CAJ | 학술논문

目的观察细胞因子新的组合(rhG-CSF+rhIL-11+rhIL-2)对骨髓型急性放射病(ARS)比格犬的治疗作用.方法以4.5 Gy60 Coγ射线照射比格犬制备骨髓型ARS动物模型,动物分为照射对照(5只)、综合对症(5只)和综合对症加细胞因子治疗3组(6只),通过观察动物体征、存活时间、存活数以及动物造血及胃肠道等脏器的恢复情况,分析细胞因子联合治疗效果.结果 4.5 Gy60 Coγ射线照射后,比格犬出现食欲下降、发热、精神状态差和柏油便等症状,照射对照组动物于照射后2周内全部死亡,平均存活(12.7±1.4)d;综合对症组呈现明显的急性放射病症状,并于照射后第33天死亡1只;而综合对症加细胞因子组动物在45 d观察期内不仅全部存活,临床症状明显改善,且其造血功能及胃、肠道等受损组织基本恢复.结论在综合对症基础上给予rhG-CSF+rhIL-11+rhIL-2组合治疗可以有效地促进4.5 Gy60 Coγ射线照射比格犬造血系统功能和胃肠道等重要脏器的恢复,提高受照动物存活数,并改善动物生存质量.
목적 관찰세포인자신적조합(rhG-CSF+rhIL-11+rhIL-2)대골수형급성방사병(ARS)비격견적치료작용.방법 이4.5 Gy60 Coγ사선조사비격견제비골수형ARS동물모형,동물분위조사대조(5지)、종합대증(5지)화종합대증가세포인자치료3조(6지),통과관찰동물체정、존활시간、존활수이급동물조혈급위장도등장기적회복정황,분석세포인자연합치료효과.결과 4.5 Gy60 Coγ사선조사후,비격견출현식욕하강、발열、정신상태차화백유편등증상,조사대조조동물우조사후2주내전부사망,평균존활(12.7±1.4)d;종합대증조정현명현적급성방사병증상,병우조사후제33천사망1지;이종합대증가세포인자조동물재45 d관찰기내불부전부존활,림상증상명현개선,차기조혈공능급위、장도등수손조직기본회복.결론 재종합대증기출상급여rhG-CSF+rhIL-11+rhIL-2조합치료가이유효지촉진4.5 Gy60 Coγ사선조사비격견조혈계통공능화위장도등중요장기적회복,제고수조동물존활수,병개선동물생존질량.
Objective To evaluate the effects of combined administration of recombinant human interleukin-11(rhIL-11),recombinant human G-CSF(rhG-CSF)and recombinant human interleukin-2 (rhIL-2)on acute radiation sickness(ARS)beagles.Methods Sixteen beagle were irradiated with 4.5 Gy60 Co γ-rays to establish ARS models,and were divided into irradiation control group,supportive care group and combined cytokines treatment group.After irradiation irradiation control group was given no treatment,the dogs in supportive care group received purely symptomatic treatment,while combined cytokines treatment group received rhIL-11 50μg/(kg·d)and rbG-CSF 10μg/(kg·d)subcutaneously(0-14 d)and rhIL-2 1×1 06 U/d(29-43 d)besides symptomatic treatment.Manifestation and characteristics of ARS beagles were observed,and the survival time were recorded.At last,post-mortem examination and histological examination were performed.Results All animals underwent nausea,diarrhea and fever.After irradiation,all animals in irradiation control group died in two weeks,and the mean survival time was 12.7 d,while only one died at 33 d in supportive care group.All dogs in combined cytokine group survived at 45 day after exposure,and their haematopoiesis and gastrointestinal tract were recovered.Conclusions Combination of rhIL-11 + rhG-CSF + rhIL-2 treatment could be significantly effective on ARS beagles irradiated by 4.5 Gy60 Co γ-rays,which could accelerate injured haemotopoiesis and intestinal tract recovery,increase the survival rate and improve the life quality of animals.