CAJ | 학술논문

目的探讨骨架相关蛋白Transgelin在伴或不伴糖尿病的胰腺癌组织中的表达，及其对胰腺癌SW1990细胞运动侵袭的影响。方法采用免疫组织化学法检测Transgelin在92例胰腺癌患者（其中伴糖尿病者45例）的癌组织、癌旁组织（距癌边缘＞5 cm）中的表达水平，分析其与临床病理特征的关系；设计并体外合成靶向Transgelin的小干扰RNA(siRNA)，将其转染到SW1990细胞中，采用Transwell小室检测转染前后细胞运动侵袭能力的改变。结果 Transgelin在胰腺癌组织中的表达阳性率为68.5％(63/92),显著高于癌旁组织[33.7％(31/92)，P＜o.05]。伴随糖尿病的胰腺癌组织中Transgelin表达阳性率为84.4％(38/45)，显著高于无糖尿病的胰腺癌组[53.2％(25/47)，P＜0.05]。胰腺癌组织中Transgelin表达与胰腺癌的淋巴结转移及TNM分期密切相关(P＜0.05)，与性别、年龄、发生部位、分化程度、门静脉或神经受侵犯无关(P＞0.05)。Transgelin siRNA干扰后48 h，SW1990细胞迁移能力[穿膜细胞数为(49.2±9.5)个]明显低于阴性对照组和空白组[(61.9±7.5)和(65.3±10.6)个，P值均＜0.05];SW1990细胞的体外侵袭能力[穿膜细胞数为(48.0±8.6)个]也明显低于阴性对照组和空白组[（63.5±11.4）个和(67.5±9.6)个,P值均＜0.05]。结论 Transgelin可能通过促进胰腺癌细胞的运动侵袭能力，参与伴随糖尿病的胰腺癌的转移发生。
목적 탐토골가상관단백Transgelin재반혹불반당뇨병적이선암조직중적표체，급기대이선암SW1990세포운동침습적영향。방법 채용면역조직화학법검측Transgelin재92례이선암환자（기중반당뇨병자45례）적암조직、암방조직（거암변연＞5 cm）중적표체수평，분석기여림상병리특정적관계；설계병체외합성파향Transgelin적소간우RNA(siRNA)，장기전염도SW1990세포중，채용Transwell소실검측전염전후세포운동침습능력적개변。결과 Transgelin재이선암 조직중적표체양성솔위68.5％(63/92),현저고우암방조직[33.7％(31/92)，P＜o.05]。반수당뇨병적이선암조직중Transgelin표체양성솔위84.4％(38/45)，현저고우무당뇨병적이선암조[53.2％(25/47)，P＜0.05]。이선암조직중Transgelin표체여이선암적림파결전이급TNM분기밀절상관(P＜0.05)，여성별、년령、발생부위、분화정도、문정맥혹신경수침범무관(P＞0.05)。Transgelin siRNA간우후48 h，SW1990세포천이능력[천막세포수위(49.2±9.5)개]명현저우음성대조조화공백조[(61.9±7.5)화(65.3±10.6)개，P치균＜0.05];SW1990세포적체외침습능력[천막세포수위(48.0±8.6)개]야명현저우음성대조조화공백조[（63.5±11.4）개화(67.5±9.6)개,P치균＜0.05]。결론 Transgelin가능통과촉진이선암세포적운동침습능력，삼여반수당뇨병적이선암적전이발생。
Objective To investigate the expression of actin-associated protein Transgelin in pancreatic cancer with or without diabetes and its effects on migration and invasion in SW1990 cell line. Methods The expression of Transgelin in 92 pancreatic cancer tissue specimens (45 cases accompanied with diabetes) and adjacent tumor-free tissue specimens (over 5cm from the edge of the tumor) was detected by immunohistochemistry, and their association with clinical pathological characteristics were also analyzed. Transgelin siRNA was designed and transfected into pancreatic cancer cell line SW1990. The changes of migration and invasion before and after transfection were observed through Transwell test.Results The positive percentage of Transgelin expression in pancreatic cancer was 68.5 ％ (63/92), which was significantly higher than that of adjacent tumor-free tissues[33.7％ ( 31/92), P＜ 0.05]. The positive percentage of Transgelin expression in pancreatic cancer accompanied with diabetes was 84.4％ (38/45), which was significantly higher than that without diabetes[53.2％ (25/47), P＜0.05]. The expression of Transgelin in pancreatic cancer tissues was associated with lymph nodes metastasis and TNM staging (both P＜0.05), but not related with gender, age, site, differentiation and portal vein or nerve invasion (P＞0.05). After Transgelin was interfered for 48 hours, the migration ability was significantly lower (migration cell number 49.2 ±9.5 cells) than negative control group (61.9±7.5 cells) and blank group (65.3±10.6 cells) (both P＜0.05), and the invasion of SW 1990 cells (48.0 ± 8.6 cells) also significantly lower than negative control group (63.5±11.4 cells) and blank group (67.5±9.6 cells) (both P＜0. 05). Conclusion Transgelin may involve in the metastasis of pancreatic cancer accompanied with diabetes through promoting pancreatic cancer cell migration and invasion.