CAJ | 학술논문

目的探讨人乳头瘤病毒(human papillomavirus,HPV)与我国河北省保定地区食管癌发生的相关性.方法应用HPV LI通用引物GP5+,6+、HPV16 E6和HPV18 E6型特异性引物多聚酶链反应(Polymerase chain reaction,PCR),检测保定地区食管癌组织中HPV存在状况.结果 42例食管癌组织中,37例检测到HPV阳性,阳性率为88.1%;其中19例检测到HPV16 E6基因,阳性率为45.2%,8例为HPV18 E6基因阳性,阳性率为19.0%,5例HPV16 E6和18 E6基因均阳性,为混合感染.结论我国河北省保定地区食管癌组织中有HPV存在,并且HPV感染可能是食管癌发生的重要影响因子.
목적 탐토인유두류병독(human papillomavirus,HPV)여아국하북성보정지구식관암발생적상관성.방법 응용HPV LI통용인물GP5+,6+、HPV16 E6화HPV18 E6형특이성인물다취매련반응(Polymerase chain reaction,PCR),검측보정지구식관암조직중HPV존재상황.결과 42례식관암조직중,37례검측도HPV양성,양성솔위88.1%;기중19례검측도HPV16 E6기인,양성솔위45.2%,8례위HPV18 E6기인양성,양성솔위19.0%,5례HPV16 E6화18 E6기인균양성,위혼합감염.결론 아국하북성보정지구식관암조직중유HPV존재,병차HPV감염가능시식관암발생적중요영향인자.
Objective To investigate the relationship between the human papillomavirus (HPV) and esophageal carcinoma in Baoding City of Hebei Province. Methods We detected HPV DNA in 42 formalin-fixed and paraffin-embedded tissues from surgically resected esophageal carcinomas from Baoding City of Hebei Province, by PCR with the general primer set of GP5 +/6 + for HPV L1 gene and type-specific primer sets for HPV16 and 18 as well. Results 37 from 42 esophageal carcinoma samples were HPV positive and the rate was 88.1% . Among the samples detected, 19 were HPV16 E6 positive and rate was 45.2%, eight were HPV18 E6 positive and rate was 19.0%. Conclusion The high rate of HPV in the esophageal carcinoma samples suggested that HPV plays an etiologic role in the development of esophageal cancer in Baoding City of Hebei Province.