中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2010年
5期
326-328
,共3页
倪晓光%白晓枫%王贵齐%刘尚梅%郭冰%赵平
倪曉光%白曉楓%王貴齊%劉尚梅%郭冰%趙平
예효광%백효풍%왕귀제%류상매%곽빙%조평
胰腺肿瘤%膜联蛋白A1%增殖细胞核抗原%组织芯片
胰腺腫瘤%膜聯蛋白A1%增殖細胞覈抗原%組織芯片
이선종류%막련단백A1%증식세포핵항원%조직심편
Pancreatic neoplasms%Annexin A1%Proliferating cell nuclear antigen%Tissue microarray
目的 研究膜联蛋白A1(annexin A1,ANXA1)在胰腺癌中的表达及其与增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的相关性.方法 应用组织芯片制作仪构建芯片,芯片上有39例正常胰腺组织,42例胰腺癌组织,7例胰岛细胞瘤组织,8例壶腹腺癌组织.在连续的芯片切片上使用免疫组化法检测ANXA1和PCNA蛋白的表达,分析两者的相关性.结果 构建的组织芯片在免疫组化染色后的掉片率为11.7%～13.3%,满足实验要求.ANXA1在胰腺癌中的阳性表达率为71.4%(30/42),明显高于正常胰腺组织18.4%(7/38)的阳性率(P<0.01).PCNA在胰腺癌中的阳性表达率为73.8%(31/42),也明显高于正常胰腺组织22.2%(8/36)的阳性率(P<0.01).胰腺癌组织ANXA1表达与PCNA表达显著相关(P<0.01).结论 胰腺癌ANXA1蛋白的表达与PCNA表达同步增加.
目的 研究膜聯蛋白A1(annexin A1,ANXA1)在胰腺癌中的錶達及其與增殖細胞覈抗原(proliferating cell nuclear antigen,PCNA)的相關性.方法 應用組織芯片製作儀構建芯片,芯片上有39例正常胰腺組織,42例胰腺癌組織,7例胰島細胞瘤組織,8例壺腹腺癌組織.在連續的芯片切片上使用免疫組化法檢測ANXA1和PCNA蛋白的錶達,分析兩者的相關性.結果 構建的組織芯片在免疫組化染色後的掉片率為11.7%～13.3%,滿足實驗要求.ANXA1在胰腺癌中的暘性錶達率為71.4%(30/42),明顯高于正常胰腺組織18.4%(7/38)的暘性率(P<0.01).PCNA在胰腺癌中的暘性錶達率為73.8%(31/42),也明顯高于正常胰腺組織22.2%(8/36)的暘性率(P<0.01).胰腺癌組織ANXA1錶達與PCNA錶達顯著相關(P<0.01).結論 胰腺癌ANXA1蛋白的錶達與PCNA錶達同步增加.
목적 연구막련단백A1(annexin A1,ANXA1)재이선암중적표체급기여증식세포핵항원(proliferating cell nuclear antigen,PCNA)적상관성.방법 응용조직심편제작의구건심편,심편상유39례정상이선조직,42례이선암조직,7례이도세포류조직,8례호복선암조직.재련속적심편절편상사용면역조화법검측ANXA1화PCNA단백적표체,분석량자적상관성.결과 구건적조직심편재면역조화염색후적도편솔위11.7%～13.3%,만족실험요구.ANXA1재이선암중적양성표체솔위71.4%(30/42),명현고우정상이선조직18.4%(7/38)적양성솔(P<0.01).PCNA재이선암중적양성표체솔위73.8%(31/42),야명현고우정상이선조직22.2%(8/36)적양성솔(P<0.01).이선암조직ANXA1표체여PCNA표체현저상관(P<0.01).결론 이선암ANXA1단백적표체여PCNA표체동보증가.
Objective To investigate the expressions of the annexin A1 ( ANXA1 ) in pancreatic cancer and the correlations with proliferating cell nuclear antigen (PCNA). Methods Tissue microarray instrument was used to construct the chip. There were 39 samples of normal pancreas tissue, 42 samples of pancreatic cancer, 7 samples of islet cell tumor and 8 samples of ampullary carcinoma. The expressions of ANXA1 and PCNA protein were examined by immunohistochemistry and their correlation was analyzed. Results The off-chip rate of tissue chips after immunohistochemistry was 11.7% ～ 13.3%, which could satisfy the needs for experiment. Immunohistochemical results of pancreatic cancer tissue microarray showed that the positive rate of ANXA1 expression in pancreatic cancer was 71.4% (30/42), which was significantly higher when compared with that of normal pancreas tissue ( 18.4%, 7/38; P < 0. 01 ). The positive rate of PCNA expression in pancreatic cancer was 73.8% (31/42), which was significantly higher than that of normal pancreas tissue (22.2%, 8/36; P < 0.01 ). The expression of ANXA1 had a significant correlation with PCNA in pancreatic cancer ( P < 0.01 ). Conclusions The expression of ANXA1 protein was significantly increased in pancreatic cancer. The expression of ANXA1 was significantly correlated with the expression of PCNA.
