CAJ | 학술논문

首次用谷胱甘肽(GSH)作为稳定剂,在水溶液中制备了稳定地发射绿色荧光和橙色荧光的两种CdSe/CdS核/壳结构的纳米量子点.用紫外-可见分光光度和荧光光谱方法研究了CdSe/CdS量子点的发光特性.透射电镜(TEM)结果表明Cdse/CdS最子点近似球形,在水中分散性良好,比CdSe量子点具有更优异的发光特性,发射光谱和吸收光谱都有红移现象.将CdSe/Gds量子点与鼠抗人CD3抗体连接,制备了水溶件Cdse/CdS-CD3复合物探针,对人血淋巴细胞进行标记和成像.结果表明用该探针对人血淋巴细胞成像清晰,其荧光在30 min的连续蓝光激发下无明显衰退,而FITC荧光在20 min内基本完全猝灭.
수차용곡광감태(GSH)작위은정제,재수용액중제비료은정지발사록색형광화등색형광적량충CdSe/CdS핵/각결구적납미양자점.용자외-가견분광광도화형광광보방법연구료CdSe/CdS양자점적발광특성.투사전경(TEM)결과표명Cdse/CdS최자점근사구형,재수중분산성량호,비CdSe양자점구유경우이적발광특성,발사광보화흡수광보도유홍이현상.장CdSe/Gds양자점여서항인CD3항체련접,제비료수용건Cdse/CdS-CD3복합물탐침,대인혈림파세포진행표기화성상.결과표명용해탐침대인혈림파세포성상청석,기형광재30 min적련속람광격발하무명현쇠퇴,이FITC형광재20 min내기본완전졸멸.
Two kinds of L-glutathione capped highly fluorescent CdSe/CdS core-shell quantum dots (QDs) emitting green and orange fluorescence at 350 nm excitation were firstly prepared by an aqueous approach and used as fluorescent labels, to link mouse anti-human CD3 which was expressed on human T-lymphocyte. UV-Vis absorption and fluorescence emission spectra of the as-prepared CdSe/CdS core-shell QDs were studied. Compared with the CdSe QDs, a remarkable enhancement in the emission intensity and a red shift of emission wavelength of CdSe/CdS core-shell QDs was observed for the two kinds of QDs emitting green and orange fluorescence. The TEM results showed that the as prepared CdSe and CdSe/CdS QDs dispersed well in aqueous solution, and their shape was approximately spherical, and the CdSe/CdS QDs nano particles emitting green fluorescence are of about 5 nm in diameter. The two kinds of CdSe/CdS QDs were linked with mouse anti-human CD_3 to image human T-lymphocyte. The fluorescent microscopical images of human T-lymphocyte labeled with CdSe/CdS QDs-CD3 and F1TC-CD3 demonstrated that the fluorescent CdSe/CdS QDs exhibited much better photo stability and brighter fluorescence than FITC, showing a good application potential in the immuno-labeling of cells.