CAJ | 학술논문

目的探索IL-6在实验性自身免疫性脑脊髓炎(EAE)中的作用机制.方法将25只雌性Wistar大鼠随机分为EAE组(n=15)、对照组(n=10).利用免疫组化染色技术观察IL-6、TNF-α、TGF-β的表达,进行相关性分析.结果 IL-6灰度值与症状评分呈负相关(r=-0.953,P<0.05)、与TNF-α呈正相关(r=0.967,P<0.05),IL-6与TGF·B呈无相关(r=-0.492,P>0.05).TNF-α灰度值与症状评分呈负相关(r=-0.978,P<0.05),TNF-α与TGF-β无相关(r=-0.502,P>0.05).TGF-β灰度值与症状评分无相关(r=0.470,P>0.05).结论 IL-6参与EAE的过程,可能是一种致炎因子.
목적 탐색IL-6재실험성자신면역성뇌척수염(EAE)중적작용궤제.방법 장25지자성Wistar대서수궤분위EAE조(n=15)、대조조(n=10).이용면역조화염색기술관찰IL-6、TNF-α、TGF-β적표체,진행상관성분석.결과 IL-6회도치여증상평분정부상관(r=-0.953,P<0.05)、여TNF-α정정상관(r=0.967,P<0.05),IL-6여TGF·B정무상관(r=-0.492,P>0.05).TNF-α회도치여증상평분정부상관(r=-0.978,P<0.05),TNF-α여TGF-β무상관(r=-0.502,P>0.05).TGF-β회도치여증상평분무상관(r=0.470,P>0.05).결론 IL-6삼여EAE적과정,가능시일충치염인자.
Objective To explore the mechanism of IL-6 in EAE. Methods 25 female Wistar rats were randomly divided into 2 groups, EAE group( n=15 ) and normal control group( n=10). The expression of IL-6, TNF-αtand TGF-β of the two groups rots were ob-served by immunohistochemistry staining, and then correlation analysis of the expression of IL-6, TNF-αtandTGF-β was made. Results There was negative correlation between gray scale of IL-6 and symptom scores( r=-0.953, P＞0.05). IL-6 and TGF-β had no statistical-ly significant correlation ( r=-0.492, P＞0.05). There was negative correlation between gray scale of TNF-α and symptom scores( r=-0.978, P＜0.05), and both of the correlation between TNF-α and TGF-β ( r=-0.502, P＞0.05), TGF-β and scores( r=0.470,P＞0.05 ) were not statistically significant. Conclusions IL-6 may participate in EAE as a inflammatory factor.