东北农业大学学报
東北農業大學學報
동북농업대학학보
JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY
2010年
1期
86-92
,共7页
陈侠君%王炳谦%刘奕%范兆廷
陳俠君%王炳謙%劉奕%範兆廷
진협군%왕병겸%류혁%범조정
虹鳟%血细胞%显微结构
虹鱒%血細胞%顯微結構
홍준%혈세포%현미결구
rainbow trout: peripheral blood cells: microstructure
用光学显微镜对二倍体和三倍体虹鳟外周血细胞大小及显微结构,尤其是对三倍体特有的畸形红细胞进行了研究.结果表明,两种倍性虹鳟的红细胞及细胞核、嗜中性粒细胞、单核细胞体积均为3:2的比例关系;各类白细胞从多到少依次为血栓细胞、淋巴细胞、嗜中性粒细胞和单核细胞;两种倍性虹鳟红细胞形态存在明显差异,三倍体虹鳟红细胞短径,长径比值为0.57,近17%有哑铃形细胞核或双核;二倍体虹鳟红细胞短径/长径比值为0.67,且未见哑铃形和双核红细胞;在三倍体中观察到很多哑铃形的血栓细胞.
用光學顯微鏡對二倍體和三倍體虹鱒外週血細胞大小及顯微結構,尤其是對三倍體特有的畸形紅細胞進行瞭研究.結果錶明,兩種倍性虹鱒的紅細胞及細胞覈、嗜中性粒細胞、單覈細胞體積均為3:2的比例關繫;各類白細胞從多到少依次為血栓細胞、淋巴細胞、嗜中性粒細胞和單覈細胞;兩種倍性虹鱒紅細胞形態存在明顯差異,三倍體虹鱒紅細胞短徑,長徑比值為0.57,近17%有啞鈴形細胞覈或雙覈;二倍體虹鱒紅細胞短徑/長徑比值為0.67,且未見啞鈴形和雙覈紅細胞;在三倍體中觀察到很多啞鈴形的血栓細胞.
용광학현미경대이배체화삼배체홍준외주혈세포대소급현미결구,우기시대삼배체특유적기형홍세포진행료연구.결과표명,량충배성홍준적홍세포급세포핵、기중성립세포、단핵세포체적균위3:2적비례관계;각류백세포종다도소의차위혈전세포、림파세포、기중성립세포화단핵세포;량충배성홍준홍세포형태존재명현차이,삼배체홍준홍세포단경,장경비치위0.57,근17%유아령형세포핵혹쌍핵;이배체홍준홍세포단경/장경비치위0.67,차미견아령형화쌍핵홍세포;재삼배체중관찰도흔다아령형적혈전세포.
The size and microstructure of peripheral blood cells in diploid and triploid rainbow trout and especially its characteristic, al deformed rbcs were studied by the light microscopy. The results showed that the size of the erythrocyte and cell nucleus, neutrophil, monocyte in triploid and diploid fish had the propotion of 3:2; The number of the two kinds of rainbow trout leucocyte in decreasing order of thrombocyte, lymphocyte, neutrophil and monocyte; The erythrocyte of tdploid rainbow trout had the long ellipse with the width being 0.57 of the length and 17% of the erythrocyte had the dumbbell nucleus and two nuclei, whereas the erythrocyte of diploid rainbow trout tended to be round with the width being 0.67 of the length, but none of the erythrocyte with the dumbbell nucleus or two nuclei was found.