国际皮肤性病学杂志
國際皮膚性病學雜誌
국제피부성병학잡지
INTERNATIONAL JOURNAL OF DERMATOLOGY AND VENEREOLOGY
2010年
6期
306-308
,共3页
刘春%齐淑贞%龙福泉%戴婷%王千秋
劉春%齊淑貞%龍福泉%戴婷%王韆鞦
류춘%제숙정%룡복천%대정%왕천추
梅毒%白细胞介素4%Toll样受体2%多态性,单核苷酸%疾病遗传易感性
梅毒%白細胞介素4%Toll樣受體2%多態性,單覈苷痠%疾病遺傳易感性
매독%백세포개소4%Toll양수체2%다태성,단핵감산%질병유전역감성
Syphilis%Interleukin-4%Toll-like receptor 2%Polymorphism,single nucleotide%Genetic predisposition to diseae
目的 探讨IL-4基因-590C/T位点、Toll样受体2(TLR2)基因Arg753Gln位点单核苷酸多态性与梅毒遗传易感性之间是否存在关联.方法 分别采用实时荧光定量PCR技术和DNA测序法检测56例早期显性梅毒患者、38例早期潜伏梅毒患者和102例正常人对照组IL-4基因-590C/T位点、TLR2基因Arg753Gln位点单核苷酸多态性.结果 早期梅毒组IL-4-590位点C、T等位基因的分布频率分别为0.229、0.771,对照组IL-4-590位点C、T等位基因的分布频率分别为0.216、0.784,两组相比差异无统计学意义(P＞0.05);显性梅毒组IL-4-590位点C、T等位基因的分布频率分别为0.232、0.768,潜伏梅毒组IL-4-590位点C、T等位基因的分布频率分别为0.224、0.776,两组相比差异无统计学意义(P＞0.05).各组TLR2Arg753Gln位点基因型均为GG型,GA、AA型缺如.早期梅毒组TLR2 Arg753Gln位点G、A等位基因的分布频率与对照组TLR2 Arg753Gln位点G、A等位基因的分布频率相比差异无统计学意义;显性梅毒组TLR2 Arg753Gln位点G、A等位基因的分布频率与潜伏梅毒组TLR2 Arg753Gln位点G、A等位基因的分布频率相比差异无统计学意义.结论 IL-4基因-590C/T位点、TLR2基因Arg753Gln位点单核苷酸多态性与梅毒遗传易感性无相关性.
目的 探討IL-4基因-590C/T位點、Toll樣受體2(TLR2)基因Arg753Gln位點單覈苷痠多態性與梅毒遺傳易感性之間是否存在關聯.方法 分彆採用實時熒光定量PCR技術和DNA測序法檢測56例早期顯性梅毒患者、38例早期潛伏梅毒患者和102例正常人對照組IL-4基因-590C/T位點、TLR2基因Arg753Gln位點單覈苷痠多態性.結果 早期梅毒組IL-4-590位點C、T等位基因的分佈頻率分彆為0.229、0.771,對照組IL-4-590位點C、T等位基因的分佈頻率分彆為0.216、0.784,兩組相比差異無統計學意義(P＞0.05);顯性梅毒組IL-4-590位點C、T等位基因的分佈頻率分彆為0.232、0.768,潛伏梅毒組IL-4-590位點C、T等位基因的分佈頻率分彆為0.224、0.776,兩組相比差異無統計學意義(P＞0.05).各組TLR2Arg753Gln位點基因型均為GG型,GA、AA型缺如.早期梅毒組TLR2 Arg753Gln位點G、A等位基因的分佈頻率與對照組TLR2 Arg753Gln位點G、A等位基因的分佈頻率相比差異無統計學意義;顯性梅毒組TLR2 Arg753Gln位點G、A等位基因的分佈頻率與潛伏梅毒組TLR2 Arg753Gln位點G、A等位基因的分佈頻率相比差異無統計學意義.結論 IL-4基因-590C/T位點、TLR2基因Arg753Gln位點單覈苷痠多態性與梅毒遺傳易感性無相關性.
목적 탐토IL-4기인-590C/T위점、Toll양수체2(TLR2)기인Arg753Gln위점단핵감산다태성여매독유전역감성지간시부존재관련.방법 분별채용실시형광정량PCR기술화DNA측서법검측56례조기현성매독환자、38례조기잠복매독환자화102례정상인대조조IL-4기인-590C/T위점、TLR2기인Arg753Gln위점단핵감산다태성.결과 조기매독조IL-4-590위점C、T등위기인적분포빈솔분별위0.229、0.771,대조조IL-4-590위점C、T등위기인적분포빈솔분별위0.216、0.784,량조상비차이무통계학의의(P＞0.05);현성매독조IL-4-590위점C、T등위기인적분포빈솔분별위0.232、0.768,잠복매독조IL-4-590위점C、T등위기인적분포빈솔분별위0.224、0.776,량조상비차이무통계학의의(P＞0.05).각조TLR2Arg753Gln위점기인형균위GG형,GA、AA형결여.조기매독조TLR2 Arg753Gln위점G、A등위기인적분포빈솔여대조조TLR2 Arg753Gln위점G、A등위기인적분포빈솔상비차이무통계학의의;현성매독조TLR2 Arg753Gln위점G、A등위기인적분포빈솔여잠복매독조TLR2 Arg753Gln위점G、A등위기인적분포빈솔상비차이무통계학의의.결론 IL-4기인-590C/T위점、TLR2기인Arg753Gln위점단핵감산다태성여매독유전역감성무상관성.
Objective To assess the relationship of IL-4 -590C/T polymorphism and TLR2 Arg753Gln polymorphism with genetic susceptibility to syphilis. Methods The study included 56 patients with early symptomatic syphilis, 38 patients with early latent syphilis and 102 normal human controls. Real-time quantitative fluorescent PCR was used to detect the IL-4 -590C/T polymorphism, and DNA sequencing to analyze the TLR2 Arg753Gln polymorphism, in these subjects. Results The frequency of C and T alleles at 590 position of IL-4 gene was respectively 0.229 and 0.771 in patients with early syphilis, 0.216 and 0.784 in the normal controls, 0.232 and 0.768 in patients with early symptomatic syphilis, 0.224 and 0.776 in patients with early latent syphilis. GG genotype was observed at position 753 in TLR2 gene in all the subjects. No significant difference was observed for the frequency of C or T alleles at position 590 in IL-4 gene or the frequency of A or G alleles at position 753 in TLR2 gene between patients with early syphilis and normal controls or between patients with early latent syphilis and those with early symptomatic syphilis (all P ＞ 0.05).Conclusion There seems no evident association between the susceptibility to syphilis and IL-4 -590C/T polymorphism or TLR2 Arg753Gln polymorphism.
