中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2011年
5期
286-291
,共6页
唐锋%何星星%常莹%张家云%陈智%汪静%王俊帅%刘翩%唐学军%林菊生
唐鋒%何星星%常瑩%張傢雲%陳智%汪靜%王俊帥%劉翩%唐學軍%林菊生
당봉%하성성%상형%장가운%진지%왕정%왕준수%류편%당학군%림국생
肝炎,乙型,慢性%多态性,单核苷酸%配体%基因型%启动区(遗传学)%肝炎表面抗原,乙型
肝炎,乙型,慢性%多態性,單覈苷痠%配體%基因型%啟動區(遺傳學)%肝炎錶麵抗原,乙型
간염,을형,만성%다태성,단핵감산%배체%기인형%계동구(유전학)%간염표면항원,을형
Hepatitis B,chronic%Polymorphisms,single nucleotide%Ligands%Gene type%Promoter regions (genetics)%Hepatitis B surface antigens
目的 探讨中国汉族人Fas配体(FasL)基因单核苷酸多态性与重型乙型肝炎之间的关系.方法 采用病例一对照研究方法,将HBV感染者作为研究对象.收集相关的非活动性HBsAg携带者233例,重型肝炎患者68例,HBV自发清除者100例,肝硬化患者102例,肝细胞癌患者112例的全血标本及临床相关资料.应用TaqMan探针实时荧光分型PCR方法检测患者FasL基因-844T/C位点的基因多态性,计算比值比(OR)值及95%可信区间(CI).结果 应用二元Logistic回归分析,控制年龄、性别等因素后,非活动性HBsAg携带者的FasL-844 CC、CT、TT基因型频率分别为50.64%、39.91%及9.44%;重型肝炎组中FasL-844 CC、CT、TT基因型频率分别为79.41%、17.65%及2.94%.携带FasL-844CC基因型的非活动性HBsAg携带者与重型肝炎的发生呈显著关联(OR=4.729,95%CI:0.510～21.282,P=0.043),而其他病例对照组中存在的关联差异均无统计学意义(P>0.05).结论 携带FasL-844CC基因型的非活动性HBsAg携带者易发生乙型肝炎重症化,应对这部分人群给予更多的关注.
目的 探討中國漢族人Fas配體(FasL)基因單覈苷痠多態性與重型乙型肝炎之間的關繫.方法 採用病例一對照研究方法,將HBV感染者作為研究對象.收集相關的非活動性HBsAg攜帶者233例,重型肝炎患者68例,HBV自髮清除者100例,肝硬化患者102例,肝細胞癌患者112例的全血標本及臨床相關資料.應用TaqMan探針實時熒光分型PCR方法檢測患者FasL基因-844T/C位點的基因多態性,計算比值比(OR)值及95%可信區間(CI).結果 應用二元Logistic迴歸分析,控製年齡、性彆等因素後,非活動性HBsAg攜帶者的FasL-844 CC、CT、TT基因型頻率分彆為50.64%、39.91%及9.44%;重型肝炎組中FasL-844 CC、CT、TT基因型頻率分彆為79.41%、17.65%及2.94%.攜帶FasL-844CC基因型的非活動性HBsAg攜帶者與重型肝炎的髮生呈顯著關聯(OR=4.729,95%CI:0.510～21.282,P=0.043),而其他病例對照組中存在的關聯差異均無統計學意義(P>0.05).結論 攜帶FasL-844CC基因型的非活動性HBsAg攜帶者易髮生乙型肝炎重癥化,應對這部分人群給予更多的關註.
목적 탐토중국한족인Fas배체(FasL)기인단핵감산다태성여중형을형간염지간적관계.방법 채용병례일대조연구방법,장HBV감염자작위연구대상.수집상관적비활동성HBsAg휴대자233례,중형간염환자68례,HBV자발청제자100례,간경화환자102례,간세포암환자112례적전혈표본급림상상관자료.응용TaqMan탐침실시형광분형PCR방법검측환자FasL기인-844T/C위점적기인다태성,계산비치비(OR)치급95%가신구간(CI).결과 응용이원Logistic회귀분석,공제년령、성별등인소후,비활동성HBsAg휴대자적FasL-844 CC、CT、TT기인형빈솔분별위50.64%、39.91%급9.44%;중형간염조중FasL-844 CC、CT、TT기인형빈솔분별위79.41%、17.65%급2.94%.휴대FasL-844CC기인형적비활동성HBsAg휴대자여중형간염적발생정현저관련(OR=4.729,95%CI:0.510～21.282,P=0.043),이기타병례대조조중존재적관련차이균무통계학의의(P>0.05).결론 휴대FasL-844CC기인형적비활동성HBsAg휴대자역발생을형간염중증화,응대저부분인군급여경다적관주.
Objective To investigate the relationship between single nucleotide polymorphism (SNP) of Fas ligand (FasL) and fulminant hepatitis B in Han Chinese. Methods HBV infected subjects were enrolled in this case-control study, including 233 cases of inactive HBsAg carrier, 68 patients with fulminant hepatitis B,100 cases of spontaneous hepatitis B clearance, 102 patients with hepatitis B virus (HBV) related cirrhosis and 112 patients with HBV related primary hepatocellular carcinoma. The blood samples and clinical data were collected. FasL-844T/C polymorphisms of enrolled subjects were examined by TaqMan real time fluorescent genotyping polymerase chain reaction (RT-PCR). A adjusted odds ratios (OR)and 95% confidence intervals (CI)were calculated using the Logistic regression model. Results After adjusting the factors of gender and age, binary Logistic regression analyses indicated that the genotype frequencies of FasL-844 CC,CT,TT in inactive HBsAg carriers were 50. 64% ,39. 91% and 9. 44% respectively, and those in cases of fulminant hepatitis B were 79. 41%, 17. 65% and 2. 94%, respectively. The analysis also revealed that FasL-844CC genotype in inactive HBsAg carriers was high risk factor of developing fulminant hepatitis B (OR =4. 729,95%CI:0. 510 - 21. 282,P = 0. 043), while there were no statistic significances in other cases (P>0. 05). Conclusion The inactive HBsAg carriers harboring FasL-844CC may have greater susceptibility to fulminant hepatitis B, which need arouse high attention.
