CAJ | 학술논문

目的用肿瘤组织块直接接种法建立人脑肿瘤裸小鼠原位移植模型,通过分析移植瘤的生物学特征来证明本操作方法的可行性. 方法取人肺腺癌脑转移瘤的新鲜组织或人脑多形性胶质母细胞瘤(GBM)裸小鼠皮下移植瘤组织,剪成小块,置入专用套管针,通过事先钻好的颅骨孑孔,徒手推入裸小鼠右尾状核.观察致瘤率、移植瘤的组织细胞形态、相关标志物、MRI图像特征和荷瘤鼠生存期. 结果肺腺癌脑转移瘤和GBM组织在鼠与鼠之间分别传了6代和13代.荷瘤鼠生存期分别为(38.0±0.9)d和(19.0±1.3)d.肺腺癌脑转移瘤移植瘤不向周围正常鼠脑组织侵袭,分泌酸性粘液及表达癌胚抗原等生物学特征与其亲本肿瘤一致:而GBM移植瘤具有向周围正常鼠脑组织高度侵袭及高表达表皮生长因子受体的生物学特征也与其亲本肿瘤一致. 结论肿瘤组织块直接注入裸小鼠脑内建立的实验动物模型比肿瘤细胞悬液接种方法相对简易.且移植瘤能更好地保持亲本肿瘤的特征.此法可进一步在各种腩肿瘤动物原位移植实验中推广应用.
목적 용종류조직괴직접접충법건립인뇌종류라소서원위이식모형,통과분석이식류적생물학특정래증명본조작방법 적가행성. 방법 취인폐선암뇌전이류적신선조직혹인뇌다형성효질모세포류(GBM)라소서피하이식류조직,전성소괴,치입전용투관침,통과사선찬호적로골혈공,도수추입라소서우미상핵.관찰치류솔、이식류적조직세포형태、상관표지물、MRI도상특정화하류서생존기. 결과폐선암뇌전이류화GBM조직재서여서지간분별전료6대화13대.하류서생존기분별위(38.0±0.9)d화(19.0±1.3)d.폐선암뇌전이류이식류불향주위정상서뇌조직침습,분비산성점액급표체암배항원등생물학특정여기친본종류일치:이GBM이식류구유향주위정상서뇌조직고도침습급고표체표피생장인자수체적생물학특정야여기친본종류일치. 결론 종류조직괴직접주입라소서뇌내건립적실험동물모형비종류세포현액접충방법 상대간역.차이식류능경호지보지친본종류적특정.차법가진일보재각충남종류동물원위이식실험중추엄응용.
Objective To establish a method for direct orthotopic transplantation of human brain tumors into mouse brain and assess the parental characteristics of the xenografts. Methods Fresh tissues of human brain metastasis of lung adenocarcinoma and glioblastoma tissues subcutaneously implanted in nude mice were harvested and minced into pieces. A special trochar was used to inject the tissue pieces into the right caudate nuclei of nude mice through the burr hole in the mouse skull. The efficiency of tumorigenicity, survival time of tumor-bearing mice, histopathological features, expressions of tumor markers in the xenografts and MRI findings were observed. Results The metastatic lung adenocarcinoma and glioma xenografts were passaged for 6 and 13 generations in nude mice, and the average survival time of the tumor-bearing mice was 38.0±0.9 days and 19.0±1.3 days, respectively. The metastatic xenogratts were characterized by expressions of CEA and acid mucin without invasiveness to the normal recipient brain tissue, which were consistent with the features of the parental metastatic tumor. The glioma xenogratts exhibited deep invasiveness to the normal recipient brain tissue and high expression of EGFR, which were identical to the characteristics of the parental tumor. Conclusion Compared with stereotactic cell suspension injection, direct tumor tissue graft injection requires simple procedures and well maintains the characteristics of the parental tumor tissue in the xenograft in nude mice. This method well suits the purposes of establishing orthotopic xenotransplantation models of brain tumors in nude mice.