中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2009年
4期
273-276
,共4页
贫血%再生障碍性%细胞凋亡%抗原%CD34%抗原%CD95%Bcl-2原癌基因蛋白质
貧血%再生障礙性%細胞凋亡%抗原%CD34%抗原%CD95%Bcl-2原癌基因蛋白質
빈혈%재생장애성%세포조망%항원%CD34%항원%CD95%Bcl-2원암기인단백질
Anemia%aplastie%Apoptosis%Antigens%CD34%Antigens%CD95%Bcl-2 proto-oncogene proteins
目的 探讨再生障碍性贫血模型小鼠骨髓造血细胞凋亡过程中CD95和Bcl-2的表达以及相互之间的关系.方法 用辐射和免疫介导的方法 制造小鼠的再生障碍性贫血模型.用流式细胞术分析模型组和正常对照组CD34+细胞和CD45+细胞,以及细胞表面CD95和胞内Bcl-2的表达.结果 模型组骨髓细胞中低表达CD45的CD34+细胞(CD34+CD45low细胞)比例低于对照组[(0.85±0.19)%比(1.21±0.28)%,P<0.05].有核细胞中,CD95在模型组中的表达比例明显高于对照组[(66.30±7.68)%比(42.78±15.12)%],在这2组CD34+CD45+细胞的表达分别为(69.21±9.36)%和(22.31±3.33)%,CD34+CD45low细胞的表达分别为(53.70±16.46)%和(7.68±4.42)%,均P<0.01.Bcl-2的表达比例仅在模型组CD34+CD45low细胞中增加,与对照组相比差异有统计学意义[(7.60±2.40)%比(0.70±0.64)%,P<0.05].对照组细胞的CD95与Bcl-2表达无相关,而模型组CD34+CD45low和CD34+CD45low细胞中,CD95与Bcl-2表达均呈负相关(r分别为-0.93和-0.81,P<0.05).结论 再生障碍性贫血模型小鼠中,骨髓中造血细胞的比例下降,有核细胞和CD34+细胞的CD95表达比例升高,凋亡明显,针对不同的凋亡指标进行相应调节,可能是有效治疗再生障碍性贫血的途径之一.
目的 探討再生障礙性貧血模型小鼠骨髓造血細胞凋亡過程中CD95和Bcl-2的錶達以及相互之間的關繫.方法 用輻射和免疫介導的方法 製造小鼠的再生障礙性貧血模型.用流式細胞術分析模型組和正常對照組CD34+細胞和CD45+細胞,以及細胞錶麵CD95和胞內Bcl-2的錶達.結果 模型組骨髓細胞中低錶達CD45的CD34+細胞(CD34+CD45low細胞)比例低于對照組[(0.85±0.19)%比(1.21±0.28)%,P<0.05].有覈細胞中,CD95在模型組中的錶達比例明顯高于對照組[(66.30±7.68)%比(42.78±15.12)%],在這2組CD34+CD45+細胞的錶達分彆為(69.21±9.36)%和(22.31±3.33)%,CD34+CD45low細胞的錶達分彆為(53.70±16.46)%和(7.68±4.42)%,均P<0.01.Bcl-2的錶達比例僅在模型組CD34+CD45low細胞中增加,與對照組相比差異有統計學意義[(7.60±2.40)%比(0.70±0.64)%,P<0.05].對照組細胞的CD95與Bcl-2錶達無相關,而模型組CD34+CD45low和CD34+CD45low細胞中,CD95與Bcl-2錶達均呈負相關(r分彆為-0.93和-0.81,P<0.05).結論 再生障礙性貧血模型小鼠中,骨髓中造血細胞的比例下降,有覈細胞和CD34+細胞的CD95錶達比例升高,凋亡明顯,針對不同的凋亡指標進行相應調節,可能是有效治療再生障礙性貧血的途徑之一.
목적 탐토재생장애성빈혈모형소서골수조혈세포조망과정중CD95화Bcl-2적표체이급상호지간적관계.방법 용복사화면역개도적방법 제조소서적재생장애성빈혈모형.용류식세포술분석모형조화정상대조조CD34+세포화CD45+세포,이급세포표면CD95화포내Bcl-2적표체.결과 모형조골수세포중저표체CD45적CD34+세포(CD34+CD45low세포)비례저우대조조[(0.85±0.19)%비(1.21±0.28)%,P<0.05].유핵세포중,CD95재모형조중적표체비례명현고우대조조[(66.30±7.68)%비(42.78±15.12)%],재저2조CD34+CD45+세포적표체분별위(69.21±9.36)%화(22.31±3.33)%,CD34+CD45low세포적표체분별위(53.70±16.46)%화(7.68±4.42)%,균P<0.01.Bcl-2적표체비례부재모형조CD34+CD45low세포중증가,여대조조상비차이유통계학의의[(7.60±2.40)%비(0.70±0.64)%,P<0.05].대조조세포적CD95여Bcl-2표체무상관,이모형조CD34+CD45low화CD34+CD45low세포중,CD95여Bcl-2표체균정부상관(r분별위-0.93화-0.81,P<0.05).결론 재생장애성빈혈모형소서중,골수중조혈세포적비례하강,유핵세포화CD34+세포적CD95표체비례승고,조망명현,침대불동적조망지표진행상응조절,가능시유효치료재생장애성빈혈적도경지일.
Objective To investigate the expressions of CD95 and Bcl- 2 in bone marrow haemopoietic cells from mice with aplastic anemia and their relationship. Methods The mouse model of aplastic anemia was established by irradiation and immuno-mediation. Flow cytometry was employed to analyze the CD34+ cells, CD45+ cells, as well as expressions of surface CD95 and intracellular Bcl-2. Results The ratio of CD34+ cells with low expresson of CD45 (CD34+ CD45low) cells in the bone marrow was significantly lower in model group than that in control group [(0.85 ± 0.19)% vs (1.21±0.28)%, P< 0.05]. The ratio of CD95 expression in model group was increased significantly on nucleated cells compared to control group [(66.30±7.68)% vs (42.78±15.12)%], on CD34+CD45+ cells [(69.21±9.36)% vs (22.31± 3.33)%], and on CD34+CD45low cells [(53.70±16.46)% vs (7.68±4.42)%], respectively, all P<0.01. The ratio of Bcl-2 expression appeared comparable on different cells except on CD34+CD45low cells, which was (7.60±2.40)% in the model group and (0.70±0.64)% in control group with significant difference (P<0.05). The expressions of CD95 was not correlated with Bcl-2 in control group, but CD95 was negatively correlated with Bcl-2 on CD34+CD45+ celIs (r=-0.93, P<0.05)and CD34+CD45low cells (r=-0.81, P<0.05) of model group. Conclusions Mice with aplastic anemia showed lower ratio of haemopoietic cells, higher expression ratio of CD95 on nucleated and CD34+ cells in bone marrow, and obvious apoptosis. Regulation targeting at different markers of apoptosis may be helpful for effective treatment of aplastic anemia.
