(P<0.01);CaMKⅡ단백화mRNA상대표체수평균고우대조조(P<0.01). 결론 해마Ca~(2+)급CaMKⅡ적표체상조,가능시억욱모형대서정감행위이상적병리생리기출지일.
Objective To observe the changes of behavior, intracellular free calcium and the expression of calmodulin dependent protein kinase Ⅱ(CaMKⅡ) in the hippocampal neurons of chronic forced swimming stress rats. Methods Male Wistar rats were randomly divided into control group and chronic forced swimming stress group. The behavior was examined using sucrose preference test, open-filed test and Morris water maze. The intracellular free calcium was examined by fluorescence spectrophotometer. The expression of CaMKⅡ was detected using colloidal gold immunoelectron microscopy technique, Western blotting and RT-PCR. Results The consumption of sucrose and erect quantity of chronic forced swimming stress group were lower than those of control group(P<0.01, P<0.05). The escape latency time in Morries water maze test of chronic forced swimming stress group was higher than that of control group(P<0.01). The intracellular free calcium level and the expression of CaMKⅡ in the hippocampus was higher than that of control group(P<0.01).Conclusion The lasting dysfunction of Ca~(2+)/CaMKⅡ signaling cascades in hippocampus may play important roles in the pathogenesis of chronic forced swimming stress rats.
