CAJ | 학술논문

目的探讨核仁组成区嗜银蛋白(AgNOR)及细胞增殖指数(PIKi-67)在乳腺癌前驱病变中的意义。方法将76例乳腺癌前驱病变,包括导管上皮不典型增生( ADH) 25例、低度导管原位癌(DCIS) 17例、中度导管原位癌14例及高度导管原位癌20例,制成组织芯片,进行AgNOR嗜银染色及Ki-67免疫组织化学染色,图像定量分析AgNOR染色结果并计算PIKi-67。结果 AgNOR颗粒形态、大小在各病变中存在差异;每个细胞AgNOR颗粒平均个数、AgNOR颗粒总面积、单个Ag-NOR颗粒面积、PI Ki-67从ADH到高度DCIS逐渐增高,ADH、低度DCIS与高度DCIS的差异均有统计学意义(P＜0.01),ADH与低度DCIS在每个细胞AgNOR颗粒平均个数上的差异有统计学意义(P＜0.05)。结论 AgNOR与Ki-67是乳腺癌前驱病变诊断及鉴别诊断的指标之一。
목적 탐토핵인조성구기은단백(AgNOR)급세포증식지수(PIKi-67)재유선암전구병변중적의의。방법 장76례유선암전구병변,포괄도관상피불전형증생( ADH) 25례、저도도관원위암(DCIS) 17례、중도도관원위암14례급고도도관원위암20례,제성조직심편,진행AgNOR기은염색급Ki-67면역조직화학염색,도상정량분석AgNOR염색결과병계산PIKi-67。결과 AgNOR과립형태、대소재각병변중존재차이;매개세포AgNOR과립평균개수、AgNOR과립총면적、단개Ag-NOR과립면적、PI Ki-67종ADH도고도DCIS축점증고,ADH、저도DCIS여고도DCIS적차이균유통계학의의(P＜0.01),ADH여저도DCIS재매개세포AgNOR과립평균개수상적차이유통계학의의(P＜0.05)。결론 AgNOR여Ki-67시유선암전구병변진단급감별진단적지표지일。
Objective To study the diagnostic and differential diagnostic significance of argyrophilic nucleolar organizer region (AgNOR) and cellular proliferation index ( PlKi-67 ) in the precursor lesions of the breast carcinoma. Methods Image quantitative analysis of AgNOR and immunostains of Ki-67 in tissue microarray sections from 76 biopsies of the precursor lesions of the breast carcinoma. Results There were some differences in granulae sizes and shapes of AgNOR among the precursor lesions of the breast carcinoma. The number of AgNORs per nucleu, total area of AgNORs, mean area of a single AgNOR, PIKi-67 were increasing from ADH to high-grade DCIS, and there was statistical difference between ADH, low-grade DCIS,respectively and high-grade DCIS. The number of AgNORs per nucleu was statistically different between ADH and low-grade DCIS. Conclusion AgNOR and Ki-67 could be regarded as assistant indicators in the diagnosis and differential diagnosis of the precursor lesions of the breast carcinoma.