CAJ | 학술논문

通过组织分离法获得合欢枯萎病纯茵种，并以常规方法对合欢枯萎病病茵的生物学特性进行了研究，其结果表明，该茵可在PDA、PSA以及合欢的根、茎、叶培养基上生长，在PDA及PSA上的生长情况基本一致，且茵丝旺盛，而在合欢根、茎、叶培养基中的生长情况稍差；该茵的生长温度为5～32℃，最适生长温度为23—29℃；孢子在葡萄糖培养液中的发芽率最高，在根部培养液中的发芽情况较差；茵丝生长的pH值范围为3～12，但中性偏碱的环境条件更适合该茵生长。
통과조직분리법획득합환고위병순인충，병이상규방법대합환고위병병인적생물학특성진행료연구，기결과표명，해인가재PDA、PSA이급합환적근、경、협배양기상생장，재PDA급PSA상적생장정황기본일치，차인사왕성，이재합환근、경、협배양기중적생장정황초차；해인적생장온도위5～32℃，최괄생장온도위23—29℃；포자재포도당배양액중적발아솔최고，재근부배양액중적발아정황교차；인사생장적pH치범위위3～12，단중성편감적배경조건경괄합해인생장。
Fusarium oxysporum pernciosium was gotten through tissue separation and the biological characteristics was studied by common methods. The results showed that F. oxysporum could grow on PDA, PSA as well as on the medium （ADA） containing the roots. F. oxysporum have the similar growth on PDA and PSA, but weaker on ADA. The growth temperature of the F. oxysporum was 5 - 32℃, and the optimum temperature range was 23 - 29℃. The germination rate of the spore was the highest in the glucose solution and was lower in the root extract solution. The pH value for culturing the myceliun limits from 3 - 12 and neutral - alkali environment condition was more suitable for its growth.